Si Jing Chen

Hypoxia-Induced Epas1-Myl9/12 Axis Shapes the Pathology of Pulmonary Hypertension

Sachiko Kuriyama, Chiaki Iwamura, Masahiro Kiuchi, Tetsutaro Nagaoka et autres

BACKGROUND: Pulmonary hypertension (PH) is a progressive cardiopulmonary disorder characterized by vascular remodeling, abnormal vasoconstriction of small lung arteries, and right heart failure. Hypoxia causes vascular damage, leading to vessel stenosis or occlusion by aberrant endothelial cells, hypertrophy of the tunica media, …

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Aging-dependent reduction of KAT7/HBO1 activity impairs imMKCL-based platelet production by promoting immune properties

Wei-Yin Qiu, Sou Nakamura, Sudip Kumar Paul, Takuya Yamamoto et autres

The master cell bank (MCB) system is essential for regenerative cell therapy. We have developed induced pluripotent stem cell (iPSC)-based immortalized megakaryocyte progenitor cell lines (imMKCLs) as an MCB for iPSC-derived platelet (iPSC-PLT) transfusion. However, imMKCLs exhibit both thrombopoietic and immune-skewed properties, …

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Bone morphogenetic protein-2-independent bone formation by human iPSC-derived megakaryocytes and platelets

Norichika Mizuki, Michiaki Mukai, Yasuhiro Shiga, Naoya Takayama et autres

Bone regeneration (BR) is an ongoing challenge in biomedical research. While recombinant human bone morphogenetic protein-2 (rhBMP-2) is available for clinical use, its side effects remain a concern. Patient-derived platelet-rich plasma has been proposed as an alternative, but its heterogeneity poses a …

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Scalable production and anti-tumor efficacy of iPSC-derived immortalized anti-CD19 CAR macrophages

Akihiro Nakajima, Sou Nakamura, Maria Alejandra Kanashiro, Sudip Kumar Paul et autres

Abstract Chimeric antigen receptor (CAR) -T cell therapy has shown remarkable efficacy in patients with relapsed or refractory mature B cell lymphoma and multiple myeloma. However, durable responses remain limited due to several factors, including antigen escape, T-cell exhaustion, and insufficient tumor …

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PS19.8: Tuning immune signaling in iPSC-derived megakaryocytes to improve platelet production.

Si Jing Chen, Naoshi Sugimoto, Naoya Takayama, Koji Eto

Introduction: Platelet transfusion is a standard treatment for thrombocytopenia but is limited by donor shortages, contamination risks, and transfusion refractoriness. Ex vivo production of platelets from induced pluripotent stem cells (iPSCs) represents a promising alternative. We previously established immortalized megakaryocyte progenitor cell …

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STAT1-mediated epigenetic regulation of LIN28A controls iPSC-derived platelet production through the let-7–RALB axis

Kazuya Hashimoto, Si Jing Chen, Kosuke Fujio, Akihiro Kayama et autres

ABSTRACT: Ex vivo platelet (PLT) production from induced pluripotent stem cells (iPSCs) represents a potential solution to address the limitations of donor-dependent PLT transfusion therapy. Although our established immortalized megakaryocyte progenitor cell lines (imMKCLs) from iPSCs enable large-scale production of functional iPSC-derived …

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Advances and Challenges in the Ex Vivo Production of Megakaryocytes and Platelets

Si Jing Chen, Koji Eto

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A let-7 microRNA-RALB axis links the immune properties of iPSC-derived megakaryocytes with platelet producibility

Si Jing Chen, Kazuya Hashimoto, Kosuke Fujio, Karin Hayashi et autres

We recently achieved the first-in-human transfusion of induced pluripotent stem cell-derived platelets (iPSC-PLTs) as an alternative to standard transfusions, which are dependent on donors and therefore variable in supply. However, heterogeneity characterized by thrombopoiesis-biased or immune-biased megakaryocytes (MKs) continues to pose a …

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Ex vivo manufacturing of platelets: beyond the first-in-human clinical trial using autologous iPSC-platelets

Si Jing Chen, Naoshi Sugimoto, Koji Eto

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A β1-tubulin–based megakaryocyte maturation reporter system identifies novel drugs that promote platelet production

Hideya Seo, Si Jing Chen, Kazuya Hashimoto, Hiroshi Endô et autres

Abstract During maturation, megakaryocytes (MKs) express β1-tubulin (TUBB1) and rearrange their microtubule components to enlarge, form proplatelets, and eventually release platelets. The development of a platform to identify in vitro conditions that would efficiently promote MK development could potentially enable large-scale platelet …

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A Novel Megakaryocyte-Based Screening System Identifies Compounds That Promote Megakaryocyte Maturation and Platelet Production in Vitro

Si Jing Chen, Hideya Seo, Akitsu Hotta, Yohei Nishi et autres

Abstract Platelets are anucleate cytoplasmic fragments derived from megakaryocytes (MKs) that play crucial roles in blood clotting system and innate immune response. The clinical needs of platelet transfusion are extensive and increasing, while current donor-derived platelet supply confronts challenges including limited donors, …

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Anti‐prediabetic effect of rose hip ( Rosa canina ) extract in spontaneously diabetic Torii rats

Si Jing Chen, Chiwa Aikawa, Risa Yoshida, Tomoaki Kawaguchi et autres

Abstract BACKGROUND Prediabetes, a high‐risk state for developing diabetes showing impaired glucose tolerance but a normal fasting blood glucose level, has an increasing prevalence worldwide. However, no study investigating the prevention of impaired glucose tolerance at the prediabetic stage by anti‐diabetic functional …

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