Emma R. Holden

Disruption of efflux activity reduces biofilm formation through multiple pathways

Emma R. Holden, Leanne P. Sims, Katie Orchard, Eleftheria Trampari et autres

Free-swimming bacteria must undergo large-scale changes in gene expression to form structured, aggregated biofilm communities. These regulatory changes are susceptible to environmental stimuli such as exposure to antimicrobials, which can affect adhesion, biofilm matrix production, pathogenicity and multidrug susceptibility. Previously, we found …

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Identification of genes important for response of Pseudomonas aeruginosa biofilms to ciprofloxacin exposure

Meng Wang, Emma R. Holden, Muhammad R Yasir, Sarah Bastkowski et autres

Abstract Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe infections in immunocompromised individuals, such as patients with cystic fibrosis where it commonly forms biofilms. Ciprofloxacin is used extensively to treat P. aeruginosa infections, but its effectiveness can be significantly reduced …

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Whole genome sequencing of Salmonella enterica serovar Typhimurium directed evolution experiment, involved continuous exposure of planktonic cells or biofilms to subinhibitory concentrations of acriflavine or phenylalanine-arginine β-naphthylamide (PABN)

Emma R. Holden

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Central metabolism limits planktonic survival but is essential in biofilms during combined limonene and heat treatment of Salmonella

Emma R. Holden, Silvia Guillén, Muhammad Yasir, Natalia Pérez‐Moral et autres

Pivotal plasmids drive the global spread of CTX-M-27 in Escherichia coli

Yan Zhang, Yexin Lin, Jintao Yang, Emma R. Holden et autres

The detection rate of CTX-M-27-producing E. coli has increased worldwide in recent years although relatively little is known about the strains and vectors responsible for this increased isolation. To explore the evolution of CTX-M-27-producing E. coli in the past 20 years at …

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Common food preservatives induce an oxidative stress response in Salmonella enterica serovar Typhimurium

Emma R. Holden, J. Horton, Mark Webber

Despite their frequent use, the mechanisms of action of common food preservatives are poorly understood. As there is a drive to develop alternative preservatives, understanding the mechanisms of action of current preservatives can inform the development of novel food preservatives to ensure …

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Tazobactam selects for multidrug resistance

Emma R. Holden, Muhammad Yasir, A. Keith Turner, Ian G. Charles et autres

Piperacillin-Tazobactam is a β-lactam/β-lactamase inhibitor combination that is amongst the most prescribed antimicrobials in hospital medicine. Piperacillin is inactivated by many common beta-lactamases, but tazobactam inhibits these, allowing successful treatment. The effect of piperacillin on Gram-negative bacteria has been widely studied, but …

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Identification of pathways required for Salmonella to colonize alfalfa using TraDIS- Xpress

Emma R. Holden, Justin Abi Assaf, Haider Al-Khanaq, Noémie Vimont et autres

ABSTRACT Enteropathogenic bacteria, such as Salmonella , have been linked to numerous fresh produce outbreaks, posing a significant public health threat. The ability of Salmonella to persist on fresh produce for extended periods is partly attributed to its capacity to form biofilms, …

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Differential development of antibiotic resistance and virulence between Acinetobacter species

Elizabeth M. Darby, Robert A. Moran, Emma R. Holden, Theresa Morris et autres

ABSTRACT The two species that account for most cases of Acinetobacter -associated bacteremia in the United Kingdom are Acinetobacter lwoffii , often a commensal but also an emerging pathogen, and Acinetobacter baumannii , a well-known antibiotic-resistant species. While these species both cause …

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Common food preservatives impose distinct selective pressures on Salmonella Typhimurium planktonic and biofilm populations

Justin Abi Assaf, Emma R. Holden, Eleftheria Trampari, Mark Webber

Food preservatives are crucial in controlling microbial growth in processed foods to maintain food safety. Bacterial biofilms pose a threat in the food chain by facilitating persistence on a range of surfaces and food products. Cells in a biofilm are often highly …

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Collateral sensitivity increases the efficacy of a rationally designed bacteriophage combination to control Salmonella enterica

Luke Acton, Hannah V. Pye, Gaëtan Thilliez, Rafał Kolenda et autres

ABSTRACT The ability of virulent bacteriophages to lyse bacteria influences bacterial evolution, fitness, and population structure. Knowledge of both host susceptibility and resistance factors is crucial for the successful application of bacteriophages as biological control agents in clinical therapy, food processing, and …

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Genomic characterization of Pseudomonas spp. on food: implications for spoilage, antimicrobial resistance and human infection

Samuel J. Bloomfield, Raphaёlle Palau, Emma R. Holden, Mark Webber et autres

BACKGROUND: Pseudomonas species are common on food, but their contribution to the antimicrobial resistance gene (ARG) burden within food or as a source of clinical infection is unknown. Pseudomonas aeruginosa is an opportunistic pathogen responsible for a wide range of infections and …

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