Athanassia Sotiropoulos

Building bridges: Involvement of animal care staff and laboratory technicians in experimental planning and conduct of animal studies for better job satisfaction and science

Fernando Gonzalez-Uarquin, Paulin Jirkof, Bettina Bert, Penny Hawkins et autres

The involvement of non-scientific staff in discussions about animal welfare and scientific quality is essential for biomedical research progress. In this study, we developed a survey to collect the self-perception of animal care staff (ACS) and laboratory technicians about their involvement in …

A call for preregistration of in vitro research

Céline Heinl, Nuno Henrique Franco, Bettina Bert, Katherina Siewert et autres

This article highlights the benefits of preregistration in improving the reliability and transparency of in vitro studies. By preregistering their studies, researchers can make their findings more reproducible, paving the way for medical progress, and accelerating the replacement of animal experiments.

de, pt, ie, ca, it, gr, at, be, gb, kr, fr (code pays fourni par la source)

Author Reply to Peer Reviews of Transient hypoxia followed by progressive reoxygenation is required for efficient skeletal muscle repair through Rev-ERBα modulation

M. Quétin, Audrey Der Vartanian, Christelle Dubois, Juliette Berthier et autres

fr (code pays fourni par la source)

Role of Serum response factor in cell stiffness and muscle cell fusion

Kassandra Kobon, David Ricardo Do Vale Pereira, Atef Asnacios, Pascal Maire et autres

International audience

fr (code pays fourni par la source)

Transient hypoxia followed by progressive reoxygenation is required for efficient skeletal muscle repair through Rev-ERBα modulation

Marie Quétin, Audrey Der Vartanian, Christelle Dubois, Juliette Berthier et autres

SUMMARY Muscle stem cells (MuSCs) are essential for skeletal muscle repair. Following injury, MuSCs reside in low oxygen environments until muscle fibers and vascularization are restablished. The dynamics of oxygen levels during the regenerative process and its impact on muscle repair has …

fr (code pays fourni par la source)

RhoA Is a Crucial Regulator of Myoblast Fusion

Chiara Noviello, Kassandra Kobon, Voahangy Randrianarison-Huetz, Pascal Maire et autres

Satellite cells (SCs) are adult muscle stem cells that are mobilized when muscle homeostasis is perturbed. Here we show that RhoA in SCs is indispensable to have correct muscle regeneration and hypertrophy. In particular, the absence of RhoA in SCs prevents a …

fr (code pays fourni par la source)

A fast Myosin super enhancer dictates muscle fiber phenotype through competitive interactions with Myosin genes

Matthieu Dos Santos, Stéphanie Backer, Frédéric Aurade, Matthew Man-Kin Wong et autres

The contractile properties of adult myofibers are shaped by their Myosin heavy chain isoform content. Here, we identify by snATAC-seq a 42 kb super-enhancer at the locus regrouping the fast Myosin genes. By 4C-seq we show that active fast Myosin promoters interact …

fr, ca, jp (code pays fourni par la source)

RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth

Chiara Noviello, Kassandra Kobon, Léa Delivry, Thomas Guilbert et autres

Adult skeletal muscle is a plastic tissue that can adapt its size to workload. Here, we show that RhoA within myofibers is needed for overload-induced hypertrophy by controlling satellite cell (SC) fusion to the growing myofibers without affecting protein synthesis. At the …

fr (code pays fourni par la source)

A fast Myh super enhancer dictates adult muscle fiber phenotype through competitive interactions with the fast Myh genes

Matthieu Dos Santos, Stéphanie Backer, Frédéric Aurade, Matthew Man-Kin Wong et autres

Summary The contractile properties of adult myofibers are shaped by their Myosin heavy chain (MYH) isoform content. We identify by snATAC-seq a 42kb super-enhancer (SE) at the locus regrouping the fast Myh (f Myh ) genes. By 4C-seq we show that active …

fr, ca, jp (code pays fourni par la source)

RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth

Chiara Noviello, Kassandra Kobon, Léa Delivry, Thomas Guilbert et autres

Summary Adult skeletal muscle is a plastic tissue that can adapt its size to workload. Here, we show that RhoA within myofibers is needed for overload-induced hypertrophy by controlling satellite cell fusion to the growing myofibers without affecting protein synthesis. At the …

fr (code pays fourni par la source)

Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers

Matthieu Dos Santos, Stéphanie Backer, Benjamin Saintpierre, Brigitte Izac et autres

Skeletal muscle fibers are large syncytia but it is currently unknown whether gene expression is coordinately regulated in their numerous nuclei. Here we show by snRNA-seq and snATAC-seq that slow, fast, myotendinous and neuromuscular junction myonuclei each have different transcriptional programs, associated …

fr (code pays fourni par la source)

Role of damage and management in muscle hypertrophy: Different behaviors of muscle stem cells in regeneration and hypertrophy

So‐ichiro Fukada, Takayuki Akimoto, Athanassia Sotiropoulos

Skeletal muscle is a dynamic tissue with two unique abilities; one is its excellent regenerative ability, due to the activity of skeletal muscle-resident stem cells named muscle satellite cells (MuSCs); and the other is the adaptation of myofiber size in response to …

jp, fr (code pays fourni par la source)