Takashi Ito

Strategic enhancement of Cas9 nickase-driven gene amplification

Hiroaki Takesue, Satoshi Okada, Takashi Ito

Gene amplification plays a critical role in evolution and disease and is widely utilized to overexpress valuable gene products in biotechnology. To broaden these applications, we previously developed break-induced replication (BIR)-mediated tandem repeat expansion (BITREx), a method utilizing Cas9 nickase (nCas9) to …

jp (code pays fourni par la source)

Transcriptional capacity limits copper resistance in yeast harboring highly expanded CUP1 arrays

Hiroaki Takesue, Satoshi Okada, Takashi Ito

Copper resistance in the budding yeast Saccharomyces cerevisiae is primarily mediated by the tandemly arrayed metallothionein gene CUP1. We analyzed eight isogenic strains harboring CUP1 arrays of varying lengths, including those artificially expanded beyond their natural ranges. CUP1 mRNA levels and copper …

High extracellular osmolarity promotes yeast thermotolerance through osmotic modulation and glycerol-dependent adaptation

Saki Oyama, Kosuke Shiraishi, Satoshi Okada, Fumiyoshi Abe et autres

ABSTRACT High-temperature stress is a major constraint on yeast growth and fermentation, and has traditionally been interpreted primarily in terms of intracellular molecular damage such as protein denaturation and aggregation. Despite this extensive focus on intracellular mechanisms, how physical factors within the …

jp (code pays fourni par la source)

Transomic analysis reveals DNA methylation and transcription factor roles in obese liver protein expression

Hideki Maehara, Atsushi Hatano, Masaki Shirai, Toshiya Kokaji et autres

Obesity impairs hepatic functions through abnormal functional protein expression, potentially through DNA methylation, which suppresses gene expression, and changes in transcription factors (TFs) expression. However, the specific protein expression changes associated with DNA methylation in the obese liver remain unclear. To dissect …

jp (code pays fourni par la source)

Sox4 in Treg Cells Suppresses IL-10 Production via c-Maf Degradation and Exacerbates Type 2 Inflammation

Yuki Hayashi, Kensuke Suga, Akira Suto, Arifumi Iwata et autres

SUMMARY The anti-inflammatory cytokine interleukin-10 (IL-10) produced by regulatory T (Treg) cells plays a crucial role in regulating allergic airway inflammation. However, the mechanisms governing IL-10 production in Treg cells remain unclear. In this study, we first identified Sox4 as a gene …

jp, us (code pays fourni par la source)

Usp21 Knockout Causes Abnormal Lipid Metabolism in Mouse and Its Polymorphism Correlates with Hypercholesterolemia in Outpatients

Sailakshmi Iyer, Naoko Hattori, Hiroshi Okuda, Takeya Nakagawa et autres

Usp21, a member of the ubiquitin protease family, plays a vital role in various biological functions. However, the effects of Usp21 dysfunction remain incompletely understood. In this study, we generated Usp21 knockout (KO) mice. Blood tests showed no impairment of liver function …

jp (code pays fourni par la source)

LOBSTERS: a modular vector series with a diverse set of Cas nucleases and plasmid selection markers for multiplex genome editing in Saccharomyces cerevisiae

Satoshi Okada, Emiko Kusumoto, Goro Doi, Shitomi Nakagawa et autres

Abstract The budding yeast Saccharomyces cerevisiae is a central model organism in genetics and synthetic biology, yet efficient multiplex genome editing remains difficult because many toolkits are restricted by limited plasmid selection markers and reduced efficiency when targeting multiple loci. In our …

jp (code pays fourni par la source)

DNA methylation protects cancer cells against senescence

Xiaoying Chen, Kosuke Yamaguchi, Brianna Rodgers, Delphine Goehrig et autres

Inhibitors of DNA methylation such as 5-aza-deoxycytidine are widely used in experimental and clinical settings. However, their mechanism of action is such that DNA damage inevitably co-occurs with loss of DNA methylation, making it challenging to discern their respective effects. Here we …

fr, jp (code pays fourni par la source)

Static and Dynamic Stability Analysis of the Erkenek Twin Tunnels Damaged by the February 6, 2023, Earthquake in Türkiye

Ömer Aydan, N. Malistani, Takashi Ito, Reşat Ulusay et autres

ABSTRACT: On February 6, 2023, two successive devastating earthquakes struck the Kahramanmaraş province in southern Türkiye. The first earthquake with a magnitude of Mw 7.8 occurred nearby Pazarcık, followed by a second Mw 7.6 quake in Ekinözü nine hours later. These events …

jp, tr (code pays fourni par la source)

Analyzing the relationship between human thermal environment and human flow toward a walkable city

Yasuhiro Shimazaki, Takashi Ito

. Introduction‘Walkable city’ is a concept for urban design that prioritizes pedestrians and is becoming popular in Japan. This walkability is influenced by the characteristics of the built environment, microclimate, and so on. Because occupant comfort is an influential factor for outdoor …

jp (code pays fourni par la source)

Catalytically inactive Cas9 attenuates DNA end resection: A potential application for region-restricted random mutagenesis

Satoshi Okada, Takashi Ito

Cas9-mediated modulation of end resection at induced DSB sites will have valuable applications in both genome engineering and mechanistic studies.

jp (code pays fourni par la source)

Tracking clusterin expression in hematopoietic stem cells reveals their heterogeneous composition across the life span

Shuhei Koide, Motohiko Oshima, Takahiro Kamiya, Zhiqian Zheng et autres

ABSTRACT: Hematopoietic stem cells (HSCs) exhibit significant age-related phenotypic and functional alterations. Although single-cell technologies have elucidated age-related compositional changes, prospective identification of aging-associated HSC subsets has remained challenging. In this study, using clusterin (Clu)-green fluorescent protein (GFP) reporter mice, we demonstrated …

jp, gb, us (code pays fourni par la source)