Andrei V. Karginov

Tuning a light-regulated allosteric switch for enhanced temporal control of protein activity

Jacob Matsche, Jordan Fauser, Trisha Bansal, Nicholas Leschinsky et autres

ABSTRACT Optogenetics enables researchers to control protein localization, interactions, and activity using photosensitive domains. The key desired properties for optogenetic tools include broad applicability, tight light-regulated control with high dynamic range, and tunability. Previously, we described an engineered light-sensitive switch, LightR, composed …

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Abstract 2415 Tuning a light-regulated allosteric switch for enhanced temporal control of enzymes

Jacob Matsche, Jordan Fauser, Trisha Bansal, Nic Leschinsky et autres

Optogenetics is a rapidly expanding field with implementation of photosensitive domains to facilitate the regulation of protein localization, interactions and activation. The key desired properties for new tools include broad applicability, tight light-regulated control with good dynamic range of regulation, and tunability …

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Abstract 2841 Development of an engineered light-regulated biotin ligase for interrogation of protein interactions with high temporal resolution

Hanna Bradford-Olson, Nic Leschinsky, Jacob Matsche, Ryuhjin Ahn et autres

Olive oil, primarily composed of triglycerides derived from oleic fatty acid, is widely recognised for its extensive health benefits.However, the creation of nanoparticles from olive oil represents a novel development in nanotechnology.This study focuses on the synthesis of carbon dot nanoparticles using …

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Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Trisha Bansal, Nicholas Lechinsky, Andrei V. Karginov

Optogenetics offers the potential for mimicking complex spatiotemporal control of enzyme activity down to a subcellular resolution. However, most optogenetic approaches often face significant challenges in integrating multiple capabilities in a single tool applicable to a wide range of target proteins. Achieving …

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Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Barbara N. Szynal, Hanna Bradford-Olson, Andrei V. Karginov

Tyrosine phosphatases are an important family of enzymes that regulate critical physiological functions. They are often dysregulated in human diseases, making them key targets of biological studies. Tools that enable the regulation of phosphatase activity are instrumental in the dissection of their …

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Parallel Activation of Src and Hif1α Increases Localized Glycolytic ATP Generation for Re-assembly of Endothelial Adherens Junctions

Li Wang, Priyanka Gajwani, Pallavi Chaturvedi, Zhigang Hong et autres

Abstract Endothelial adherens junctions (AJs) are critical for the regulation of vascular barrier integrity and undergo dis-assembly during inflammatory injury, thus causing vascular leakiness. AJ re-assembly is thus necessary for restoration of the endothelial barrier following the initial injury. Here we examine …

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Dissecting protein tyrosine phosphatase signaling by engineered chemogenetic control of its activity

Jordan Fauser, Vincent Huyot, Jacob Matsche, Barbara N. Szynal et autres

Protein tyrosine phosphatases (PTPases) are critical mediators of dynamic cell signaling. A tool capable of identifying transient signaling events downstream of PTPases is essential to understand phosphatase function on a physiological time scale. We report a broadly applicable protein engineering method for …

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Engineered Allosteric Regulation of Protein Function

Jordan Fauser, Nicholas Leschinsky, Barbara N. Szynal, Andrei V. Karginov

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Förster resonance energy transfer biosensors for fluorescence and time-gated luminescence analysis of rac1 activity

Hà Phạm, Mona Hoseini Soflaee, Andrei V. Karginov, Lawrence W. Miller

Genetically encoded, Förster resonance energy transfer (FRET) biosensors enable live-cell optical imaging of signaling molecules. Small conformational changes often limit the dynamic range of biosensors that combine fluorescent proteins (FPs) and sensing domains into a single polypeptide. To address this, we developed …

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PKCθ-mediated serine/threonine phosphorylations of FAK govern adhesion and protrusion dynamics within the lamellipodia of migrating breast cancer cells

Lucie Chadelle, Jiaying Liu, Valérie Choesmel-Cadamuro, Andrei V. Karginov et autres

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Tyrosine phosphorylation of S1PR1 leads to chaperone BiP-mediated import to the endoplasmic reticulum

Mumtaz Anwar, Md Ruhul Amin, Vijay Avin Balaji Ragunathrao, Jacob Matsche et autres

Cell surface G protein-coupled receptors (GPCRs), upon agonist binding, undergo serine-threonine phosphorylation, leading to either receptor recycling or degradation. Here, we show a new fate of GPCRs, exemplified by ER retention of sphingosine-1-phosphate receptor 1 (S1PR1). We show that S1P phosphorylates S1PR1 …

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FRET and LRET Biosensors for Cell-based Imaging and Screening of Rac1 Activation

Hà Phạm, Mona Hoseini Soflaee, Andrei V. Karginov, Lawrence W. Miller

ABSTRACT Rac1 is a key regulator of several cell signaling pathways and dysregulated Rac1 activation has been implicated in cancer. Genetically encoded Förster resonance energy transfer (FRET) biosensors with enhanced dynamic range enabled live cell fluorescence imaging of Rac1 activity and a …

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