Giuseppe Longobardi

Double-coated PLGA nanoparticles with hierarchical surface architecture for CD44-targeted siRNA delivery

Giuseppe Longobardi, Pini Shekhter, Claudia Conte, Ronit Satchi-Fainaro et autres

Abstract Efficient delivery of small interfering RNA (siRNA) remains a materials challenge because it requires nanocarriers that stabilize polyanionic cargo, support cellular interactions, and enable cytosolic delivery. Although poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) are used due to biocompatibility, biodegradability, and regulatory acceptance, …

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Abstract LB329: Overcoming the blood-brain barrier to potentiate GD2-CAR T therapy using P-selectin-targeted nanomedicine

Giuseppe Longobardi, Anna Miari, Yulia Liubomirski, Ester Buderovsky et autres

Abstract Pediatric brain tumors are the leading cause of cancer-related death in children, and high-grade malignancies such as diffuse intrinsic pontine glioma (DIPG) and medulloblastoma (MB) remain intractable. DIPG is inoperable due to its brainstem location and diffuse infiltration, while MB therapy …

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Abstract LB232: BRCA1 deficiency programs a microglia-supported ferroptosis-resistant niche in breast cancer brain metastasis

Rami Khoury, Kathrin Laue, Giuseppe Longobardi, Shahar Greenberg et autres

Abstract Several mutations have been identified in triple-negative breast cancer (TNBC), including deleterious mutations in BRCA1. Despite advances in understanding and managing BRCA1-associated TNBC, the brain metastatic stage of this disease remains largely understudied. This study sought to identify genotype-specific tumor-microglia interactions …

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Harnessing Next‐Generation 3D Cancer Models to Elucidate Tumor‐Microbiome Crosstalk (Adv. Healthcare Mater. 1/2026)

Marina Green Buzhor, Giuseppe Longobardi, Or Kandli, Anne Krinsky et autres

3D Cancer Models This illustration presents a schematic construction site where tumor masses are built as avatars composed of cancer, immune, stromal cells, and blood vessels. Tools and machines represent advanced 3D technologies, while a truck delivers bacteria to model tumor-microbiome crosstalk, …

p53 inactivation drives breast cancer metastasis to the brain through SCD1 upregulation and increased fatty acid metabolism

Kathrin Laue, Sabina Pozzi, Johanna Zerbib, Rebecca Bertolio et autres

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Harnessing Next‐Generation 3D Cancer Models to Elucidate Tumor‐Microbiome Crosstalk

Marina Green Buzhor, Giuseppe Longobardi, Or Kandli, Anne Krinsky et autres

The tumor microenvironment (TME) is a complex and dynamic ecosystem increasingly recognized for its interplay with the microbiome. In colorectal, breast, lung, liver, and brain cancers, bacterial communities and their metabolites are shown to influence tumor progression, immune responses, and therapeutic outcomes. …

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Radiation-guided nanoparticles enhance the efficacy of PARP inhibitors in primary and metastatic BRCA1-deficient tumors via immunotherapy

Rami Khoury, Giuseppe Longobardi, Tania Barnatan, Dana Venkert et autres

Poly (ADP-ribose) polymerase inhibitors (PARPi) have revolutionized the treatment landscape for patients suffering from BRCA1- mutated breast and ovarian cancers. However, responses are not durable. We demonstrate that treatment with the PARPi, niraparib, increases programmed cell death protein-1 ligand (PD-L1) expression in …

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Light‐Activatable Hyaluronic Acid‐Derivatives Releasing Nitric Oxide and Their Delivery in the Skin

Giuseppe Longobardi, Cristina Parisi, Federica Sodano, Ovidio Catanzano et autres

Hyaluronic acid (HA), renowned for its hydrating properties, penetrates deeper into the skin at a very low molecular weight, promoting cellular repair and reducing inflammation. Nitric oxide (NO), a gaseous mediator with a very relevant role in many physiological and pathophysiological processes, …

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Polyester nanoparticles delivering chemotherapeutics: Learning from the past and looking to the future to enhance their clinical impact in tumor therapy

Giuseppe Longobardi, Thomas L. Moore, Claudia Conte, Francesca Ungaro et autres

Polymeric nanoparticles (NPs), specifically those comprised of biodegradable and biocompatible polyesters, have been heralded as a game-changing drug delivery platform. In fact, poly(α-hydroxy acids) such as polylactide (PLA), poly(lactide-co-glycolide) (PLGA), and poly(ε-caprolactone) (PCL) have been heavily researched in the past three decades …

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Enhancing siRNA cancer therapy: Multifaceted strategies with lipid and polymer-based carrier systems

Nazgol Karimi Dastgerdi, Nazanin Karimi Dastgerdi, Hulya Bayraktutan, Gabriella Costabile et autres

Cancers are increasing in prevalence and many challenges remain for their treatment, such as chemoresistance and toxicity. In this context, siRNA-based therapeutics have many potential advantages for cancer therapies as a result of their ability to reduce or prevent expression of specific …

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Development and Nanoparticle-Mediated Delivery of Novel MDM2/MDM4 Heterodimer Peptide Inhibitors to Enhance 5-Fluorouracil Nucleolar Stress in Colorectal Cancer Cells

Francesco Merlino, Annalisa Pecoraro, Giuseppe Longobardi, Greta Donati et autres

Colorectal cancer (CRC) often involves wild-type p53 inactivation by MDM2 and MDM4 overexpression, promoting tumor progression and resistance to 5-fluoruracil (5-FU). Disrupting the MDM2/4 heterodimer can proficiently reactivate p53, sensitizing cancer cells to 5-FU. Herein, we developed 16 peptides based on Pep3 …

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Supramolecular red-light-photosensitized nitric oxide release with fluorescence self-reporting within biocompatible nanocarriers

Francesca Laneri, Cristina Parisi, Mimimorena Seggio, Aurore Fraix et autres

The strict dependence of the biological effects of nitric oxide (NO) on its concentration and generation site requires this inorganic free radical to be delivered with precise spatiotemporal control. Light-activation by suitable NO photoprecursors represents an ideal approach. Developing strategies to activate …

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