Yexuan Deng

A high-density CRISPR activation platform for mapping cancer dependencies and resistance pathways ex vivo and in vivo

Sarah T. Diepstraten, Yexuan Deng, Margaret A. Potts, Amy J. Heidersbach et autres

CRISPR activation (CRISPRa) enables precise up-regulation of gene expression for ex vivo and in vivo applications. However, a lack of scalable, high-coverage tools has limited comprehensive genetic screening in murine models. Here, we introduce Partita, a next-generation mouse whole-genome CRISPRa sgRNA platform, …

au, cn (code pays fourni par la source)

3107 – ADVANCED CRISPR KNOCKOUT AND ACTIVATION MOUSE MODELS AS TOOLS FOR INVESTIGATING HEMATOLOGY AND HEMATOLOGICAL MALIGNANCIES

John E. La Marca, Sarah T. Diepstraten, Wei Jin, Yexuan Deng et autres

au (code pays fourni par la source)

RIPPLY1 suppresses cancer cell stemness via targeting TBX19 in CTNNB1-mutated hepatocellular carcinoma

Guangyan Zhangyuan, Weiwei Yu, Wenfang Tian, Jun Wang et autres

BACKGROUND AND AIMS: CTNNB1 -mutated HCCs exhibit a relatively low stem-like and well-differentiated phenotype. However, the mechanism remains unclear. Ripply transcriptional repressor 1 (RIPPLY1), a transcriptional repressor required for somite segmentation, has hardly been studied in cancer. Here, we aim to unveil …

cn (code pays fourni par la source)

Genome-wide in vivo CRISPR screens identify GATOR1 complex as a tumor suppressor in Myc-driven lymphoma

Margaret A. Potts, Shinsuke Mizutani, Yexuan Deng, Srimayee Vaidyanathan et autres

Identifying tumor suppressor genes is predicted to inform on the development of novel strategies for cancer therapy. To identify new lymphoma driving processes that cooperate with oncogenic MYC, which is abnormally highly expressed in ~70% of human cancers, we use a genome-wide …

au, jp, cn, sg, us (code pays fourni par la source)

A novel, high-density CRISPR activation platform for mapping cancer dependencies and resistance pathways ex vivo and in vivo

Sarah T. Diepstraten, Yexuan Deng, Margaret A. Potts, Amy J. Heidersbach et autres

Abstract CRISPR activation (CRISPRa) enables precise, locus-specific upregulation of gene expression, offering potential for both ex vivo and in vivo applications. However, the lack of scalable, high-coverage tools has limited its use in comprehensive genetic screens, particularly in murine models. Here, we …

au, cn, ca (code pays fourni par la source)

Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening

Wei Jin, Yexuan Deng, John E. La Marca, Emily J. Lelliott et autres

Cas12a is a next-generation gene editing tool that enables multiplexed gene targeting. Here, we present a mouse model that constitutively expresses enhanced Acidaminococcus sp. Cas12a (enAsCas12a) linked to an mCherry fluorescent reporter. We demonstrate efficient single and multiplexed gene editing in vitro, …

au, cn, ca (code pays fourni par la source)

Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening

Wei Jin, Yexuan Deng, John E. La Marca, Emily J. Lelliott et autres

Abstract Cas12a is a gene-editing tool that simplifies multiplexed gene targeting through its RNase activity, enabling maturation of individual crRNAs from a pre-crRNA-encoding RNA. Here, we present a mouse model that constitutively expresses enhanced Acidaminococcus sp. Cas12a ( enAsCas12a ) linked to …

au, cn (code pays fourni par la source)

Caspase‐8‐driven apoptotic and pyroptotic crosstalk causes cell death and IL‐1β release in X‐linked inhibitor of apoptosis (XIAP) deficiency

Sebastian A. Hughes, Meng Lin, Ashley L. Weir, Bing Huang et autres

Genetic lesions in X-linked inhibitor of apoptosis (XIAP) pre-dispose humans to cell death-associated inflammatory diseases, although the underlying mechanisms remain unclear. Here, we report that two patients with XIAP deficiency-associated inflammatory bowel disease display increased inflammatory IL-1β maturation as well as cell …

au, cn (code pays fourni par la source)

3155 – GENOME WIDE CRISPR/CAS9 KNOCKOUT SCREEN IN VIVO IDENTIFY GATOR1 COMPONENTS AS TUMOR SUPPRESSORS IN Eµ-MYC LYMPHOMAGENESIS

Maggie Potts, Shinsuke Mizutani, Yexuan Deng, Göknur Giner et autres

Author Correction: Generation of a CRISPR activation mouse that enables modelling of aggressive lymphoma and interrogation of venetoclax resistance

Yexuan Deng, Sarah T. Diepstraten, Margaret A. Potts, Göknur Giner et autres

In this article the affiliation details for Amy Heidersbach were incorrectly given as ‘School of Dentistry and Medical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia’ but should have been ‘Department of Molecular Biology, Genentech, Inc., South San Francisco, CA, USA’. The …

au, cn (code pays fourni par la source)

Generation of a CRISPR activation mouse that enables modelling of aggressive lymphoma and interrogation of venetoclax resistance

Yexuan Deng, Sarah T. Diepstraten, Margaret A. Potts, Göknur Giner et autres

Abstract CRISPR technologies have advanced cancer modelling in mice, but CRISPR activation (CRISPRa) methods have not been exploited in this context. We establish a CRISPRa mouse ( dCas9a-SAM KI ) for inducing gene expression in vivo and in vitro. Using dCas9a-SAM KI …

au, cn (code pays fourni par la source)

Manipulating Offense and Defense Signaling to Fight Cold Tumors with Carrier‐Free Nanoassembly of Fluorinated Prodrug and siRNA

Pengkai Wu, Haitian Zhang, Meiling Sun, Shuai Mao et autres

Abstract Chemoimmunotherapy has shown great potential to activate an immune response, but the immunosuppressive microenvironment associated with T cell exhaustion remains a challenge in cancer therapy. The proper immune‐modulatory strategy to provoke a robust immune response is to simultaneously regulate T‐cell exhaustion …

cn (code pays fourni par la source)