Takeya Nakagawa

The Mediator Complex: From Transcriptional Regulation to Disease Pathogenesis

Sailakshmi Iyer, Takashi Ito, Takeya Nakagawa, Naoko Hattori

The Mediator complex is a central regulator of eukaryotic transcription, functioning as a dynamic molecular interface between gene-specific transcription factors and RNA polymerase II (Pol II). Although its overall architecture and general role in transcription have been extensively reviewed, accumulating genetic, genomic, …

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The Mediator Complex: Progress over the Past Decade

Sailakshmi Iyer, Takashi Ito, Takeya Nakagawa, Naoko Hattori

The Mediator complex is a central regulator of eukaryotic transcription, functioning as a dynamic molecular bridge between gene-specific transcription factors and RNA polymerase II (Pol II). Although decades of research have established its modular architecture and fundamental role in transcriptional control, recent …

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Usp21 Knockout Causes Abnormal Lipid Metabolism in Mouse and Its Polymorphism Correlates with Hypercholesterolemia in Outpatients

Sailakshmi Iyer, Naoko Hattori, Hiroshi Okuda, Takeya Nakagawa et autres

Usp21, a member of the ubiquitin protease family, plays a vital role in various biological functions. However, the effects of Usp21 dysfunction remain incompletely understood. In this study, we generated Usp21 knockout (KO) mice. Blood tests showed no impairment of liver function …

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Corrigendum to “Cigarette smoke, but not novel tobacco vapor products, causes epigenetic disruption and cell apoptosis” [Biochem. Biophys. Rep. 24 (2020) 100865]

Naoko Hattori, Takeya Nakagawa, Mitsuhiro Yoneda, Kaori Nakagawa et autres

[This corrects the article DOI: 10.1016/j.bbrep.2020.100865.].

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Dissecting the Impact of USP21 on Mouse Lipid Metabolism and Identifying Polymorphism-Associated Hypercholesterolemia in Outpatients

Sailakshmi Iyer, Naoko Hattori, Hiroshi Okuda, Takeya Nakagawa et autres

USP21, a member of the ubiquitin protease family, plays a vital role in various biological functions. However, the effects of USP21 dysfunction have yet to be fully understood. Here, we generated Usp21 knockout (KO) mice and conducted microarray to identify genes that …

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Compounds in cigarette smoke induce EGR1 expression via the AHR, resulting in apoptosis and COPD

Naoko Hattori, Takeya Nakagawa, Mitsuhiro Yoneda, Hiromi Hayashida et autres

Chronic obstructive pulmonary disease (COPD) is a major cause of mortality worldwide, and pulmonary epithelial cell apoptosis is regarded as one of the most important factors in its pathogenesis. Here, we examined the molecular mechanisms of apoptosis caused by cigarette smoke (CS). …

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Long noncoding RNAs transcribed downstream of the human β-globin locus regulate β-globin gene expression

Miki Higashi, Tsuyoshi Ikehara, Takeya Nakagawa, Mitsuhiro Yoneda et autres

The five β-like globin genes (ε, Gγ, Aγ, δ and β) at the human β-globin gene locus are known to be expressed at specific developmental stages, although details of the underlying mechanism remain to be uncovered. Here we used an in vitro …

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Nucleosome assembly protein 1 is a regulator of histone H1 acetylation

Mitsuhiro Yoneda, Kiyoshi Yasui, Takeya Nakagawa, Naoko Hattori et autres

Acetylation of histone H1 is generally considered to activate transcription, whereas deacetylation of H1 represses transcription. However, the precise mechanism of the acetylation is unknown. Here, using chromatography, we identified nucleosome assembly protein 1 (NAP-1) as having inhibitory activity against histone H1 …

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The nucleolus from a liquid droplet perspective

Mitsuhiro Yoneda, Takeya Nakagawa, Naoko Hattori, Takashi Ito

The nucleolus is a membrane-less organelle sequestered from the nucleus by liquid droplet formation through a liquid-liquid phase separation (LLPS). It plays important roles in cell homoeostasis through its internal thermodynamic changes. Reversible nucleolar transitions between coalescence and dispersion are dependent on …

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Cigarette smoke, but not novel tobacco vapor products, causes epigenetic disruption and cell apoptosis

Naoko Hattori, Takeya Nakagawa, Mitsuhiro Yoneda, Kaori Nakagawa et autres

Heat-Not-Burn (HNB) products, generating vapor without combusting tobacco leaves, have been developed with the expectation that the number and quantity of chemicals in the vapor of these products would be reduced compared with the smoke from conventional combustible cigarettes. However, whether the …

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miR-222 regulates proliferation of primary mouse hepatocytes in vitro

Miki Higashi, Mitsuhiro Yoneda, Takeya Nakagawa, Masaaki Ikeda et autres

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The MAP3K2-ERK5 pathway upregulates cyclin D1 expression through histone H2A (Thr120) phosphorylation by VRK1

Masanori Kato, Mitsuhiro Yoneda, Yukio Takeshima, Vishwa Jeet Amatya et autres

VRK1 plays a pivotal role in the upregulation of cyclin D1 (also known as CCND1) by phosphorylating histone H2A (Thr120) around its promoter region. However, the pathways or proteins that regulate this activity upstream of VRK1 remain unknown. It has been confirmed …

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