Fetal liver macrophages contribute to the hematopoietic stem cell niche by controlling granulopoiesis
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Le résumé fourni par la source
Abstract During embryogenesis, the fetal liver becomes the main hematopoietic organ, where stem and progenitor cells as well as immature and mature immune cells form an intricate cellular network. Hematopoietic stem cells (HSCs) reside in a specialized niche, which is essential for their proliferation and differentiation. However, the cellular and molecular determinants contributing to this fetal HSC niche remain largely unknown. Macrophages are the first differentiated hematopoietic cells found in the developing liver, where they are important for fetal erythropoiesis by promoting erythrocyte maturation and phagocytosing expelled nuclei. Yet, whether macrophages play a role in fetal hematopoiesis beyond serving as a niche for maturing erythroblasts remains elusive. Here, we investigate the heterogeneity of macrophage populations in the fetal liver to define their specific roles during hematopoiesis. Using a single-cell omics approach combined with spatial proteomics and genetic fate-mapping models, we found that fetal liver macrophages cluster into distinct yolk sac-derived subpopulations and that long-term HSCs are interacting preferentially with one of the macrophage subpopulations. Fetal livers lacking macrophages show a delay in erythropoiesis and have an increased number of granulocytes, which can be attributed to transcriptional reprogramming and altered differentiation potential of long-term HSCs. Together, our data provide a detailed map of fetal liver macrophage subpopulations and implicate macrophages as part of the fetal HSC niche.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Fetal liver macrophages contribute to the hematopoietic stem cell niche by controlling granulopoiesis
- Date Crossref
- 23/02/2023
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Bonn Life & Medical Sciences (LIMES) Institute pays non établi dans la noticeUniversité ou école supérieure
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Quantitative BioSciences pays non établi dans la noticeOrganisation à but non lucratif
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German Center for Neurodegenerative Diseases pays non établi dans la noticeStructure de recherche
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Universitätsklinikum Erlangen Department of Internal Medicine 3-Rheumatology and Immunology pays non établi dans la noticeÉtablissement de santé
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Life & Brain (Germany) pays non établi dans la noticeEntreprise
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Immunogenomics & Neurodegeneration pays non établi dans la noticeInstitution
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Systems Medicine pays non établi dans la noticeInstitution
Life & Medical Sciences (LIMES) Institute — University of Bonn, Quantitative BioSciences et German Center for Neurodegenerative Diseases, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.