Fabiola Mascanzoni

Development and characterization of a membrane-permeant GRASP65-mimetic peptide that inhibits Golgi unlinking and cell cycle progression

Romina Inès Cervigni, Raffaella Bonavita, Maria Luisa Barretta, Daniela Spano et autres

• Golgi ribbon disassembly is crucial for mitosis and plays an important, but not yet understood, role in cancer, aging, and neurodegeneration. • Approaches for block disassembly are lacking. • Phosphorylation of the Golgi protein GRASP65 is crucial for Golgi disassembly. • …

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The role of Golgi complex proteins in cell division and consequences of their dysregulation

Roberta Iannitti, Fabiola Mascanzoni, Antonino Colanzi, Daniela Spano

The GC (Golgi complex) plays a pivotal role in the trafficking and sorting of proteins and lipids until they reach their final destination. Additionally, the GC acts as a signalling hub to regulate a multitude of cellular processes, including cell polarity, motility, …

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The Golgi checkpoint: Golgi unlinking during G2 is necessary for spindle formation and cytokinesis

Fabiola Mascanzoni, Inmaculada Ayala, Roberta Iannitti, Alberto Luini et autres

Entry into mitosis requires not only correct DNA replication but also extensive cell reorganization, including the separation of the Golgi ribbon into isolated stacks. To understand the significance of pre-mitotic Golgi reorganization, we devised a strategy to first block Golgi segregation, with …

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The Golgi checkpoint: Golgi unlinking during G2 is required for correct spindle formation and cytokinesis

Fabiola Mascanzoni, Inmaculada Ayala, Roberta Iannitti, Alberto Luini et autres

Abstract The decision to enter mitosis requires not only the control of DNA replication but also additional and crucial preparatory steps such as, for example, partial disassembly of the Golgi complex during G2. The Golgi complex is fundamental for the processing and …

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Functional Coordination among the Golgi Complex, the Centrosome and the Microtubule Cytoskeleton during the Cell Cycle

Fabiola Mascanzoni, Roberta Iannitti, Antonino Colanzi

The Golgi complex of mammalian cells is organized in a ribbon-like structure often closely associated with the centrosome during interphase. Conversely, the Golgi complex assumes a fragmented and dispersed configuration away from the centrosome during mitosis. The structure of the Golgi complex …

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Design, Optimization, and Structural Characterization of an Apoptosis-Inducing Factor Peptide Targeting Human Cyclophilin A to Inhibit Apoptosis Inducing Factor-Mediated Cell Death

Luigi Russo, Fabiola Mascanzoni, Biancamaria Farina, Amalia M. Dolga et autres

Blocking the interaction between the apoptosis-inducing factor (AIF) and cyclophilin A (CypA) by the AIF fragment AIF(370–394) is protective against glutamate-induced neuronal cell death and brain injury in mice. Starting from AIF(370–394), we report the generation of the disulfide-bridged and shorter variant …

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Design, synthesis, structural analysis and biochemical studies of stapled AIF(370-394) analogues as ligand of CypA

Alessandra Monti, Mattia Sturlese, Andrea Caporale, Jessica de Almeida Roger et autres

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The Golgi ribbon: mechanisms of maintenance and disassembly during the cell cycle

Inmaculada Ayala, Fabiola Mascanzoni, Antonino Colanzi

The Golgi complex (GC) has an essential role in the processing and sorting of proteins and lipids. The GC of mammalian cells is composed of stacks of cisternae connected by membranous tubules to create a continuous network, the Golgi ribbon, whose maintenance …

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Organelle Inheritance Control of Mitotic Entry and Progression: Implications for Tissue Homeostasis and Disease

Fabiola Mascanzoni, Inmaculada Ayala, Antonino Colanzi

The Golgi complex (GC), in addition to its well-known role in membrane traffic, is also actively involved in the regulation of mitotic entry and progression. In particular, during the G2 phase of the cell cycle, the Golgi ribbon is unlinked into isolated …

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Binding mode of AIF(370–394) peptide to CypA: insights from NMR, label-free and molecular docking studies

Biancamaria Farina, Mattia Sturlese, Fabiola Mascanzoni, Andrea Caporale et autres

The complex formation between the proteins apoptosis-inducing factor (AIF) and cyclophilin A (CypA) following oxidative stress in neuronal cells has been suggested as a main target for reverting ischemia-stroke damage. Recently, a peptide encompassing AIF residues 370–394 has been developed to target …

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Structural and biochemical insights of CypA and AIF interaction

Biancamaria Farina, Gianluigi Di Sorbo, Angela Chambery, Andrea Caporale et autres

The Cyclophilin A (CypA)/Apoptosis Inducing Factor (AIF) complex is implicated in the DNA degradation in response to various cellular stress conditions, such as oxidative stress, cerebral hypoxia-ischemia and traumatic brain injury. The pro-apoptotic form of AIF (AIF(Δ1-121)) mainly interacts with CypA through …

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FRET-Protease-Coupled Peptidyl-Prolyl cis-trans Isomerase Assay

Andrea Caporale, Fabiola Mascanzoni, Biancamaria Farina, Mattia Sturlese et autres

In this work, a sensitive and convenient protease-based fluorimetric high-throughput screening (HTS) assay for determining peptidyl-prolyl cis-trans isomerase activity was developed. The assay was based on a new intramolecularly quenched substrate, whose fluorescence and structural properties were examined together with kinetic constants …

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