High extracellular osmolarity promotes yeast thermotolerance through osmotic modulation and glycerol-dependent adaptation
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Le résumé fourni par la source
ABSTRACT High-temperature stress is a major constraint on yeast growth and fermentation, and has traditionally been interpreted primarily in terms of intracellular molecular damage such as protein denaturation and aggregation. Despite this extensive focus on intracellular mechanisms, how physical factors within the extracellular environment influence yeast thermotolerance remains poorly understood. Here we demonstrate that increases in extracellular osmolarity markedly attenuate growth inhibition under high-temperature conditions in yeast. This protective effect was consistently observed in multiple laboratory and industrial strains of Saccharomyces cerevisiae , as well as in ascomycetous and basidiomycetous yeasts, indicating that osmotic pressure-dependent thermotolerance is a broadly conserved phenomenon. We also found that extracellular osmolarity dynamically increases during growth and then decreases in a diauxic shift-like pattern after growth arrest. At high temperature, the secretion of glucose-derived metabolites decreased, but that of other solutes increased, suggesting that heat stress alters the composition of extracellular solutes contributing to osmolarity. In addition, intracellular glycerol levels increased at high temperature, and this increase was further enhanced under high-osmolarity conditions. Notably, expression of a constitutively active Hog1 mutant exhibited raised intracellular glycerol levels, enhanced nuclear localization of Hog1, and improved growth under high-temperature conditions. Collectively, these findings support a model in which extracellular osmolarity is modulated to avoid excessive intracellular osmolarity under high-temperature conditions, while the intracellular accumulation of glycerol contributes to yeast adaptation at high-temperature. Our results highlight extracellular–intracellular osmotic coordination as an additional physiological layer of high-temperature stress adaptation in yeast.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- High extracellular osmolarity promotes yeast thermotolerance through osmotic modulation and glycerol-dependent adaptation
- Date Crossref
- 23/04/2026
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Akita Prefectural University pays non établi dans la noticeUniversité ou école supérieure
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Kyoto University pays non établi dans la noticeUniversité ou école supérieure
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Kyushu University Department of Biochemistry pays non établi dans la noticeUniversité ou école supérieure
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Yasuda Women's University pays non établi dans la noticeUniversité ou école supérieure
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Aoyama Gakuin University pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Bioresource Science Department of Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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Graduate School of Agriculture Division of Applied Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Science and Engineering Department of Biological Science pays non établi dans la noticeUniversité ou école supérieure
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College of Science and Engineering Department of Chemistry and Biological Science pays non établi dans la noticeUniversité ou école supérieure
Akita Prefectural University, Kyoto University et Department of Biochemistry — Kyushu University, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.