Stefan Bohn

PA200 modulates immunoproteasome structure and activity

Dušan Živković, Amélie Bosc-Rosati, Angélique Sanchez Dafun, Fatme Mourtada et autres

The proteasome activator PA200 binds to the catalytic core of both standard proteasome (s20S) and the immunoproteasome (i20S); however, whether PA200 uses the same mechanisms to activate i20S remains unknown. In this work, the cryo-EM structures of singly- and doubly-capped i20S-PA200 complexes, …

fr, gb, de, pl (code pays fourni par la source)

Architecture of the 8 MDa Hdr–Vhu–Fwd super-assembly in class I methanogens

Sophia Paul, Tomas C. Pascoa, Max A. Klamke, Stefan Bohn et autres

Abstract Methanogens are central to global carbon cycling and among the largest biological sources of methane, a potent greenhouse gas 1 . At the heart of their energy metabolism lies the Hdr–Vhu–Fwd super-assembly, which couples H 2 oxidation with CO 2 reduction …

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Structural Basis of Membrane Potential Coupled Vectorial CO 2 Hydration by the DAB2 Complex in Chemolithoautotrophs

Yat Kei Lo, Michael Seletskiy, Stefan Bohn, Darja Deobald et autres

Abstract The fixation of dissolved inorganic carbon (DIC) such as CO 2 and bicarbonate by autotrophic microorganisms is fundamental to the global primary production. Many autotrophs depend on a diversity of CO 2 -concentrating mechanisms (CCMs) to overcome the inefficiency of ribulose-1,5-bisphosphate …

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Cryo-EM structure of locked spike glycoprotein from bat SARS-like coronavirus WIV1, molecular dynamics and biophysics across host range

Chuan Liu, Jingjing Zheng, Yuhan Wang, F. Beck et autres

As made evident by severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1) and SARS-CoV-2 pandemics, the possibility of a SARS-like coronavirus (SL-CoV) emerging again in humankind after zoonotic spillover represents a significant global health threat. Given the role of spike (S) glycoprotein in …

cn, de, hu (code pays fourni par la source)

Structure of the Methanosarcina mazei Mtr complex bound to the oxygen-stress responsive small protein MtrI

Tristan Reif-Trauttmansdorff, Eva Herdering, Stefan Bohn, Tomas C. Pascoa et autres

Methanogenic archaea emit ~1 Gt of methane annually, impacting global carbon cycling and climate. Central to their energy metabolism is a membrane-bound, sodium-translocating methyltransferase complex: the N⁵-tetrahydromethanopterin:CoM-S-methyltransferase (Mtr). It couples methyl transfer between two methanogen-specific cofactors with sodium ion transport across the …

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A histidine switch regulates pH-dependent filament formation by the caspase-9 CARD

Đesika Kolarić, Stefan Bohn, Dagmar Kolb, Tea Pavkov‐Keller et autres

Abstract The caspase activation and recruitment domain (CARD) mediates protein-protein interactions in apoptotic and inflammatory signaling pathways. In humans, more than 30 proteins contain a CARD, several of which have been reported to polymerize into helical filaments. Here we found that the …

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Structure of the energy converting methyltransferase (Mtr) of Methanosarcina maze i in complex with an oxygen-stress responsive small protein

Tristan Reif-Trauttmansdorff, Eva Herdering, Stefan Bohn, Tomas C. Pascoa et autres

Abstract Methanogenic archaea contribute 1–2 Gt of methane annually, impacting both global carbon cycling and climate. Central to their energy metabolism is a membrane-bound, sodium-translocating methyltransferase complex: the N⁵-tetrahydromethanopterin:CoM-S-methyltransferase (Mtr complex), which catalyzes the methyl transfer between two methanogen specific cofactors. This …

de (code pays fourni par la source)

Uncovering the Unexpected Role of Proteasome Activator PA200 in Regulating Immunoproteasome Expression and Activity

Marie‐Pierre Bousquet, Dušan Živković, Angélique Sanchez Dafun, Ayse Seda Yazgili et autres

fr, gb, de, pl (code pays fourni par la source)

Structural insights into late-stage photosystem II assembly by Psb32

Stefan Bohn, Yat Kei Lo, Jan Lambertz, Jakob Meier‐Credo et autres

Abstract Photosystem II (PSII) assembly is a stepwise process in which intermediate complexes with auxiliary proteins are transiently formed to allow efficient de novo biogenesis or repair of damaged PSII. In particular, the role of extrinsic PSII subunits (PsbO, PsbU, PsbV) and …

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Structure of the ATP-driven methyl-coenzyme M reductase activation complex

Fidel Ramírez-Amador, Anuj Kumar, Christian Lorent, Sébastian Keller et autres

Abstract Methyl-coenzyme M reductase (MCR) is the enzyme responsible for nearly all biologically generated methane1. Its active site comprises coenzyme F430, a porphyrin-based cofactor with a central nickel ion that is active exclusively in the Ni(I) state2,3. How methanogenic archaea perform the …

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2-oxoglutarate triggers assembly of active dodecameric Methanosarcina mazei glutamine synthetase

Eva Herdering, Tristan Reif-Trauttmansdorff, Anuj Kumar, Tim Habenicht et autres

Glutamine synthetases (GS) are central enzymes essential for the nitrogen metabolism across all domains of life. Consequently, they have been extensively studied for more than half a century. Based on the ATP-dependent ammonium assimilation generating glutamine, GS expression and activity are strictly …

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Author response: 2-oxoglutarate triggers assembly of active dodecameric Methanosarcina mazei glutamine synthetase

Eva Herdering, Tristan Reif-Trauttmansdorff, Anuj Kumar, Tim Habenicht et autres

The central nitrogen-metabolite 2-oxoglutarate directly promotes glutamine synthetase activity by inducing its dodecameric assembly and priming the active sites, representing a novel mechanism of regulation.

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