Shota Okuno

Extracellular Volume Remodeling After Transcatheter Aortic Valve Implantation: Factors Associated With Indexed Extracellular Volume Worsening

Yutaka Matsuhiro, Daisuke Nakamura, Seiko Ide, Naoko Higashino et autres

BACKGROUND: Data remain limited regarding whether extracellular volume (ECV) measured by computed tomography (CT) changes after transcatheter aortic valve implantation (TAVI), and which clinical factors may be associated with its temporal evolution. AIMS: The aim of this study was to evaluate changes …

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Coronary intimal proliferation despite lipid reduction during the first year after heart transplantation

Tatsuya Shiraki, Tomohito Ohtani, Isamu Mizote, Daisuke Nakamura et autres

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Long-term prognosis after acute coronary syndrome due to de novo coronary artery lesions and stent thrombosis in patients on hemodialysis

Sho Nakao, Takayuki Ishihara, Katsuki Okada, Hirota Kida et autres

Percutaneous coronary intervention (PCI) in patients with acute coronary syndrome (ACS) undergoing hemodialysis (HD) remains challenging, with limited long-term outcome data. We investigated the long-term prognosis of ACS due to de novo coronary artery lesions and stent thrombosis (ST) in patients with …

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Impact of Elevated Preprocedural Left Ventricular Filling Pressure on Prognosis of Mild Paravalvular Regurgitation Following Transcatheter Aortic Valve Replacement

Shumpei Kosugi, Isamu Mizote, Daisuke Nakamura, Hiroki Sugae et autres

BACKGROUND: Paravalvular regurgitation (PVR) following transcatheter aortic valve replacement (TAVR) is a complication linked to poor outcomes. The prognostic impact of mild PVR, particularly in patients with elevated preprocedural left ventricular (LV) filling pressure, remains uncertain. AIMS: This study aimed to assess …

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Comparison of Vessel Responses Following Combined Sirolimus-Eluting and Endothelial Progenitor Cell Stent and Ultra-Thin Sirolimus-Eluting Stent Implantation by Serial Optical Coherence Tomography and Coronary Angioscopy: A Multicenter Observational Study

Daisuke Nakamura, Isamu Mizote, Takayuki Ishihara, Yutaka Matsuhiro et autres

A dual-therapy sirolimus-eluting and CD34+ antibody-coated Combo Stent (DTS) has been developed to enhance endothelization and capture endothelial progenitor cells; however, vessel responses following DTS implantation remain unclear. Therefore, we evaluated early- and mid-term intravascular characteristics of DTS using intravascular imaging modalities. …

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Long‐Term Bioprosthetic Valve Durability After Transcatheter Aortic Valve Replacement With Supra‐Annular Self‐Expanding Versus Intra‐Annular Balloon‐Expandable Valves in Patients With a Small Aortic Annulus

Yutaka Matsuhiro, Isamu Mizote, Daisuke Nakamura, Tomoharu Dohi et autres

BACKGROUND: The long-term valve durability of supra-annular self-expanding valves (SEV) and intra-annular balloon-expandable valves (BEV) in patients with small aortic annuli remains unexplored. AIMS: This study aimed to determine the long-term bioprosthetic valve durability with SEV versus BEV in patients with small …

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SARS-CoV-2 spike receptor-binding domain is internalized and promotes protein ISGylation in human induced pluripotent stem cell-derived cardiomyocytes

Shota Okuno, Shuichiro Higo, Takumi Kondo, Mikio Shiba et autres

Although an increased risk of myocarditis has been observed after vaccination with mRNA encoding severe acute respiratory syndrome coronavirus 2 spike protein, its underlying mechanism has not been elucidated. This study investigated the direct effects of spike receptor-binding domain (S-RBD) on human …

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Modeling Reduced Contractility and Stiffness Using iPSC-Derived Cardiomyocytes Generated From Female Becker Muscular Dystrophy Carrier

Satoshi Kameda, Shuichiro Higo, Mikio Shiba, Takumi Kondo et autres

Study investigators encountered a female Becker muscular dystrophy (BMD) carrier with advanced heart failure (HF) and identified a stop-gain variant in procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3) as a potential second-hit variant. Isogenic induced pluripotent stem cells (iPSCs) with dominant expression of WT-DMD, …

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Impact of intravascular ultrasound parameters and platelet reactivity on primary patency after drug-coated balloon angioplasty for femoropopliteal artery disease

Shota Okuno, Osamu Iida, Mitsuyoshi Takahara, Yosuke Hata et autres

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Multiplexed measurement of cell type-specific calcium kinetics using high-content image analysis combined with targeted gene disruption

Tomoka Tabata, Yuki Masumura, Shuichiro Higo, Suzuka Kunimatsu et autres

Kinetic analysis of intracellular calcium (Ca2+) in cardiomyocytes is commonly used to determine the pathogenicity of genetic mutations identified in patients with dilated cardiomyopathy (DCM). Conventional methods for measuring Ca2+ kinetics target whole-well cultured cardiomyocytes and therefore lack information concerning individual cells. …

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Human-Induced Pluripotent Stem Cell–Derived Cardiomyocyte Model for TNNT2 Δ160E-Induced Cardiomyopathy

Takumi Kondo, Shuichiro Higo, Mikio Shiba, Yasuaki Kohama et autres

Background: The Δ160E mutation in TNNT2 , which encodes troponin T, is a rare pathogenic variant identified in patients with hypertrophic cardiomyopathy and is associated with poor prognosis. Thus, a convenient human model recapitulating the pathological phenotype caused by TNNT2 Δ160E is …

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Modeling reduced contractility and impaired desmosome assembly due to plakophilin-2 deficiency using isogenic iPS cell-derived cardiomyocytes

Hiroyuki Inoue, Satoki Nakamura, Shuichiro Higo, Mikio Shiba et autres

Loss-of-function mutations in PKP2, which encodes plakophilin-2, cause arrhythmogenic cardiomyopathy (AC). Restoration of deficient molecules can serve as upstream therapy, thereby requiring a human model that recapitulates disease pathology and provides distinct readouts in phenotypic analysis for proof of concept for gene …

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