S. Ledda

m6A RNA methylation in mammalian oocytes and preimplantation embryos: implications for developmental competence

Chiara Cosseddu, Sara Succu, Francesca Mossa, Adele Frau et autres

Chemical modifications of RNA molecules, collectively referred to as epitranscriptomics, have emerged as a critical layer of post-transcriptional gene regulation. Among these modifications, N6-methyladenosine (m6A) plays a key role in modulating RNA stability, localization, and translational efficiency without altering the underlying nucleotide …

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The emerging role of the oocyte cortical domain in maturation, fertilization, and development

Giovanni Coticchio, Valentina Casciani, Laura Francesca Rienzi, Danilo Cimadomo et autres

The cell cortex, a cytoskeletal network with regulatory signalling pathways, is localized beneath the cell membrane: it is especially prominent in mammalian oocytes. As in other cells, the cortex ensures appropriate shape and robustness of oocytes. It is also involved in other …

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Evaluating the Response to Cryopreservation of Ovine Fibroblast Spheroids

Davide Piras, Federico Olia, Chiara Cosseddu, Daniela Bebbere et autres

Cell spheroids are widely studied for their potential applications in tissue engineering and regenerative medicine. The present work investigated the effects of cryopreservation on spheroids derived from ovine fibroblasts, depending on spheroid size (140 or 220 µm). Specifically, it explored how cryopreservation …

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Microplastic exposure induces epithelial barrier alterations and increases collagen deposition in a 3D human endometrial model in vitro

Sharon Arcuri, Georgia Pennarossa, Daniela Bebbere, F. Gandolfi et autres

PURPOSE: To investigate the effects of microplastics (MPs) on the human endometrium in vitro. METHODS: A predictive 3D endometrial in vitro model was generated using highly porous scaffolds where human endometrial stromal (hESC) and epithelial (hEEC) cells were co-cultured for 35 days. …

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m6A RNA methylation dynamics during in vitro maturation of cumulus-oocyte complexes derived from adult or prepubertal sheep

Chiara Cosseddu, Sara Succu, Adele Frau, Francesca Mossa et autres

PURPOSE: N6-methyladenosine (m6A) is the most prevalent base epigenetic modification within eukaryotic mRNAs. It participates in post-transcriptional regulation, including maternal RNA maintenance and decay in mouse oocytes and during maternal-to-zygotic transition. The landscape in other mammalian species remains largely unexplored. The present …

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Correction to: Combination of epigenetic erasing and mechanical cues to generate human epiBlastoids from adult dermal fibroblasts

Georgia Pennarossa, Sharon Arcuri, Teresina De Iorio, S. Ledda et autres

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082 Effect of dietary by-pass linseed oil on embryo, luteal and uterine gene expression linked to prostaglandin synthesis during maternal recognition of pregnancy in Sarda ewes

I. Contreras‐Solís, Daniela Bebbere, Chiara Cosseddu, Cristian Porcu et autres

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Combination of epigenetic erasing and mechanical cues to generate human epiBlastoids from adult dermal fibroblasts

Georgia Pennarossa, Sharon Arcuri, Teresina De Iorio, S. Ledda et autres

Abstract Purpose This study is to develop a new protocol that combines the use of epigenetic cues and mechanical stimuli to assemble 3D spherical structures, arbitrarily defined “epiBlastoids,” whose phenotype is remarkably similar to natural embryos. Methods A 3-step approach is used …

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Fetal Growth and Osteogenesis Dynamics during Early Development in the Ovine Species

Sara Succu, Efisiangelo Contu, Daniela Bebbere, Sergio Domenico Gadau et autres

Increased knowledge of the developmental processes during gestation could provide valuable information on potential alterations in embryonic/fetal development. We examined the development of ovine conceptus between the 20th and 70th day of gestation with three convergent analyses: (1) uterus ultrasound examination and …

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Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts

Sharon Arcuri, Georgia Pennarossa, S. Ledda, F. Gandolfi et autres

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ET-Technologien bei kleinen Wiederkäuern

S. Ledda, Antonio Gonzalez‐Bulnes

Embryo biotechnologies in sheep: Achievements and new improvements

Laura Falchi, S. Ledda, Maria Teresa Zedda

To date, large-scale use of multiple ovulation and embryo transfer (MOET) programmes in ovine species is limited due to unpredictable results and high costs of hormonal stimulation and treatment. Therefore, even if considered reliable, they are not fully applicable in large-scale systems. …

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