Hélène Royo

The histone H3 lysine 36 demethylase KDM2A/FBXL11 controls Polycomb-mediated gene repression and germ cell development in male mice

Michael Bocker, Grigorios Fanourgakis, Kristie Wetzel, Pavel A. Komarov et autres

KDM2A/FBXL11 is a Jumonji-domain containing lysine demethylase catalyzing the removal of mono- and di-methyl modifications of histone H3 lysine 36 (H3K36me1/2). While Kdm2a is required for mouse embryogenesis, its role in adult physiology has been largely unexplored. Using conditional deletion approaches, we …

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SUMOylated PRC1 controls histone H3.3 deposition and genome integrity of embryonic heterochromatin

Zichuan Liu, Mathieu Tardat, Mark E. Gill, Hélène Royo et autres

Chromatin integrity is essential for cellular homeostasis. Polycomb group proteins modulate chromatin states and transcriptionally repress developmental genes to maintain cell identity. They also repress repetitive sequences such as major satellites and constitute an alternative state of pericentromeric constitutive heterochromatin at paternal …

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Kmt2b conveys monovalent and bivalent H3K4me3 in mouse spermatogonial stem cells at germline and embryonic promoters

Shin-ichi Tomizawa, Y. Kobayashi, Takayuki Shirakawa, Kumiko Watanabe et autres

The mammalian male germline is sustained by a pool of spermatogonial stem cells (SSCs) that can transmit both genetic and epigenetic information to offspring. However, the mechanisms underlying epigenetic transmission remain unclear. The histone methyltransferase Kmt2b is highly expressed in SSCs and …

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Galaxy2Shiny2Galaxy

Hans-Rudolf Hotz, Hélène Royo, Michael B. Stadler

For our NGS analysis, we have combined Galaxy with Shiny. Each time a data set (e. g.: a count table from an RNA-Seq experiment) is created in Galaxy, a new app is set up on a local Shiny server. The Galaxy user …

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A Novel Inducible Mouse Model of MLL‐ENL‐driven Mixed‐lineage Acute Leukemia

Vaia Stavropoulou, Marwa Almosailleakh, Hélène Royo, Jean‐François Spetz et autres

Supplemental Digital Content is available in the text

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Non-Canonical and Sexually Dimorphic X Dosage Compensation States in the Mouse and Human Germline

Mahesh Sangrithi, Hélène Royo, Shantha K. Mahadevaiah, Obah A. Ojarikre et autres

Somatic X dosage compensation requires two mechanisms: X inactivation balances X gene output between males (XY) and females (XX), while X upregulation, hypothesized by Ohno and documented in vivo, balances X gene with autosomal gene output. Whether X dosage compensation occurs in …

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MLL-AF9 Expression in Hematopoietic Stem Cells Drives a Highly Invasive AML Expressing EMT-Related Genes Linked to Poor Outcome

Vaia Stavropoulou, Susanne Kaspar, Laurent Brault, Mathijs A. Sanders et autres

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Alternative Computational Analysis Shows No Evidence for Nucleosome Enrichment at Repetitive Sequences in Mammalian Spermatozoa

Hélène Royo, Michael Stadler, Antoine H.F.M. Peters

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Silencing of X-Linked MicroRNAs by Meiotic Sex Chromosome Inactivation

Hélène Royo, Hervé Seitz, Elias Elinati, Antoine H.F.M. Peters et autres

During the pachytene stage of meiosis in male mammals, the X and Y chromosomes are transcriptionally silenced by Meiotic Sex Chromosome Inactivation (MSCI). MSCI is conserved in therian mammals and is essential for normal male fertility. Transcriptomics approaches have demonstrated that in …

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ATR acts stage specifically to regulate multiple aspects of mammalian meiotic silencing

Hélène Royo, Haydn M. Prosser, Yaroslava Ruzankina, Shantha K. Mahadevaiah et autres

In mammals, homologs that fail to synapse during meiosis are transcriptionally inactivated. This process, meiotic silencing, drives inactivation of the heterologous XY bivalent in male germ cells (meiotic sex chromosome inactivation [MSCI]) and is thought to act as a meiotic surveillance mechanism. …

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Rsx is a metatherian RNA with Xist-like properties in X-chromosome inactivation

Jennifer Grant, Shantha K. Mahadevaiah, Pavel P. Khil, Mahesh Sangrithi et autres

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The RNase III Enzyme DROSHA Is Essential for MicroRNA Production and Spermatogenesis

Qiuxia Wu, Rui Song, Nicole Ortogero, Huili Zheng et autres

DROSHA is a nuclear RNase III enzyme responsible for cleaving primary microRNAs (miRNAs) into precursor miRNAs and thus is essential for the biogenesis of canonical miRNAs. DICER is a cytoplasmic RNase III enzyme that not only cleaves precursor miRNAs to produce mature …

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