Darinka Todorova Petrova

Mycophenolic acid reverses TGF beta‐induced cell motility, collagen matrix contraction and cell morphology in vitro

Darinka Todorova Petrova, Gunnar Brandhorst, Christian A. Koch, Frank Christian Schultze et autres

The aim of this study was to elucidate functional and molecular effects of mycophenolic acid (MPA) on non-lymphatic, kidney epithelial cells treated with transforming growth factor (TGF). MPA effects were studied using HK2 cells incubated with EGF and TGF. The reversibility of …

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Lack of correlation between Treg quantification assays in inflammatory bowel disease patients

Gunnar Brandhorst, Darinka Todorova Petrova, Sebastian Weigand, Christoph S. Eberle et autres

AIM: To compare the number of regulatory T-cells (Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR (qPCR) method in patients suffering from inflammatory bowel disease (IBD). METHODS: Tregs percentages obtained by both flow cytometry and qPCR methods …

Effects of mycophenolate mofetil on kidney function and phosphorylation status of renal proteins in Alport COL4A3-deficient mice

Darinka Todorova Petrova, Frank Christian Schultze, Gunnar Brandhorst, Klaus-Dieter Luchs et autres

BACKGROUND: We investigated the effects of mycophenolate mofetil (MMF) on kidney function and on protein phosphorylation in a mouse model for the human Alport syndrome. METHODS: COL4A3-deficient (COL4A3-/-) mice were randomly allocated to receive a placebo (PLC COL4A3-/-) or MMF treatment (MMF …

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Comparability and Imprecision of 8 Frequently Used Commercially Available Immunoassays for Therapeutic Drug Monitoring

Maria Shipkova, Darinka Todorova Petrova, Alexander E. Rosler, Matthias Orth et autres

BACKGROUND: The development of new immunoassays for therapeutic drug monitoring and the adaptation of current assays on new analytical platforms has led to a plethora of different tests. This variability may influence comparability between the methods and affect interpretation of test results …

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FoxP3 demethylation is increased in human colorectal cancer and rat cholangiocarcinoma tissue

Frank Christian Schultze, Reiner Andag, Salamah Mohammad Alwahsh, Draga Ivanova Toncheva et autres

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Can the Roche hemolysis index be used for automated determination of cell-free hemoglobin? A comparison to photometric assays

Darinka Todorova Petrova, Gabriela Ariadna Cocisiu, Christoph S. Eberle, Karl-Heinz Rhode et autres

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Differential Kidney Proteome Profiling in a Murine Model of Renal Fibrosis under Treatment with Mycophenolate Mofetil

Darinka Todorova Petrova, Franziska Brehmer, Frank Christian Schultze, Abdul Rahman Asif et autres

AIM: The aim of this study was to investigate the effect of mycophenolate mofetil (MMF) using differential kidney proteome profiling of COL4A3-deficient mice as a model of progressive renal disease. METHODS: Histological evaluation of kidney sections was performed. Total protein lysate from …

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Supplementary Material for: Differential Kidney Proteome Profiling in a Murine Model of Renal Fibrosis under Treatment with Mycophenolate Mofetil

Darinka Todorova Petrova, Franziska Brehmer, Frank Christian Schultze, Abdul Rahman Asif et autres

Aim: The aim of this study was to investigate the effect of mycophenolate mofetil (MMF) using differential kidney proteome profiling of COL4A3-deficient mice as a model of progressive renal disease. Methods: Histological evaluation of kidney sections was performed. Total protein lysate from …

Comparability and imprecision of eight frequently used commercially available immunoassays for therapeutic drug monitoring

Maria Shipkova, Darinka Todorova Petrova, Alexander E. Rosler, M. Orth et autres

Mycophenolic Acid Displays IMPDH-Dependent and IMPDH-Independent Effects on Renal Fibroblast Proliferation and Function

Darinka Todorova Petrova, Gunnar Brandhorst, Franziska Brehmer, Oliver Groß et autres

The aim of this study was to elucidate the role of mycophenolic acid (MPA) in cellular pathways of renal fibrosis. Different assays were applied in a renal fibroblast model using COS-7 cells: assays for cell proliferation, scratch wound closure and collagen matrix …

Differential proteome and phosphoproteome signatures in human T‐lymphoblast cells induced by sirolimus

Frank Christian Schultze, Darinka Todorova Petrova, Michael Oellerich, Victor W. Armstrong et autres

OBJECTIVES: The present study was designed to investigate early proteome and phosphoproteome changes during inhibition of lymphocyte proliferation induced by sirolimus (SRL). MATERIALS AND METHODS: Proliferation assays were conducted using human CCRF-CEM T lymphoblasts under different SRL concentrations. Total protein lysates after …

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Mycophenolic Acid Predose Concentrations and Renal Function in a Mouse Model for Progressive Renal Fibrosis

Gunnar Brandhorst, Franziska Brehmer, Darinka Todorova Petrova, Oliver Groß et autres

Within the scope of this study, the potential antifibrotic effect of mycophenolate mofetil (MMF) on COL4A3-deficient mice as an animal model for progressive renal fibrosis was investigated regarding kidney function and survival. Thirty-five animals were randomly assigned to one of five groups …

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