E. Winkelmann

Analyses of mutations selected by passaging a chimeric flavivirus identify mutations that alter infectivity and reveal an interaction between the structural proteins and the nonstructural glycoprotein NS1

Douglas G. Widman, Peter G. Mason, Ryosuke Suzuki, E. Winkelmann

We previously described a single-cycle dengue vaccine (RepliVAX D2) engineered from a capsid (C) gene-deleted West Nile virus (WNV) expressing dengue virus serotype 2 (DENV2) prM/E genes in place of the corresponding WNV genes. That work demonstrated that adaptation of RepliVAX D2 …

us (code pays fourni par la source)

Peli1 signaling blockade attenuates congenital zika syndrome

Guangyu Li, Binbin Wang, Bing Tian, Junling Gao et autres

Zika virus (ZIKV) infects pregnant women and causes devastating congenital zika syndrome (CZS). How the virus is vertically transmitted to the fetus and induces neuronal loss remains unclear. We previously reported that Pellino (Peli)1, an E3 ubiquitin ligase, promotes p38MAPK activation in …

us (code pays fourni par la source)

MAVS Is Essential for Primary CD4 + T Cell Immunity but Not for Recall T Cell Responses following an Attenuated West Nile Virus Infection

Huanle Luo, E. Winkelmann, Alan D.T. Barrett, Tian Wang et autres

ABSTRACT The use of pathogen recognition receptor (PRR) agonists and the molecular mechanisms involved have been the major focus of research in individual vaccine development. West Nile virus (WNV) nonstructural (NS) 4B-P38G mutant has several features for an ideal vaccine candidate, including …

us (code pays fourni par la source)

An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses

Guangyu Li, Awadalkareem Adam, Huanle Luo, Chao Shan et autres

Abstract Live attenuated vaccines (LAVs) are one of the most important strategies to control flavivirus diseases. The flavivirus nonstructural (NS) 4B proteins are a critical component of both the virus replication complex and evasion of host innate immunity. Here we have used …

us (code pays fourni par la source)

A Structure-Informed Atlas of Human-Virus Interactions

Gorka Lasso, Sandra Vanderli Mayer, E. Winkelmann, Tim Chu et autres

us (code pays fourni par la source)

A Structure Informed Atlas of Pan-Viral Interactions Reveals Features of Human Infection

Gorka Lasso, Sandra Vanderli Mayer, E. Winkelmann, Tim Chu et autres

us (code pays fourni par la source)

Peli1 facilitates virus replication and promotes neuroinflammation during West Nile virus infection

Huanle Luo, E. Winkelmann, Shuang Zhu, Wenjuan Ru et autres

The E3 ubiquitin ligase Pellino 1 (Peli1) is a microglia-specific mediator of autoimmune encephalomyelitis. Its role in neurotropic flavivirus infection is largely unknown. Here, we report that mice deficient in Peli1 (Peli1-/-) were more resistant to lethal West Nile virus (WNV) infection …

it, us (code pays fourni par la source)

Zika, dengue and yellow fever viruses induce differential anti-viral immune responses in human monocytic and first trimester trophoblast cells

Huanle Luo, E. Winkelmann, Ildefonso Fernández‐Salas, Li Li et autres

Abstract Zika virus (ZIKV) is a mosquito-borne flavivirus associated with severe neonatal birth defects, but the causative mechanism is incompletely understood. ZIKV shares sequence homology and early clinical manifestations with yellow fever virus (YFV) and dengue virus (DENV) and are all transmitted …

us, pe, mx (code pays fourni par la source)

Zika, dengue and yellow fever viruses induce differential anti-viral immune responses in human monocytic and first trimester trophoblast cells

Huanle Luo, E. Winkelmann, Ildefonso Fernández‐Salas, Li Li et autres

us, mx (code pays fourni par la source)

p38MAPK plays a critical role in induction of a pro-inflammatory phenotype of retinal Müller cells following Zika virus infection

Shuang Zhu, Huanle Luo, Hua Liu, Yonju Ha et autres

us (code pays fourni par la source)

MAVS Is Essential for Primary CD4+T Cell Immunity but Not for Recall T Cell Responses following an Attenuated West Nile Virus Infection

Huanle Luo, E. Winkelmann, Guorui Xie, Rong Fang et autres

ABSTRACT The use of pathogen recognition receptor (PRR) agonists and the molecular mechanisms involved have been the major focus of research in individual vaccine development. West Nile virus (WNV) nonstructural (NS) 4B-P38G mutant has several features for an ideal vaccine candidate, including …

us (code pays fourni par la source)

West Nile Virus Infection in the Central Nervous System

E. Winkelmann, Huanle Luo, Tian Wang

West Nile virus (WNV), a neurotropic single-stranded flavivirus has been the leading cause of arboviral encephalitis worldwide. Up to 50% of WNV convalescent patients in the United States were reported to have long-term neurological sequelae. Neither antiviral drugs nor vaccines are available …

us (code pays fourni par la source)