Burckhard Seelig

BPS2026 – Functional characterization of de novo, primordial-like proteins

Peter J. Winslow, Samuel Erickson, Burckhard Seelig

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EasyDIVER + : An Advanced Tool for Analyzing High Throughput Sequencing Data from In Vitro Evolution of Nucleic Acids or Amino Acids

Celia Blanco, Allison Tee, Matilda Newton, Kun‐Hwa Lee et autres

In vitro evolution is a powerful technique for identifying functional nucleic acids and peptides, but the analysis of the resulting high-throughput sequencing data poses significant challenges, particularly in peptide selections. Existing bioinformatics tools often lack the specificity needed for this task, leaving …

us, nz (code pays fourni par la source)

Did Early Functional Proteins Use Metal Ions to Bind ATP?

Alexander D. Soltau, Eli Blascyk, Peter Winslow, Burckhard Seelig

UROP Project conducted in the Seelig Lab under the direction of Dr. Burckhard Seelig and graduate student Peter J. Winslow.

Intellectual frameworks to understand complex biochemical systems at the origin of life

Burckhard Seelig, Irene A. Chen

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Comprehensive protease specificity profiling

Bo Zhu, Michael D. Lane, Joshua A. Baller, Pratik Jagtap et autres

Abstract Protease enzymes are of great importance in medicine, industry, and as research tools. Despite the crucial need for detailed knowledge of their proteolytic cleavage specificity, many proteases are poorly characterized. We present a method for fully characterizing the cleavage specificity of …

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Faculty Opinions recommendation of An enzyme cascade enables production of therapeutic oligonucleotides in a single operation.

Burckhard Seelig

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Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets

Bo Zhu, Zhe Du, Yancen Dai, Tetsuya Kitaguchi et autres

In vitro compartmentalization (IVC) is a technique for generating water-in-oil microdroplets to establish the genotype (DNA information)-phenotype (biomolecule function) linkage required by many biological applications. Recently, fluorinated oils have become more widely used for making microdroplets due to their better biocompatibility. However, …

jp, us, cn (code pays fourni par la source)

Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B

Kun‐Hwa Lee, Mark D. Distefano, Burckhard Seelig

Pyridoxal 5′-phosphate (PLP) is a ubiquitous and versatile cofactor utilized by numerous enzymes involved in amino acid biosynthetic pathways. Immobilized PLP is a valuable tool to isolate unknown PLP-dependent enzymes in nature or to perform in vitro selection or directed evolution on …

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Nanodroplet-based reagent delivery into water-in-fluorinated-oil droplets

Bo Zhu, Zhe Du, Yancen Dai, Tetsuya Kitguchi et autres

In vitro compartmentalization is a technique for generating water-in-oil microdroplets to establish the genotype (DNA information)-phenotype (biomolecule function) linkage required by many biological applications. Recently, fluorinated oils have become more widely used for making microdroplets due to their better biocompatibility. However, it …

jp, cn, us (code pays fourni par la source)

Nanodroplet-based reagent delivery into water-in-fluorinated-oil droplets

Bo Zhu, Zhe Du, Yancen Dai, Tetsuya Kitguchi et autres

In vitro compartmentalization is a technique for generating water-in-oil microdroplets to establish the genotype (DNA information)-phenotype (biomolecule function) linkage required by many biological applications. Recently, fluorinated oils have become more widely used for making microdroplets due to their better biocompatibility. However, it …

jp, cn, us (code pays fourni par la source)

Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets

博 朱, Bo Zhu, Zhe Du, Yancen Dai et autres

Cell Survival Enabled by Leakage of a Labile Metabolic Intermediate

Encarnación Medina‐Carmona, Luis I. Gutierrez-Rus, Fadia Manssour-Triedo, Matilda Newton et autres

Many metabolites are generated in one step of a biochemical pathway and consumed in a subsequent step. Such metabolic intermediates are often reactive molecules which, if allowed to freely diffuse in the intracellular milieu, could lead to undesirable side reactions and even …

es, gb, us (code pays fourni par la source)