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Accès ouvert déclaré 2020 article

Robust estimation of bacterial cell count from optical density

245Citations signalées — pas une note de qualité
224Institutions déclarées
34Pays d’affiliation déclarés

Résumé fourni par la source

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.

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Contrôle bibliographique ouvert

DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.

Titre Crossref
Robust estimation of bacterial cell count from optical density
Date Crossref
17/09/2020
Éditeur
Springer Science and Business Media LLC
Type
journal-article

Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude et ne compte pas comme une seconde source scientifique indépendante.

Institutions déclarées

RTX (United States)Worcester Polytechnic InstituteIGlobal UniversityImperial College LondonSynthace (United Kingdom)Waseda UniversityCalifornia Institute of TechnologyTechnical University of DenmarkRWTH Aachen UniversityÉcole Polytechnique Fédérale de LausanneUniversity of HelsinkiAalto UniversityAnhui University of TechnologyNanjing UniversityShanghai Jiao Tong UniversityNortheastern UniversitySouth China University of TechnologyStony Brook UniversitySouth China Agricultural UniversityAix-Marseille UniversitéBGI Group (China)Fudan UniversityZhejiang Party SchoolChina Pharmaceutical UniversityStony Brook SchoolNanjing Foreign Language SchoolNanjing Agricultural UniversityNational Technical University of AthensAthens University of Economics and BusinessUniversity of British ColumbiaThe University of Texas at AustinSpace Telescope Science InstituteBeijing City UniversityBeijing Foreign Studies UniversityUNSW SydneyBen-Gurion University of the NegevNorthwestern Polytechnical UniversityChinese University of Hong KongNational Tsing Hua UniversityJiangnan UniversityUniversity of Science and Technology of ChinaBielefeld UniversityHubei University of TechnologyHubei UniversityBilkent UniversityInstitut Químic de SarriàCHA UniversityBeijing Institute of TechnologyGeorgia State UniversityUniversity of WashingtonBeijing Normal UniversityGuangzhou Experimental StationNational Chung Cheng UniversityUniversity of CalgaryDalian University of TechnologyOcean University of ChinaCardiff UniversityShanghai International Studies UniversityShanghaiTech UniversityChengdu UniversityChalmers University of TechnologyCarnegie Mellon University QatarCarnegie Mellon UniversityNational Yang Ming Chiao Tung UniversityNorwegian University of Science and TechnologyNational Yang Ming Chiao Tung UniversityMingDao UniversityColorado School of MinesWilliam & MaryWilliams (United States)Columbia UniversityUniversity of Electronic Science and Technology of ChinaCornell UniversityCentral South UniversityColorado State UniversityUniversity of Science and Technology BeijingHeinrich Heine University DüsseldorfUniversidad de las Fuerzas Armadas ESPEEast China University of Science and TechnologyUniversity of EdinburghEmory UniversityUniversity of TorontoETH ZurichZhejiang University of TechnologyUniversité Paris-SaclayUniversity of ExeterFriedrich-Alexander-Universität Erlangen-NürnbergFujian Normal UniversityGifu UniversityUniversity of GroningenHankuk University of Foreign StudiesHebrew University of JerusalemIndian Institute of Science BangaloreUniversity of Hong KongHong Kong Chu Hai CollegeSt. Paul's Co-educational CollegeHong Kong University of Science and TechnologyUnited Christian HospitalIndian Institute of Science Education and Research KolkataHuazhong University of Science and TechnologyHuazhong Agricultural UniversityInstitute of Chemical TechnologySichuan UniversityIndian Institute of Science Education and Research, BhopalIndian Institute of Science Education and Research MohaliIndian Institute of Technology DelhiIndian Institute of Technology KanpurIndian Institute of Technology MadrasJilin UniversityUniversity of WürzburgKanagawa Institute of TechnologySun Yat-sen UniversityNational Chung Hsing UniversityKing's College LondonKorea UniversityLeiden UniversityUniversity of WarwickUniversity of LethbridgeChinook Regional HospitalLethbridge CollegeTexas Tech UniversityLund UniversityKTH Royal Institute of TechnologyMacquarie UniversityUniversidad Complutense de MadridUniversidad Carlos III de MadridUniversity of ManchesterPhilipps University of MarburgMcGill UniversityMcMaster UniversityUniversity of Illinois Urbana-ChampaignUniversity of MichiganMichigan State UniversityUniversity of MinnesotaUniversité de MontpellierLMU KlinikumTechnical University of MunichLudwig-Maximilians-Universität MünchenGraz University of TechnologyNawi GrazNewcastle UniversityNankai UniversityNorthwestern UniversityUniversity of NottinghamNanyang Technological UniversityNazarbayev UniversityNational University of Defense TechnologyNational University of SingaporeAction NetworkNorthwest UniversityUniversité Sorbonne NouvelleSorbonne UniversitéUniversity of OxfordInstitut PasteurSecond Military Medical UniversityPeking UniversityUniversity of PittsburghPurdue University West LafayetteQueen's UniversityRajalakshmi Engineering CollegeRose–Hulman Institute of TechnologyRice UniversityShandong UniversityShenzhen Baoan High School GroupShenzhen Institute of Information TechnologyUniversity of St AndrewsStanford UniversityRhode Island School of DesignProvidence CollegeBrown UniversityUniversity College of Arts Crafts and DesignKarolinska InstitutetUniversity of StuttgartUniversity of Illinois ChicagoUniversity of ChicagoShenzhen UniversityUniversity of TartuZhejiang UniversityTecnológico de MonterreyXiamen UniversityAristotle University of ThessalonikiTongji UniversityUniversité Toulouse III - Paul SabatierInstitut National des Sciences Appliquées de ToulouseTechnische Universität DarmstadtEindhoven University of TechnologyDelft University of TechnologyTianjin University of Science and TechnologyUniversity of AlbertaUniversity of California, DavisUniversity of California San DiegoUniversity of Chinese Academy of SciencesUniversity of ChileUniversity College LondonUCLouvainUniversity of ConnecticutUniversity of CopenhagenUniversity of FloridaUniversity of GeorgiaUniversity of IndonesiaUniversity of OsloUniversity of IowaUniversité LavalUniversity of La VerneUniversity of Maryland, College ParkUniversity of Nebraska–LincolnUniversidade Estadual Paulista (Unesp)Universitat Pompeu FabraCentre for Genomic RegulationUppsala UniversityUnited States Military AcademyUniversidade de São PauloUniversity of Tennessee at KnoxvilleUtrecht UniversityUniversitat Politècnica de ValènciaVilnius UniversityUniversity of VirginiaWashington University in St. LouisUniversity of WaterlooUniversity of WestminsterWuhan UniversityXi’an Jiaotong-Liverpool UniversityXi'an Jiaotong UniversityYale University

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