Li Li

Figure S8 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Figure S8 (associated with Figure 8). Increased GBAF remodeling in SyS increases H2AK119ub-associated transcription while paradoxically decreasing H2AK119ub enrichment. A. RNA-seq defined differential expression transcriptome wide following exposure of the ASKA, SYO-1, and HS-SY-II SyS cell lines to WA-68 versus DMSO …

Table S1 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Table S1

Figure S3 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Figure S3 (associated with Figure 3). WA-68 is variably efficacious against SyS in vivo. A. Necropsy CDX harvest photos from treated MoJo xenografts (mm scaled markings included). B. Harvest photos from treated HS-SY-II CDXs (mm scaled markings included). C. Harvest photos …

Figure S1 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Figure S1 (associated with Figure 1). BRD9 depletion arrests proliferation in SyS. A. Western blot indicating depletion efficiency for BRD9 by four doxycycline inducible shRNAs in the human 12733/99 SyS cell line. B. Cell viability after 12 days in culture for …

Figure S2 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Figure S2 (associated with Figure 2). WA-68 targets BRD9 in cells, molecular structure and administered in vivo. A. Median ± standard deviation for fold-change abundance of each protein calculated from mass spectrometry proteomics of WA-68 versus DMSO control for a sample …

Figure S6 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Figure S6 (associated with Figure 6). BRD9 depletion reduces GBAF and fusion oncoprotein enrichment without major changes to chromatin distribution in SyS. A. Heatmap for Pearson correlation coefficients for SS18::SSX, H2AK119ub, GLTSCR1, and other histone post-translational modifications ChIP-seq for individual tumors …

Data from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Abstract Synovial sarcoma incorporates the SS18::SSX fusion oncoprotein into GLTSCR1-containing BRG1/BRM and associated factors (GBAF) complexes, which confers a dependency on the GBAF subunit BRD9. However, synovial sarcoma clinical trials with multiple BRD9 degraders failed to achieve clinically impactful remissions. In this …

Figure S5 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Figure S5 (associated with Figure 5). BRD9 depletion does not compromise GBAF assembly. A. Western blots of the middle-fractions of the glycerol gradient fractionated nuclear extracts from HS-SY-II cells exposed to DMSO or WA-68 in vitro. B. Western blots of the …

Figure S7 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Figure S7 (associated with Figure 7). GBAF remodeler sliding activity increases in the absence of BRD9. A. RNA-seq defined differential expression comparing WA-68 exposure to control DMSO in a control set of maintained active transcription (MAT, expressed genes without fusion and …

Figure S4 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Supplemental Figure S4 (associated with Figure 4). BRD9 degradation reduces PBAF and recovers CBAF in SyS. A. Glycerol gradients fractionated nuclear extracts, blotted for a variety of BAF component antibodies after DMSO control or WA-68 exposure for HS-SY-II cells. Green lines indicate …

BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

Jinxiu Li, Mary L. Nelson, Li Li, Xiaobo Xia et autres

Synovial sarcoma incorporates the SS18::SSX fusion oncoprotein into GLTSCR1-containing BRG1/BRM and associated factors (GBAF) complexes, which confers a dependency on the GBAF subunit BRD9. However, synovial sarcoma clinical trials with multiple BRD9 degraders failed to achieve clinically impactful remissions. In this study, …

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Profiling potential targets of anlotinib in human synovial sarcoma patients and immunocompetent mouse models

Lara Carroll, Kyllie Smith-Fry, Rahmon Kanmodi, Steve Olsen et autres

ABSTRACT Synovial sarcoma (SS) is an aggressive translocation-driven malignancy characterized by its driving SS18::SSX fusion oncogene. This rare but deadly cancer presents limited treatment options despite an emerging understanding of its dependency on receptor tyrosine kinases (RTKs). Anlotinib is a multi-target tyrosine …

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