Yohei Nishi

Am80, a retinoic acid receptor agonist, activates the cardiomyocyte cell cycle and enhances engraftment in the heart

Manabu Kasamoto, Shunsuke Funakoshi, Takeshi Hatani, Chikako Okubo et autres

Human induced pluripotent stem cell-derived (hiPSC) cardiomyocytes are a promising source for regenerative therapy. To realize this therapy, however, their engraftment potential after their injection into the host heart should be improved. Here, we established an efficient method to analyze the cell …

jp (code pays fourni par la source)

Elucidation of HHEX in pancreatic endoderm differentiation using a human iPSC differentiation model

Ryo Ito, Azuma Kimura, Yurie Hirose, Yu Hatano et autres

For pluripotent stem cell (PSC)-based regenerative therapy against diabetes, the differentiation efficiency to pancreatic lineage cells needs to be improved based on the mechanistic understanding of pancreatic differentiation. Here, we aimed to elucidate the molecular mechanisms underlying pancreatic endoderm differentiation by searching …

jp (code pays fourni par la source)

Screening Station, a novel laboratory automation system for physiologically relevant cell-based assays

Ichiji Namatame, Kana Ishii, Takashi Shin, Daisuke Shimojo et autres

Due to their physiological relevance, cell-based assays using human-induced pluripotent stem cell (iPSC)-derived cells are a promising in vitro pharmacological evaluation system for drug candidates. However, cell-based assays involve complex processes such as long-term culture, real-time and continuous observation of living cells, …

jp (code pays fourni par la source)

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells

Mio Iwasaki, Yuka Kawahara, Chikako Okubo, Tatsuya Yamakawa et autres

The effects of transcription factors on the maintenance and differentiation of human-induced or embryonic pluripotent stem cells (iPSCs/ESCs) have been well studied. However, the importance of posttranscriptional regulatory mechanisms, which cause the quantitative dissociation of mRNA and protein expression, has not been …

jp, us (code pays fourni par la source)

Post-transcriptionally regulated genes are essential for pluripotent stem cell survival

Mio Iwasaki, Yuka Kawahara, Tsuyoshi Tabata, Yohei Nishi et autres

Summary The effects of transcription factors on the maintenance and differentiation of pluripotent stem cells (PSCs) have been well studied. However, the importance of post-transcriptional regulatory mechanisms, which cause the quantitative dissociation of mRNA and protein expression, has not been explored in …

jp, us (code pays fourni par la source)

iPSC screening for drug repurposing identifies anti‐RNA virus agents modulating host cell susceptibility

Keiko Imamura, Yasuteru Sakurai, Takako Enami, Ran Shibukawa et autres

Human pathogenic RNA viruses are threats to public health because they are prone to escaping the human immune system through mutations of genomic RNA, thereby causing local outbreaks and global pandemics of emerging or re-emerging viral diseases. While specific therapeutics and vaccines …

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Establishment of a Robust Platform for Induced Pluripotent Stem Cell Research Using Maholo LabDroid

Miho Sasamata, Daisuke Shimojo, Hiromitsu Fuse, Yohei Nishi et autres

Induced pluripotent stem cells (iPSCs) are attractive for use in early drug discovery because they can differentiate into any cell type. Maintenance cultures and differentiation processes for iPSCs, however, require a high level of technical expertise. To overcome this problem, technological developments …

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Small molecule TCS21311 can replace BMP7 and facilitate cell proliferation in in vitro expansion culture of nephron progenitor cells

Hiraku Tsujimoto, Toshikazu Araoka, Yohei Nishi, Akira Ohta et autres

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A β1-tubulin–based megakaryocyte maturation reporter system identifies novel drugs that promote platelet production

Hideya Seo, Si Jing Chen, Kazuya Hashimoto, Hiroshi Endô et autres

Abstract During maturation, megakaryocytes (MKs) express β1-tubulin (TUBB1) and rearrange their microtubule components to enlarge, form proplatelets, and eventually release platelets. The development of a platform to identify in vitro conditions that would efficiently promote MK development could potentially enable large-scale platelet …

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A Novel Megakaryocyte-Based Screening System Identifies Compounds That Promote Megakaryocyte Maturation and Platelet Production in Vitro

Si Jing Chen, Hideya Seo, Akitsu Hotta, Yohei Nishi et autres

Abstract Platelets are anucleate cytoplasmic fragments derived from megakaryocytes (MKs) that play crucial roles in blood clotting system and innate immune response. The clinical needs of platelet transfusion are extensive and increasing, while current donor-derived platelet supply confronts challenges including limited donors, …

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Small molecule AT7867 proliferates PDX1-expressing pancreatic progenitor cells derived from human pluripotent stem cells

Azuma Kimura, Tarō Toyoda, Yohei Nishi, Makoto Nasu et autres

While pancreatic islet transplantation achieves insulin independence in type 1 diabetes (T1D) patients, its widespread application is limited by donor tissue scarcity. Pancreatic progenitor cells (PPCs) give rise to all cell types in the pancreas during development. PPCs derived from human pluripotent …

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The combinational use of CRISPR /Cas9‐based gene editing and targeted toxin technology enables efficient biallelic knockout of the α‐1,3‐galactosyltransferase gene in porcine embryonic fibroblasts

Masahiro Sato, K. Miyoshi, Yozo Nagao, Yohei Nishi et autres

BACKGROUND: The recent development of the type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has enabled genome editing of mammalian genomes including those of mice and human; however, its applicability and efficiency in the pig have not been studied in …

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