Yuki Ono

Chemogenetic activation of hepatic G12 signaling ameliorates hepatic steatosis and obesity

Kaito Arai, Yuki Ono, Yuki Sugiura, Keizo Kaneko et autres

Objective Hepatic steatosis , the early stage of nonalcoholic fatty liver disease (NAFLD), currently lacks targeted pharmacological treatments. G protein-coupled receptors (GPCRs) in hepatocytes differentially regulate lipid metabolism depending on their coupling profile of G protein subtypes. Unlike G s , G …

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Discovery of an antimalarial compound, burnettiene A, with a multidrug-sensitive Saccharomyces cerevisiae screening system based on mitochondrial function inhibitory activity

Aoi Kimishima, Atsuka Nishitomi, Iori Tsuruoka, Katsuyuki Sakai et autres

In this paper, we describe our discovery of burnettiene A (1) as an antimalarial compound from the culture broth of Lecanicillium primulinum (current name: Flavocillium primulinum) FKI-6715 strain utilizing our original multidrug-sensitive yeast system. This polyene-decalin polyketide natural product was originally isolated …

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Skeletal Muscle Mass Index Reduction Rate as a Prognostic Indicator for Patients Undergoing Pancreatectomy for Pancreatic Cancer

Junya Mita, Takashi Maeda, Eiji Tsujita, Naotaka Hashimoto et autres

Background/Aim: There have been many studies on skeletal muscle depletion before surgery, and skeletal muscle depletion is a known risk factor for poor prognosis. However, reports on the association between changes in skeletal muscle mass and prognosis after surgery for pancreatic cancer …

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Cost-Effectiveness Analysis of FOLFIRI-Based First-Line Regimens for Metastatic Colorectal Cancer Using Clinical Decision Analysis.

Toshihiro Shida, Yuki Ono, Yuka Kobayashi, Jiro Ogura et autres

OBJECTIVE: Metastatic colorectal cancer with KRAS wild type is treated using a range of drug regimens, including fluorouracil, irinotecan, and Leucovorin(FOLFIRI)plus bevacizumab(Bmab), cetuximab(Cmab), or panitumumab(Pmab). The present study aimed to identify the optimal regimen using a decision analysis method, in combination with …

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A case of difficult-to-diagnose hepatic reactive lymphoid hyperplasia finally diagnosed by using PCR analysis of IgH-gene rearrangements: a case report

Junya Mita, Takashi Maeda, Eiji Tsujita, Kyohei Yugawa et autres

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Chemogenetic activation of G12 signaling enhances adipose tissue browning

Yuki Ono, Ryo Ito, Kaito Arai, Gurdeep Singh et autres

Beige adipocytes, which increase energy expenditure by dissipating energy as heat, have gained attention as a therapeutic target for combating obesity. 1 Adipocytes express many types of G-protein-coupled receptors (GPCRs), each of which has a unique preference for the G s , …

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Generation of Gαi knock-out HEK293 cells illuminates Gαi-coupling diversity of GPCRs

Yuki Ono, Kouki Kawakami, Gaku Nakamura, Satoru Ishida et autres

G-protein-coupled receptors (GPCRs) are pivotal cell membrane proteins that sense extracellular molecules and activate cellular responses. The G-protein α subunit i (Gαi) family represents the most common GPCR-coupling partner and consists of eight subunits with distinct signaling properties. However, analyzing the coupling …

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Ectodomain shedding of EGFR ligands serves as an activation readout for TRP channels

Manae Tatsumi, Takayuki Kishi, Satoru Ishida, Hiroki Kawana et autres

Transient receptor potential (TRP) channels are activated by various extracellular and intracellular stimuli and are involved in many physiological events. Because compounds that act on TRP channels are potential candidates for therapeutic agents, a simple method for evaluating TRP channel activation is …

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An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA , microtubules, and microfilaments

Motonari Okabe, Hiromitsu Shirasawa, Yuki Ono, Mayumi Goto et autres

Purpose: Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of …

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OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz

Alastair C. Keen, Laura Lemel, Daniel J. Scott, Meritxell Canals et autres

Abstract Heterotrimeric G proteins are the main signalling effectors for G protein-coupled receptors. Understanding the distinct functions of different G proteins is key to understanding how their signalling modulates physiological responses. Pertussis toxin, a bacterial AB5 toxin, inhibits Gαi/o G proteins and …

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Heterotrimeric Gq proteins act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias

Kouki Kawakami, Masataka Yanagawa, Suzune Hiratsuka, Misaki Yoshida et autres

Signaling-biased ligands acting on G-protein-coupled receptors (GPCRs) differentially activate heterotrimeric G proteins and β-arrestins. Although a wealth of structural knowledge about signaling bias at the GPCR level exists (preferential engagement of a specific transducer), little is known about the bias at the …

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Heterotrimeric G Protein Subunit Gαq Is a Master Switch for Gβγ-Mediated Calcium Mobilization by Gi-Coupled GPCRs

Eva Marie Pfeil, Julian Brands, Nicole Merten, Timo Vögtle et autres

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