Andréanne Lupien

Characterization of Antibiotic Combinations Against Mycobacterium abscessus Using Growth and Viability Assays

Zeineb Haouari, Andréanne Lupien

The Mycobacterium abscessus complex is a rapidly growing group of non-tuberculous mycobacteria associated with severe pulmonary infections and intrinsic antibiotic resistance, limiting treatment options for affected patients. Thus, to maximize efficacy,combination therapy is commonly used in the clinical management of M. abscessus …

Human prostaglandin reductases dearomatize and inactivate benzothiazinone antitubercular drugs

Myriam Briki, Caroline Wagner, Vincent Desfontaine, Anthony Vocat et autres

Abstract Macozinone (MCZ, PBTZ169) is a potent clinical stage benzothiazinone antitubercular agent that covalently inhibits the essential mycobacterial flavoenzyme DprE1. In some mammals, MCZ undergoes reductive dearomatization to H 2 MCZ, a Hydride Meisenheimer Complex, identified as the major circulating metabolite in …

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Large-scale testing of antimicrobial lethality at single-cell resolution predicts mycobacterial infection outcomes

Alexander Jovanovic, Frederick K. Bright, Ahmad Sadeghi, Basil Wicki et autres

In vitro antibiotic testing is important for guiding therapy and drug development. Current methods are focused on growth inhibition in bulk bacterial populations but often fail to accurately predict treatment responses. Here we introduce Antimicrobial Single-Cell Testing (ASCT), a large-scale live-cell imaging …

Large-scale testing of antimicrobial lethality at single-cell resolution predicts mycobacterial infection outcomes

Alexander Jovanovic, Frederick K. Bright, Ahmad Sadeghi, Basil Wicki et autres

In vitro antibiotic testing is important for guiding therapy and drug development. Current methods are focused on growth inhibition in bulk bacterial populations but often fail to accurately predict treatment responses. Here we introduce Antimicrobial Single-Cell Testing (ASCT), a large-scale live-cell imaging …

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Chlorotonils exhibit potent activity against Mycobacterium tuberculosis , while resistance is mediated by MmpR5-MmpL5

Felix Deschner, Melissa Dalcina Chengalroyen, Lauren Ames, Diana Quach et autres

Abstract Treatment of Mycobacterium tuberculosis (Mtb) is challenging and requires administration of at least four different antibiotics. Unfortunately, multi-drug resistant Mtb strains continue to emerge, undermining the effectiveness of current treatment regimens and highlighting the urgent need for new therapeutics. In this …

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Pantetheinamides that inhibit the growth of intracellular Salmonella Typhimurium

Jacob Pierscianowski, Dustin Duncan, Chunling Blue Lan, Rohit Kholiya et autres

Abstract The metabolic adaptability of intracellular pathogens, such as the Gram-negative Salmonella enterica serovar Typhimurium (STm), enables their survival in nutrient-restricted host environments, while also presenting an opportunity for selective antimicrobial targeting. Herein, we report the synthesis and screening of a small …

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Virulence hierarchies within the Mycobacterium tuberculosis complex

Sarah N Danchuk, Shannon C. Duffy, Jaryd Sullivan, Syed Beenish Rufai et autres

The Mycobacterium tuberculosis complex (MTBC) includes M. tuberculosis ( M. tb ), the primary cause of human tuberculosis, M. bovis, the classical zoonotic pathogen and cause of bovine tuberculosis, and M. orygis, a recently recognized multihost pathogen. Given that M. tb, M. …

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Metabolic and genomic adaptations of Salmonella Typhimurium grown on itaconate

Jacob Pierscianowski, Farhan Rahman Chowdhury, Andréanne Lupien, Brandon Findlay et autres

Abstract Salmonella enterica ser. Typhimurium (STm) is an enteric pathogen that causes almost 100 million cases of salmonellosis a year. A hallmark of STm is their ability to survive and replicate in the macrophages that phagocytose them. Inside the Salmonella -containing vesicles …

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Enhanced Virulence of Animal-Associated Members of the Mycobacterium Tuberculosis Complex: A Comparative Analysis in Two Hosts

Sarah N Danchuk, Shannon C. Duffy, Jaryd Sullivan, Syed Beenish Rufai et autres

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Antibiotic lethality dictates mycobacterial infection outcomes

Alexander Jovanovic, Frederick K. Bright, Ahmad Reza Sadeghi, Basil Wicki et autres

ABSTRACT Antibiotic development and treatment focus on bacterial growth inhibition, often with limited success. Here, we introduce Antimicrobial Single-Cell Testing (ASCT), an advanced imaging strategy to assess bacterial killing in real-time. By tracking 140 million bacteria and generating over 20,000 in vitro …

ch, ca, gb, au, us, de, Afrique du Sud (code pays fourni par la source)

Discovery of benzo[c]phenanthridine derivatives with potent activity against multidrug-resistant Mycobacterium tuberculosis

Yi Chu Liang, Zhiqi Sun, Lu Chen, Andréanne Lupien et autres

ABSTRACT Mycobacterium tuberculosis (Mtb), the pathogen responsible for tuberculosis (TB), is the leading cause of bacterial disease-related death worldwide. Current antibiotic regimens for the treatment of TB remain dated and suffer from long treatment times as well as the development of drug …

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Correction: Establishment of persistent enteric mycobacterial infection following streptomycin pre-treatment

Shannon C. Duffy, Andréanne Lupien, Youssef A. Elhaji, Mina S. Farag et autres

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