Aram M. de Haas

Rational design of FVIII sialylated peptides to target Siglec-3 and Siglec-9 and improve peptide formulations for reverse vaccines

Eleonora Nardini, Brigitte-Carole Keumatio Doungstop, Tania Gerpe-Amor, Aram M. de Haas et autres

Reverse vaccine formulations have shown their potential for the treatment of allergies and other autoimmune diseases by the design of antigens that modify dendritic cell function towards tolerogenic responses. We here demonstrate that modification of an immunodominant peptide from factor VIII (FVIII) …

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Vaccination with DC-SIGN-Targeting αGC Liposomes Leads to Tumor Control, Irrespective of Suboptimally Activated T-Cells

Aram M. de Haas, Dorian A. Stolk, Sjoerd T. T. Schetters, Laura Goossens-Kruijssen et autres

Cancer vaccines have emerged as a potent strategy to improve cancer immunity, with or without the combination of checkpoint blockade. In our investigation, liposomal formulations containing synthetic long peptides and α-Galactosylceramide, along with a DC-SIGN-targeting ligand, Lewis Y (LeY), were studied for …

nl, pl, sg (code pays fourni par la source)

Pancreatic cancer-associated fibroblasts modulate macrophage differentiation via sialic acid-Siglec interactions

Kelly Boelaars, Ernesto Rodríguez, Zowi R. Huinen, Chang Liu et autres

Despite recent advances in cancer immunotherapy, pancreatic ductal adenocarcinoma (PDAC) remains unresponsive due to an immunosuppressive tumor microenvironment, which is characterized by the abundance of cancer-associated fibroblasts (CAFs). Once identified, CAF-mediated immune inhibitory mechanisms could be exploited for cancer immunotherapy. Siglec receptors …

nl, us, it, ca (code pays fourni par la source)

Unraveling the impact of sialic acids on the immune landscape and immunotherapy efficacy in pancreatic cancer

Kelly Boelaars, Laura Goossens-Kruijssen, Di Wang, Charlotte M. de Winde et autres

Background Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Despite the successful application of immune checkpoint blockade in a range of human cancers, immunotherapy in PDAC remains unsuccessful. PDAC is characterized by a desmoplastic, hypoxic and highly immunosuppressive tumor microenvironment …

nl (code pays fourni par la source)

Uptake Kinetics Of Liposomal Formulations of Differing Charge Influences Development of in Vivo Dendritic Cell Immunotherapy

Noémi Anna Nagy, Charlotte Castenmiller, Fernando Lozano Vigario, Rinske Sparrius et autres

Dendritic cells (DCs) control adaptive immunity and are therefore attractive for in vivo targeting to either induce immune activation or tolerance, depending on disease. Liposomes, nanoparticles comprised of a lipid bi-layer, provide a nanoplatform for loading disease-relevant antigen, adjuvant and DC-targeting molecules …

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Therapeutic Liposomal Vaccines for Dendritic Cell Activation or Tolerance

Noémi Anna Nagy, Aram M. de Haas, Teunis B. H. Geijtenbeek, Ronald van Ree et autres

Dendritic cells (DCs) are paramount in initiating and guiding immunity towards a state of activation or tolerance. This bidirectional capacity of DCs sets them at the center stage for treatment of cancer and autoimmune or allergic conditions. Accordingly, many clinical studies use …

nl (code pays fourni par la source)

Quantitative Phosphoproteomic Analysis Reveals Dendritic Cell- Specific STAT Signaling After α2-3–Linked Sialic Acid Ligand Binding

Rui-Jún Eveline Li, Aram M. de Haas, Ernesto Rodríguez, Hakan Kalay et autres

Dendritic cells (DCs) are key initiators of the adaptive immunity, and upon recognition of pathogens are able to skew T cell differentiation to elicit appropriate responses. DCs possess this extraordinary capacity to discern external signals using receptors that recognize pathogen-associated molecular patterns. …

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Human C-Type Lectins, MGL, DC-SIGN and Langerin, Their Interactions With Endogenous and Exogenous Ligand Patterns

Fabrizio Chiodo, Aram M. de Haas, Sandra J. van Vliet, Yvette van Kooyk

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γ9δ2T cell diversity and the receptor interface with tumor cells

Anna Vyborova, Dennis X. Beringer, Domenico Fasci, Froso Karaiskaki et autres

γ9δ2T cells play a major role in cancer immune surveillance, yet the clinical translation of their in vitro promise remains challenging. To address limitations of previous clinical attempts using expanded γ9δ2T cells, we explored the clonal diversity of γ9δ2T cell repertoires and …

nl, fr, us (code pays fourni par la source)

Lipo-Based Vaccines as an Approach to Target Dendritic Cells for Induction of T- and iNKT Cell Responses

Dorian A. Stolk, Aram M. de Haas, Jana Vree, Sanne Duinkerken et autres

In this study we developed a liposome-based vaccine containing palmitoylated synthetic long peptides (SLP) and alpha galactosylceramide (αGC) to specifically target dendritic cells (DC) for activation of both innate (invariant natural killer T-cells [iNKT])and adaptive (CD8+ T-cells) players of the immune system. …

nl (code pays fourni par la source)

Aspergillus niger Spores Are Highly Resistant to Space Radiation

Marta Cortesão, Aram M. de Haas, Rebecca Unterbusch, Akira Fujimori et autres

The filamentous fungus Aspergillus niger is one of the main contaminants of the International Space Station (ISS). It forms highly pigmented, airborne spores that have thick cell walls and low metabolic activity, enabling them to withstand harsh conditions and colonize spacecraft surfaces. …

de, jp (code pays fourni par la source)

Fungi in space: Implications for astronaut health and planetary protection

Marta Cortesão, Aram M. de Haas, Michael Laue, Ruth Hemmersbach et autres

Aspergillus and Penicillium were the predominant fungal genera detected aboard the Russian Space Station (Mir) as well as the International Space Station (ISS), and fungal growth has been shown to promote biodegradation of spacecraft materials which might compromise life-support systems [1-2]. Moreover, …