Figure S5 from BRD9 Degraders Unleash GBAF Chromatin Remodeling Activity in Synovial Sarcoma

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Supplemental Figure S5 (associated with Figure 5). BRD9 depletion does not compromise GBAF assembly. A. Western blots of the middle-fractions of the glycerol gradient fractionated nuclear extracts from HS-SY-II cells exposed to DMSO or WA-68 in vitro. B. Western blots of the middle-fractions of the glycerol gradient fractionated nuclear extracts from STG048 PDXs exposed to vehicle control saline or WA-68 in vivo. C. Western blots of transfected BioID2 with a V5 tag fused either to the carboxy or amino terminus of BRD9 in the HD-MY-Z cell line, demonstrating doxycycline induction of expression of the fused proteins. D. Mass spectrometry proteomics showing the fold-change (FC) relative abundance of biotinylated proteins in BRD9::BioID2::V5 vs. BioID2::V5::BRD9 transfected HD-MY-Z cells after doxycycline or control (purple dots indicate significance in both experiments.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.