Ana Marcela Giudici

Lipid modulation in a model K+ channel occurs through alterations at both, the inner and the outer channel gates

Carlos Coll-Díez, Ana Marcela Giudici, Javier Soriano Botella, José M. González‐Ros et autres

ABSTRACT This paper reports on a plausible mechanism of modulation by anionic phospholipids of KcsA, a model prokaryotic potassium channel. A first conclusion is that the association of anionic phospholipids with KcsA occurs at discrete regions located near both the extracellular and …

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Lipid modulation in a model K+ channel occurs through alterations at both, the inner and the outer channel gates

Carlos Coll-Díez, Ana Marcela Giudici, Javier Soriano Botella, José M. González‐Ros et autres

The intrinsically disordered protein NUPR1 binds to phospholipids

Matías Estarás, Bruno Rizzuti, Ana Marcela Giudici, José A. Poveda et autres

The nuclear protein 1 (NUPR1) is an intrinsically disordered protein (IDP) involved in stress-mediated cellular conditions, with an interactome including many other partner proteins, as well as nucleic acids. We wondered whether its great conformational flexibility and biological versatility could include interactions …

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The Citrullinating Enzyme PADI4 Binds to Lipids: Identification of New Target Interactions for Cancer Therapy

Salomé Araujo‐Abad, Borja García-Peñarrubia, Ana Marcela Giudici, José L. Neira et autres

Cancer cells need to implement and maintain molecular mechanisms relying on an energy trade-off between resistance and key functions to survive. Among them, changes in lipid metabolism are crucial. Phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidic acid (PA) are some of the lipids …

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The Diacylglycerol Acyltransferase 3 of Chlamydomonas reinhardtii Is a Disordered Protein Capable of Binding to Lipids Derived from Chloroplasts

Natalia Pavia, Alberto Potenza, Felipe Hornos, José A. Poveda et autres

Understanding triacylglycerol (TAG) metabolism is crucial for developing algae as a source of biodiesel. TAGs are the main reservoir of energy in most eukaryotes. The final, rate-limiting step in the formation of TAGs is catalyzed by 1,2-diacylglycerol acyltransferases (DGATs). In the green …

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pH-induced conformational changes in the selectivity filter of a potassium channel lead to alterations in its selectivity and permeation properties

Carlos Coll-Díez, Ana Marcela Giudici, Alberto Potenza, José M. González‐Ros et autres

The Selectivity Filter (SF) in tetrameric K+ channels, has a highly conserved sequence, TVGYG, at the extracellular entry to the channel pore region. There, the backbone carbonyl oxygens from the SF residues, create a stack of K+ binding sites where dehydrated K+ …

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Steady-state and time-resolved fluorescent methodologies to characterize the conformational landscape of the selectivity filter of K+ channels

M. Lourdes Renart, Ana Marcela Giudici, José M. González‐Ros, José A. Poveda

A variety of equilibrium and non-equilibrium methods have been used in a multidisciplinary approach to study the conformational landscape associated with the binding of different cations to the pore of potassium channels. These binding processes, and the conformational changes resulting therefrom, modulate …

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Anionic Phospholipids Shift the Conformational Equilibrium of the Selectivity Filter in the KcsA Channel to the Conductive Conformation: Predicted Consequences on Inactivation

M. Lourdes Renart, Ana Marcela Giudici, Carlos Coll-Díez, José M. González‐Ros et autres

Here, we report an allosteric effect of an anionic phospholipid on a model K+ channel, KcsA. The anionic lipid in mixed detergent–lipid micelles specifically induces a change in the conformational equilibrium of the channel selectivity filter (SF) only when the channel inner …

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Molecular Events behind the Selectivity and Inactivation Properties of Model NaK-Derived Ion Channels

Ana Marcela Giudici, M. Lourdes Renart, Ana Coutinho, Andrés Morales et autres

Y55W mutants of non-selective NaK and partly K+-selective NaK2K channels have been used to explore the conformational dynamics at the pore region of these channels as they interact with either Na+ or K+. A major conclusion is that these channels exhibit a …

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Biochemical and biophysical characterization of PADI4 supports its involvement in cancer

José L. Neira, Salomé Araujo‐Abad, A. Cámara-Artigas, Bruno Rizzuti et autres

PADI4 (protein-arginine deiminase, also known as protein l-arginine iminohydrolase) is one of the human isoforms of a family of Ca2+-dependent proteins catalyzing the conversion of arginine to citrulline. Although the consequences of this process, known as citrullination, are not fully understood, all …

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Insights into the Conformational Dynamics of Potassium Channels Using Homo-FRET Approaches

Ana Coutinho, Clara Díaz-García, Ana Marcela Giudici, M. Lourdes Renart

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Probing the Structural Dynamics of the Activation Gate of KcsA Using Homo-FRET Measurements

Clara Díaz-García, M. Lourdes Renart, José A. Poveda, Ana Marcela Giudici et autres

The allosteric coupling between activation and inactivation processes is a common feature observed in K+ channels. Particularly, in the prokaryotic KcsA channel the K+ conduction process is controlled by the inner gate, which is activated by acidic pH, and by the selectivity …

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