Ichiji Namatame

End-to-end automation of organ-on-a-chip culture and drug testing: Enhancing scalability and expandability

Mayu Shibuta, Kazuhiro Tetsuka, Tetsuhiro Kawabe, Ichiji Namatame et autres

The increasing demand for effective and translatable culture models in drug discovery has driven the development of advanced systems, including organ-on-a-chip (OoC), microphysiological systems (MPS), and complex in vitro models (CIVM). These technologies are recognized for their ability to model human physiological …

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Continuous evolving humanoid for advanced cellular models

Daisuke Shimojo, S. Qiu, Miho Sasamata, Ichiji Namatame et autres

Drug discovery research faces significant challenges, with around 90 % of candidate drugs failing in clinical trials. Traditional animal models do not always accurately reflect human physiology and pathology, leading to low predictability of clinical outcomes. In vitro human model using induced …

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Integration of a fully automated flow cytometry system with high robustness into a Screening Station

Shingo Fujiyama, H. Asano, Ichiji Namatame

Jurkat cells, and the lower limit of detection, linearity, and CV% were evaluated. Average detection rate was 69 %, decision count was 0.985, and lower limit of detection was 4 cells (0.004 %). We evaluated the CV% value of the number of …

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Discovery of a Hidden Trypanosoma cruzi Spermidine Synthase Binding Site and Inhibitors through In Silico, In Vitro , and X-ray Crystallography

Ryunosuke Yoshino, Nobuaki Yasuo, Yohsuke Hagiwara, Takashi Ishida et autres

High Resolution Image Download MS PowerPoint Slide In drug discovery research, the selection of promising binding sites and understanding the binding mode of compounds are crucial fundamental studies. The current understanding of the proteins-ligand binding model extends beyond the simple lock and …

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Discovery of a Hidden Trypanosoma cruzi Spermidine Synthase Binding Site and Inhibitors through In Silico, In Vitro, and X-ray Crystallography

龍ノ介 吉野, Ryunosuke Yoshino, 信明 安尾, Nobuaki Yasuo et autres

Screening Station, a novel laboratory automation system for physiologically relevant cell-based assays

Ichiji Namatame, Kana Ishii, Takashi Shin, Daisuke Shimojo et autres

Due to their physiological relevance, cell-based assays using human-induced pluripotent stem cell (iPSC)-derived cells are a promising in vitro pharmacological evaluation system for drug candidates. However, cell-based assays involve complex processes such as long-term culture, real-time and continuous observation of living cells, …

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Discovering a hidden binding site of spermidine synthase inhibitors for Chagas disease by combining molecular simulations and X-ray crystallography

Ryunosuke Yoshino, Nobuaki Yasuo, Yohsuke Hagiwara, Takashi Ishida et autres

Background Chagas disease is caused by the parasite Trypanosoma cruzi and is one of the neglected tropical diseases. Although two types of drugs are currently available, new drugs are still required because they have serious side effects. To develop a therapeutic agent …

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Discovering a hidden binding site of spermidine synthase inhibitors for Chagas disease by combining molecular simulations and X-ray crystallography

Ryunosuke Yoshino, Nobuaki Yasuo, Yohsuke Hagiwara, Takashi Ishida et autres

Background Chagas disease is caused by the parasite Trypanosoma cruzi and is one of the neglected tropical diseases. Although two types of drugs are currently available, new drugs are still required because they have serious side effects. To develop a therapeutic agent …

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A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth

Tomohiro Yoshida, Shingo Yamasaki, Osamu Kaneko, Naofumi Taoka et autres

Metabolic programs are rewired in cancer cells to support survival and tumor growth. Among these, recent studies have demonstrated that glutamate-oxaloacetate transaminase 1 (GOT1) plays key roles in maintaining redox homeostasis and proliferation of pancreatic ductal adenocarcinomas (PDA). This suggests that small …

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Correction to NMR Biochemical Assay for Oxidosqualene Cyclase: Evaluation of Inhibitor Activities on Trypanosoma cruzi and Human Enzymes

Osamu Tani, Yukie Akutsu, Shinji Ito, Takayuki Suzuki et autres

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to NMR Biochemical Assay for Oxidosqualene Cyclase: Evaluation of Inhibitor Activities on Trypanosoma cruzi and Human EnzymesOsamu TaniOsamu TaniMore by Osamu Tani, Yukie AkutsuYukie AkutsuMore by Yukie Akutsu, Shinji ItoShinji ItoMore by Shinji …

NMR Biochemical Assay for Oxidosqualene Cyclase: Evaluation of Inhibitor Activities on Trypanosoma cruzi and Human Enzymes

Osamu Tani, Yukie Akutsu, Shinji Ito, Takayuki Suzuki et autres

Oxidosqualene cyclase (OSC), a membrane-associated protein, is a key enzyme of sterol biosynthesis. Here we report a novel assay for OSC, involving reaction in aqueous solution, NMR quantification in organic solvent, and factor analysis of spectra. We evaluated one known and three …

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Crystal structures of FMN ‐bound and FMN ‐free forms of dihydroorotate dehydrogenase from Trypanosoma brucei

Tomomi Kubota, Osamu Tani, Tomohiko Yamaguchi, Ichiji Namatame et autres

Dihydroorotate dehydrogenase (DHODH) is a flavin‐binding enzyme essential for pyrimidine biosynthesis, which converts dihydroorotate to orotate. Three‐dimensional structures of cytosolic DHODH of parasitic protozoa are of interest in drug discovery for neglected tropical diseases, especially because these enzymes possess significantly different structural …

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