Deborah Byrne

Nitroxide spin, non-canonical amino acids and SDSL-EPR spectroscopy: a winning combination for protein dynamics studies

Maxime Bizet, Áron Balázsi, Colette Maignet-Magard, Pierre Dorlet et autres

fr, hu (code pays fourni par la source)

Impact of CP12 deletion on inorganic carbon acquisition and Rubisco partitioning in Chlamydomonas reinhardtii

Cassy Gérard, Régine Lebrun, Christophe Verthuy, Hugo Le Guenno et autres

The small chloroplastic protein CP12 has multiple functions, including the regulation of enzymes in the Calvin-Benson-Bassham cycle. Here, we investigated its role in the acclimation of Chlamydomonas reinhardtii to varying CO2 availability. We show that phosphoribulokinase can interact with CP12 in conditions …

fr, kr, gb (code pays fourni par la source)

Subtle Variations in a Client Protein Determine Bacterial Hsp90 Dependence

Marie Corteggiani, Amine Ali-Chaouche, Miha Bahun, Flora Ambre Honoré et autres

Chaperones ensure protein homeostasis and are conserved across species. The ATP-dependent chaperone Hsp90 is present from bacteria to eukaryotes, where it stabilizes and activates a wide range of substrate proteins called clients. However, what determines whether a protein depends on Hsp90 remains …

fr, si, ch (code pays fourni par la source)

Subtle variations in a client protein determine bacterial Hsp90 dependence

Marie Corteggiani, Amine Ali Chaouche, Miha Bahun, Flora Ambre Honoré et autres

Abstract Chaperones ensure protein homeostasis and are conserved across species. The ATP-dependent chaperone Hsp90 is present from bacteria to eukaryotes, where it stabilizes and activates a wide range of substrate proteins called clients. However, what determines whether a protein depends on Hsp90 …

fr, ca, si, ch (code pays fourni par la source)

Expanding the Diversity of Nitroxide‐Based Paramagnetic Probes Conjugated to Non‐Canonical Amino Acids for Sdsl‐Epr Applications

Maxime Bizet, Áron Balázsi, Frédéric Biaso, Deborah Byrne et autres

Abstract Understanding protein structure requires studying its dynamics, which is critical to elucidating its functional role. Biophysical techniques have revolutionized this field over time, providing remarkable insights into structure‐function relationships. Among these, Site‐Directed Spin Labelling (SDSL) combined with Electron Paramagnetic Resonance (EPR) …

fr, hu, us (code pays fourni par la source)

Expanding the diversity of nitroxide-based paramagnetic probes conjugated to non-canonical amino acids for SDSL-EPR applications

Maxime Bizet, Áron Balázsi, Frédéric Biaso, Deborah Byrne et autres

Abstract Understanding protein structure requires studying its dynamics, which is critical to elucidating its functional role. Over time, biophysical techniques have revolutionized this field, offering remarkable insights into the structure-function relationship. Among these, Site-Directed Spin Labelling (SDSL) combined with Electron Paramagnetic Resonance …

fr, hu, us (code pays fourni par la source)

Structural insights into the semiquinone form of human Cytochrome P450 reductase by DEER distance measurements between a native flavin and a spin labelled non‐canonical amino acid

Maxime Bizet, Deborah Byrne, Frédéric Biaso, Guillaume Gerbaud et autres

Abstract The flavoprotein Cytochrome P450 reductase (CPR) is the unique electron pathway from NADPH to Cytochrome P450 (CYPs). The conformational dynamics of human CPR in solution, which involves transitions from a “locked/closed” to an “unlocked/open” state, is crucial for electron transfer. To …

fr, hu (code pays fourni par la source)

The YmgB-SpoT interaction triggers the stringent response in Escherichia coli

Paul Guiraud, Elsa Germain, Deborah Byrne, Etienne Maisonneuve

Virtually all bacterial species synthesize (p)ppGpp (guanosine penta- or tetraphosphate), a pleiotropic regulator of the so-called stringent response, which controls many aspects of cellular physiology and metabolism. In Escherichia coli, (p)ppGpp levels are controlled by two homologous enzymes: the (p)ppGpp synthetase RelA …

fr (code pays fourni par la source)

Bacterial adaptation to cold: Conservation of a short J‐domain co‐chaperone and its protein partners in environmental proteobacteria

L. Weber, Atar Gilat, Nathanael Jean Maillot, Deborah Byrne et autres

Abstract Bacterial genomes are a huge reservoir of genes encoding J‐domain protein co‐chaperones that recruit the molecular chaperone DnaK to assist protein substrates involved in survival, adaptation, or fitness. The atc operon of the aquatic mesophilic bacterium Shewanella oneidensis encodes the proteins …

fr, il (code pays fourni par la source)

A qualitative analysis of women’s postnatal experiences of breastfeeding supports during the perinatal period in Ireland

Niamh Lawlor, Lucia Prihodová, Deborah Byrne, Megan Etherton et autres

Ireland has among the lowest rates of breastfeeding worldwide. Despite policies to support breastfeeding, breastfeeding initiation and exclusivity remain low in Ireland. Greater knowledge about support received in the maternity unit may-in part-shed light on why this is so. Our aim was …

ie (code pays fourni par la source)

Using Simulation to Develop Clinical Judgment in Undergraduate Nursing Students

Deborah Byrne, S. M. Blumenfeld, Maureen Szulewski, Rita Ann Laske

The National Council of State Boards of Nursing plans to update the National Council Licensure Exam for nursing graduates to better measure clinical judgment. It is important that schools of nursing provide opportunities for nursing students to practice and develop clinical judgment …

us (code pays fourni par la source)

Evidence for [2Fe-2S]2+ and Linear [3Fe-4S]1+ Clusters in a Unique Family of Glycine/Cysteine-Rich Fe-S Proteins from Megavirinae Giant Viruses

Alejandro Villalta, Audrey Lartigue, Martin Clémancey, Deborah Byrne et autres

We have discovered a protein with an amino acid composition exceptionally rich in glycine and cysteine residues in the giant virus mimivirus. This small 6 kDa protein is among the most abundant proteins in the icosahedral 0.75 μm viral particles; it has …

fr (code pays fourni par la source)