Jodie R. Malcolm

Open Hardware: STL Files for Custom 3D-Printed OpenFlexure Microscope and Raspberry Pi Display Holder

Mark Bentley, Jodie R. Malcolm

Live-cell timelapse imaging is transforming how researchers address critical health challenges, including cancer, Alzheimer’s disease, and neglected tropical diseases (NTDs). However, advanced imaging systems capable of high-resolution timelapse acquisition are often prohibitively expensive, particularly for researchers in low- to middle-income countries (LMICs). …

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Author Reply to Peer Reviews of An ERα-Dependent Hypoxia Response Defines EMT-Adjacent Tumour Regions and Suppresses the Pro-survival Effects of Amiloride in Estrogen Receptor-Positive Breast Cancer

Jodie R. Malcolm, Jack Stenning, Jacob Pope, Jakub Lukaszonek et autres

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Adapting the OpenFlexure Microscope for Affordable Live-Cell Imaging

Jodie R. Malcolm, Olympia Physouni, Lola K Kelly, Juliana B T Carnielli et autres

Abstract Live-cell imaging (LCI) captures dynamic cellular behaviours missed in fixed samples. Researchers in resource-limited settings are underrepresented in the adoption of LCI due to the prohibitive cost of dedicated systems. We adapted the 3D-printed OpenFlexure Microscope (OFM) for LCI in humid …

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An ERα-Dependent Hypoxia Response Defines EMT-Adjacent Tumour Regions and Suppresses the Pro-survival Effects of Amiloride in Estrogen Receptor-Positive Breast Cancer

Jodie R. Malcolm, Jack Stenning, Jakub Lukaszonek, Susanna F Rose et autres

Abstract Estrogen receptor-positive (ER+) breast cancer carries a lifelong risk of recurrence and disease progression, with hypoxia-associated transcriptional signatures linked to poor prognosis and therapy resistance. While the effects of hypoxia on tumour progression are well studied, the impact on ERα epigenomic …

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Characterising cancer-stroma interactions through high-content phenotyping from microscopy time-lapses

L. F. Wiggins, Jodie R. Malcolm, Karen Hogg, Peter O’Toole et autres

Abstract Understanding how cancer-stromal interactions shape cancer progression requires tools that can capture dynamic phenotypic changes in physiologically relevant conditions. Traditional approaches for studying co-culture interactions, such as transcriptomics and flow cytometry, provide valuable insights but are limited by their static nature …

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Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell lines

Jodie R. Malcolm, Katherine S. Bridge, Andrew N. Holding, William J. Brackenbury

Hypoxia is common in breast tumours and is linked to therapy resistance and advanced disease. To understand hypoxia-driven breast cancer progression, RT-qPCR is a widely used technique to quantify transcriptional changes that occur during malignant transformation. Reference genes (RGs) are endogenous RT-qPCR …

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Hypoxia-induced epigenetic regulation of breast cancer progression and the tumour microenvironment

Alina L. Capatina, Jodie R. Malcolm, Jack Stenning, Rachael L. Moore et autres

in mediating hypoxic adaptation via chromatin remodelling, transcription factor recruitment and changes in signalling pathways; and we briefly summarise some of the findings regarding vesicle secretion and paracrine induced epigenetic reprogramming upon hypoxic exposure in breast cancer. By summarising these observations, this …

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Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell lines

Jodie R. Malcolm, Katherine S. Bridge, Andrew N. Holding, William J. Brackenbury

Abstract Hypoxia is common in breast tumours and is linked to therapy resistance and advanced disease. To understand hypoxia-driven breast cancer progression, RT-qPCR quantifies transcriptional changes important for malignant development. Reference genes (RGs) are endogenous RT-qPCR controls used to normalise mRNA levels, …

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Voltage-gated sodium channels, sodium transport and progression of solid tumours

Jodie R. Malcolm, Nattanan Sajjaboontawee, Şerife Yerlikaya, Charlotte Plunkett-Jones et autres

) in malignant cells, which influences the progression of solid tumours, predominantly driving cancer cells towards a more aggressive and metastatic phenotype. Conversely, re-expression of VGSC β subunits in cancer cells can either enhance tumour progression or promote anti-tumourigenic properties. Metastasis is …

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Widespread association of ERα with RMRP and tRNA genes in MCF-7 cells and breast cancers

Jodie R. Malcolm, Natasha K. Leese, Philippa I. Lamond-Warner, William J. Brackenbury et autres

tRNA gene transcription by RNA polymerase III (Pol III) is a tightly regulated process, but dysregulated Pol III transcription is widely observed in cancers. Approximately 75% of all breast cancers are positive for expression of Estrogen Receptor alpha (ERα), which acts as …

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Alternative isoforms of RNA polymerase III impact the non-coding RNA transcriptome, viability, proliferation and differentiation of prostate cancer cells

Jodie R. Malcolm, Robert J. White

RNA polymerase III (pol III) synthesizes short noncoding RNA (ncRNA) exclusively and is unique in having alternative paralogues of one of its subunits, POLR3G and POLR3GL. Although most pol III target loci can be transcribed by either isoform, exceptions have been found. …

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Abstract 2872: Methods of assessment of polysialyltransferase inhibitors for treatment of tumor cell dissemination

Xiaoxiao Guo, Jodie R. Malcolm, Anjana Patel, Marrwa M. Ali et autres

Abstract Polysialic acid (polySia) decorates the surface of NCAM (neuronal cell adhesion molecule) on neuroendocrine tumors, notably neuroblastoma and small cell lung cancer, and is strongly associated with poor prognosis and aggressive disease in patients in the clinic [1]. PolySia modulates tumor …

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