Leticia Ramírez‐Silva

The Functional State of Thermoplasma acidophilum Pyruvate Kinase Relies on an Extra Carboxyl-Terminal Sequence

Leticia Ramírez‐Silva, Héctor Riveros‐Rosas, Gloria Hernández‐Alcántara, José J. García‐Trejo et autres

Phylogenetic studies of the pyruvate kinase family reveal two clusters: the K+-dependent and -independent enzymes. Thermoplasma acidophilum pyruvate kinase belongs to the latter but possesses the conserved signature of those K+-dependent. Recently, we found two distinct ways for these groups to catalyze. …

mx (code pays fourni par la source)

A New Real-Time Simple Method to Measure the Endogenous Nitrate Reductase Activity (Nar) in Paracoccus denitrificans and Other Denitrifying Bacteria

José J. García‐Trejo, Sharon Rojas-Alcantar, Monserrat Alonso-Vargas, Raquel Ortega et autres

The transmembrane nitrate reductase (Nar) is the first enzyme in the dissimilatory alternate anaerobic nitrate respiratory chain in denitrifying bacteria. To date, there has been no real-time method to determine its specific activity embedded in its native membrane; here, we describe such …

mx (code pays fourni par la source)

El papel de la isoforma M2 de la piruvato cinasa en el metabolismo del cáncer

Antonio Ponce-Tecla, Leticia Ramírez‐Silva, Gloria Hernández‐Alcántara

En la oncogénesis los distintos tipos de cáncer presentan alteraciones en el funcionamiento de sus diversas proteínas reguladoras, así como las proteínas que normalmente no están relacionadas con el control del ciclo celular. Es el caso de la piruvato cinasa en su …

mx (code pays fourni par la source)

Inhibitory to non-inhibitory evolution of the ζ subunit of the F1FO-ATPase of Paracoccus denitrificans and α-proteobacteria as related to mitochondrial endosymbiosis

Francisco Mendoza-Hoffmann, Lingyun Yang, Damiano Buratto, Jorge Brito-Sánchez et autres

Introduction: The ζ subunit is a potent inhibitor of the F 1 F O -ATPase of Paracoccus denitrificans (PdF 1 F O -ATPase) and related α -proteobacteria different from the other two canonical inhibitors of bacterial ( ε ) and mitochondrial (IF …

cn, mx (code pays fourni par la source)

The K+-Dependent and -Independent Pyruvate Kinases Acquire the Active Conformation by Different Mechanisms

Leticia Ramírez‐Silva, Gloria Hernández‐Alcántara, Carlos Guerrero-Mendiola, Martín González‐Andrade et autres

Eukarya pyruvate kinases possess glutamate at position 117 (numbering of rabbit muscle enzyme), whereas bacteria have either glutamate or lysine. Those with E117 are K+-dependent, whereas those with K117 are K+-independent. In a phylogenetic tree, 80% of the sequences with E117 are …

mx (code pays fourni par la source)

Exploring the differences between the three pyruvate kinase isozymes from Vibrio cholerae in a heterologous expression system

Zoe Alba-Martínez, Leticia Ramírez‐Silva, Gloria Hernández‐Alcántara

OBJECTIVE: The genome of Vibrio cholerae has three paralog genes encoding for distinct pyruvate kinases. We were interested in elucidating whether they were expressed, and contributed to the pyruvate kinase activity of V. cholerae. VcIPK and VcIIPK were transformed and expressed in …

mx (code pays fourni par la source)

The contribution of two isozymes to the pyruvate kinase activity of Vibrio cholerae: One K+-dependent constitutively active and another K+-independent with essential allosteric activation

Carlos Guerrero-Mendiola, José J. García‐Trejo, Rusely Encalada, Emma Saavedra et autres

In a previous phylogenetic study of the family of pyruvate kinase EC (2.7.1.40), a cluster with Glu117 and another with Lys117 were found (numbered according to the rabbit muscle enzyme). The sequences with Glu117 have been found to be K+-dependent, whereas those …

mx (code pays fourni par la source)

Proteins in Water–Cosolvent Binary Systems: Function and Structure

Leticia Ramírez‐Silva, Jesús Oria‐Hernández, Salvador Uribe‐Carvajal

Correction: New Insights on the Mechanism of the K+-Independent Activity of Crenarchaeota Pyruvate Kinases

Gustavo De la Vega-Ruíz, Lenin Domínguez‐Ramírez, Héctor Riveros‐Rosas, Carlos Guerrero-Mendiola et autres

There are errors in the Funding section. The complete, correct Funding information is as follows: This work was supported by Direccion General de Apoyo al Personal Academico- Universidad Nacional Autonoma de Mexico, Grants IN215912 (to L.R.-S.), IN216513 (to H.R.-R.), IA202714-2 (to G.H.-A.), …

New Insights on the Mechanism of the K+-Independent Activity of Crenarchaeota Pyruvate Kinases

Gustavo De la Vega-Ruíz, Lenin Domínguez‐Ramírez, Héctor Riveros‐Rosas, Carlos Guerrero-Mendiola et autres

Eukarya pyruvate kinases have glutamate at position 117 (numbered according to the rabbit muscle enzyme), whereas in Bacteria have either glutamate or lysine and in Archaea have other residues. Glutamate at this position makes pyruvate kinases K+-dependent, whereas lysine confers K+-independence because …

mx (code pays fourni par la source)

The Importance of Polarity in the Evolution of the K+ Binding Site of Pyruvate Kinase

Leticia Ramírez‐Silva, Carlos Guerrero-Mendiola, Nallely Cabrera

In a previous phylogenetic study of the family of pyruvate kinase, we found one cluster with Glu117 and another with Lys117. Those sequences with Glu117 have Thr113 and are K+-dependent, whereas those with Lys117 have Leu113 and are K+-independent. The carbonyl oxygen …

mx (code pays fourni par la source)

The ζ subunit of the F 1 F O ‐ATP synthase of α‐proteobacteria controls rotation of the nanomotor with a different structure

Mariel Zarco‐Zavala, Edgar Morales‐Ríos, Guillermo Mendoza‐Hernández, Leticia Ramírez‐Silva et autres

ABSTRACT The ζ subunit is a novel natural inhibitor of the α‐proteobacterial F 1 F O ‐ATPase described originally in Paracoccus denitrificans . To characterize the mechanism by which this subunit inhibits the F 1 F O nanomotor, the ζ subunit of …

mx, gb (code pays fourni par la source)