Antoine H.F.M. Peters

Single molecule footprinting measures low nucleosome occupancy in mature spermatozoa of mice and men

Laura Gaspa-Toneu, H. Z. Shi, Evgeniy A. Ozonov, Mark E. Gill et autres

Abstract Nucleosomes are fundamental units of DNA packaging and gene regulation in eukaryotes. In mammalian sperm, most nucleosomes are replaced by protamines causing extreme chromatin compaction. Various epigenomic studies reported conflicting results on the distribution of residual nucleosomes in mammalian sperm 1–9 …

ch (code pays fourni par la source)

The eutherian-specific histone H3.4 promotes germ cell development and reproductive fitness

Pavel A. Komarov, Philipp C. Bammer, Ching-Yeu Liang, Hans-Rudolf Hotz et autres

Abstract Many genes encoding chromatin proteins are subject to evolutionary selection driving reproductive fitness. In mice and men, the histone H3.4 variant is essential to spermatogenesis. Here we define the evolutionary origin and molecular-physiological roles of sequence variation in H3f4 for male …

ch (code pays fourni par la source)

Raw uncropped images of blots

Antoine H.F.M. Peters

Many genes encoding chromatin proteins are subject to evolutionary selection driving reproductive fitness. In mice and men, the histone H3.4 variant (formerly known as H3t) is essential to spermatogenesis. Here we define the evolutionary origin and molecular-physiological roles of sequence variation in …

Raw uncropped images of blots

Antoine H.F.M. Peters

Many genes encoding chromatin proteins are subject to evolutionary selection driving reproductive fitness. In mice and men, the histone H3.4 variant (formerly known as H3t) is essential to spermatogenesis. Here we define the evolutionary origin and molecular-physiological roles of sequence variation in …

Raw uncropped images of blots

Antoine H.F.M. Peters

Many genes encoding chromatin proteins are subject to evolutionary selection driving reproductive fitness. In mice and men, the histone H3.4 variant (formerly known as H3t) is essential to spermatogenesis. Here we define the evolutionary origin and molecular-physiological roles of sequence variation in …

The eutherian-specific histone H3.4 promotes germ cell development and reproductive fitness

Antoine H.F.M. Peters

ch (code pays fourni par la source)

Preventing CpG hypermethylation in oocytes safeguards mouse development

Yumiko Kawamura, Evgeniy A. Ozonov, Panagiotis Papasaikas, Takashi Kondo et autres

Except for regulatory CpG-island sequences, genomes of most mammalian cells are widely DNA-methylated. In oocytes, though, DNA methylation (DNAme) is largely confined to transcribed regions. The mechanisms restricting de novo DNAme in oocytes and their relevance thereof for zygotic genome activation and …

ch, jp (code pays fourni par la source)

Chromatin Structure, Composition, and Function During Spermatogenesis

Antoine H.F.M. Peters, Mark E. Gill

ch (code pays fourni par la source)

Preventing CpG hypermethylation in oocytes safeguards mouse development

Antoine H.F.M. Peters

ch (code pays fourni par la source)

The histone H3 lysine 36 demethylase KDM2A/FBXL11 controls Polycomb-mediated gene repression and germ cell development in male mice

Michael Bocker, Grigorios Fanourgakis, Kristie Wetzel, Pavel A. Komarov et autres

KDM2A/FBXL11 is a Jumonji-domain containing lysine demethylase catalyzing the removal of mono- and di-methyl modifications of histone H3 lysine 36 (H3K36me1/2). While Kdm2a is required for mouse embryogenesis, its role in adult physiology has been largely unexplored. Using conditional deletion approaches, we …

us, ch, jp, nl (code pays fourni par la source)

The histone H3 lysine 36 demethylase KDM2A/FBXL11 controls Polycomb-mediated gene repression and germ cell development in male mice

Antoine H.F.M. Peters

ch (code pays fourni par la source)

A statistical model for quantitative analysis of single-molecule footprinting data

Evgeniy A. Ozonov, Laura Gaspa-Toneu, Antoine H.F.M. Peters

ABSTRACT The binding of sequence-specific TFs (TF) to genomic DNA is fundamental to gene regulation. Emerging single-molecule footprinting (SMF) technologies such as the NOMe-seq and Fiber-seq assays offer unique opportunities for acquiring quantitative information about binding states of TFs and nucleosomes at …

ch (code pays fourni par la source)