Jaime Santos

Process Analytical technology by ultrasounds for industrial product control

Jaime Santos, João Ferreira, Mario J. Santos, Basinnov Life Sciences et autres

Process Analytical Technology (PAT) is a regulatory and scientific framework introduced by the Food and Drug Administration (FDA) to promote innovation and efficiency in pharmaceutical manufacturing, by enabling in-line, real-time process monitoring and quality control, consistent with quality by design (QbD) principles, …

Cotranslational Assembly of Oligomeric Proteins

Jaime Santos, Sander J. Tans, Nenad Ban, Günter Krämer et autres

The assembly of newly synthesized proteins into functionally active oligomers has long been regarded as a posttranslational process driven by random collision of subunits. However, growing evidence indicates that, for many proteins, assembly occurs cotranslationally, tightly coupling synthesis, folding, and subunit assembly. …

A simple, fast, and cost-efficient protocol for ultra-sensitive ribosome profiling

Jiří Koubek, Katharina Jetzinger, Shiran Dror, Mikel Irastortza‐Olaziregi et autres

Ribosome profiling has become an essential tool for studying messenger RNA (mRNA) translation in cells with codon-level resolution. However, its widespread application remains hindered by the labour-intensive workflow, low efficiency, and high costs associated with sequencing sample preparation. Here, we present a …

de, il (code pays fourni par la source)

NAC promotes co-translational protein folding at the ribosomal tunnel exit

Jaime Santos, Manuel Günnigmann, Radoslaw J. Gora, Marija Iljina et autres

The nascent polypeptide-associated complex (NAC) coordinates enzymatic modifications and membrane targeting of nascent chains during translation. While NAC's function as a dynamic hub for other factors is well-established, its direct role in co-translational folding is unclear. By proteome-wide profiling NAC co-translational interactions …

de, us, nl, ir, ch (code pays fourni par la source)

A simple, fast and cost-efficient protocol for ultra-sensitive ribosome profiling

Jiří Koubek, Katharina Jetzinger, Shiran Dror, Mikel Irastortza‐Olaziregi et autres

Abstract Ribosome profiling has become an essential tool for studying mRNA translation in cells with codon-level resolution. However, its widespread application remains hindered by the labour-intensive workflow, low efficiency and high costs associated with sequencing sample preparation. Here, we present a new …

de, il (code pays fourni par la source)

CryoEM structural characterization of α-Synuclein Oligomers in complex with PSMa3 peptide

Jaime Santos, Jorge Cuéllar, Irantzu Pallarès, José Valpuesta et autres

EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.

CryoEM structural characterization of α-Synuclein Oligomer

Jaime Santos, Jorge Cuéllar, Irantzu Pallarès, José Valpuesta et autres

EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.

A Targetable N-Terminal Motif Orchestrates α-Synuclein Oligomer-to-Fibril Conversion

Jaime Santos, Jorge Cuéllar, Irantzu Pallarès, Emily J. Byrd et autres

Oligomeric species populated during α-synuclein aggregation are considered key drivers of neurodegeneration in Parkinson's disease. However, the development of oligomer-targeting therapeutics is constrained by our limited knowledge of their structure and the molecular determinants driving their conversion to fibrils. Phenol-soluble modulin α3 …

es, gb, fr, dk (code pays fourni par la source)

An α-helical peptide-based plasmonic biosensor for highly specific detection of α-synuclein toxic oligomers

Juliana Fátima Giarola, Jaime Santos, M.‐Carmen Estévez, Salvador Ventura et autres

BACKGROUND: α-Synuclein (αS) aggregation is the main neurological hallmark of a group of neurodegenerative disorders, collectively referred to as synucleinopathies, of which Parkinson's disease (PD) is the most prevalent. αS oligomers are elevated in the cerebrospinal fluid (CSF) of PD patients, standing …

es (code pays fourni par la source)

A glimpse into the structural properties of α‐synuclein oligomers

Jaime Santos, Irantzu Pallarès, Salvador Ventura

α-Synuclein (αS) aggregation is the main neurological hallmark of a group of debilitating neurodegenerative disorders, collectively referred to as synucleinopathies, of which Parkinson's disease is the most prevalent. αS oligomers formed during the initial stages of aggregation are considered key pathogenic drivers …

es (code pays fourni par la source)

DisProt in 2024: improving function annotation of intrinsically disordered proteins

Maria Cristina Aspromonte, María Victoria Nugnes, Federica Quaglia, Vasileios Sagris et autres

DisProt (URL: https://disprot.org) is the gold standard database for intrinsically disordered proteins and regions, providing valuable information about their functions. The latest version of DisProt brings significant advancements, including a broader representation of functions and an enhanced curation process. These improvements aim …

it, hu, de, fr, es, cy (code pays fourni par la source)

Arabidopsismetacaspase MC1 localizes in stress granules, clears protein aggregates, and delays senescence

Nerea Ruiz-Solaní, Jose Salguero Linares, Laia Armengot, Jaime Santos et autres

Stress granules (SGs) are highly conserved cytoplasmic condensates that assemble in response to stress and contribute to maintaining protein homeostasis. These membraneless organelles are dynamic, disassembling once the stress is no longer present. Persistence of SGs due to mutations or chronic stress …

es, si, de, se (code pays fourni par la source)