Nicolas von Ahsen

Correspondence

Nicolas von Ahsen

Early Pregnancy Loss in the 1st Trimester

Matthias David, Nicolas von Ahsen, İbrahim Alkatout, Franz Bahlmann et autres

Abstract Purpose This guideline aims to improve and standardize the diagnostic and therapeutic approaches for different types of miscarriages, pregnancies of unclear localization, and ectopic pregnancies in the 1st trimester. Methods In accordance with the requirements for an S2k-guideline, this guideline was …

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Comparison and Harmonization of Different Semi-Automated and Automated qRT-PCR Assays in the Assessment of SARS-CoV-2

Sascha Dierks, Karin Thiele, Wolfgang Bohne, Raimond Lugert et autres

In SARS-CoV-2 diagnostics, cycle threshold (Ct) values from qRT-PCRs semi-quantitatively estimate a patient’s viral load. However, relevant analytical differences between qRT-PCR assays are often neglected. This study was designed (i) to identify such differences between five commonly used assays and (ii) to …

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Is There a “Blind Spot” in Point-of-Care Testing for Residual Heparin After Cardiopulmonary Bypass? A Prospective, Observational Cohort Study

Saskia Wand, Daniel Heise, Nadine Hillmann, Christian Bireta et autres

Identifying the cause of a bleeding complication after cardiac surgery can be crucial. This study sought to clarify whether the application of unprocessed autologous pump blood influences anti-factor Xa activity after cardiac surgery and evaluated 2 point-of-care methods regarding their ability to …

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Macro-CK type 2 in metastatic prostate cancer

Abass Eidizadeh, Nicolas von Ahsen, Steffen Friedewald, Lutz Binder

The isoenzyme creatine kinase muscle/brain (CK-MB) still plays an important role for the differential diagnosis of CK elevations and the clarification of their origin from heart or skeletal muscle. Therefore, it is necessary to know the diagnostic pitfalls in interpreting CK-MB results. …

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Differences in procalcitonin measurements between three BRAHMS-partnered immunoassays (Liaison, Elecsys and Architect)

Abass Eidizadeh, Abdul R. Asif, Nicolas von Ahsen, Lutz Binder et autres

Article Differences in procalcitonin measurements between three BRAHMS-partnered immunoassays (Liaison, Elecsys and Architect) was published on September 1, 2019 in the journal Clinical Chemistry and Laboratory Medicine (CCLM) (volume 57, issue 9).

Validation of a high-performance liquid chromatography method for thiopurine S-methyltransferase activity in whole blood using 6-mercaptopurine as substrate

Hannah Rieger, Patrik Schmidt, Elke Schaeffeler, Manabu Abe et autres

BACKGROUND: Variation in metabolism, toxicity and therapeutic efficacy of thiopurine drugs is largely influenced by genetic polymorphisms in the thiopurine S-methyltransferase (TPMT) gene. Determination of TPMT activity is routinely performed in patients to adjust drug therapy. METHODS: We further optimized a previously …

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Single-Nucleotide Polymorphism of CYP3A5 Impacts the Exposure to Tacrolimus in Pediatric Renal Transplant Recipients: A Pharmacogenetic Substudy of the TWIST Trial

Heiko Billing, Britta Höcker, Alexander Fichtner, Rita Van Damme‐Lombaerts et autres

BACKGROUND: The pharmacokinetics of tacrolimus (TAC) and mycophenolic acid (MPA) are highly variable. An impact of single-nucleotide polymorphisms (SNPs) of the genes coding for enzymes and transporters involved in the pharmacokinetics of TAC and/or MPA is intuitively conceivable. Accordingly, we sought to …

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Analytical evaluation of a real-time PCR-based DNA demethylation assay to assess the frequency of naturally occurring regulatory T cells in peripheral blood

Maria Metzker, Maria Shipkova, Nicolas von Ahsen, Rainer Andag et autres

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Analytical aspects of TDM of psychoactive drugs

Nicolas von Ahsen

Therapeutic drug monitoring (TDM) is an established means to improve safety and efficacy of treatment with psychoactive drugs. The plasma level is used as a surrogate marker for availability of the drug at its targets with the numerical result taken for granted. …

Longitudinal analyses of anti-JCV antibody index for risk assessment of progressive multifocal leukoencephalopathy

Anke Salmen, Nicolas von Ahsen, A.K. Trampe, Robert Hoepner et autres

Risk assessment for natalizumab-associated progressive multifocal leukoencephalopathy (Nat-PML) comprises the anti-JC virus (JCV) antibody index (AI). The anti-JCV AI was longitudinally determined in a natalizumab-treated MS cohort (Nat-MS, n = 468) and samples of Nat-PML patients ( n = 15). In Nat-MS, …

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Lack of correlation between Treg quantification assays in inflammatory bowel disease patients

Gunnar Brandhorst, Darinka Todorova Petrova, Sebastian Weigand, Christoph Eberle et autres

AIM: To compare the number of regulatory T-cells (Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR (qPCR) method in patients suffering from inflammatory bowel disease (IBD). METHODS: Tregs percentages obtained by both flow cytometry and qPCR methods …