Xiaocheng Weng

Conductive Cu-MOF synergizing with enzyme-free catalytic cascade amplification for ultrasensitive electrochemical detection of miRNA-21

Li Huang, Ziyi Liu, Huiyan Cao, 董国芃 et autres

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Photocatalytic Proximity Labeling Maps RNA and Protein Subcellular Landscapes

Meihan Hou, Linao Ren, Xi Xin, Rongjie Zhu et autres

Abstract Mapping the molecular landscapes within subcellular compartments is critical for understanding cellular function. Photocatalytic proximity labeling has emerged as an increasingly diversified chemical strategy for interrogating biomolecular microenvironments in living systems. However, a modular small-molecule platform that can be readily retargeted …

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Competitive biofunctionalized Au@Pt nanoprobe-based electrochemical biosensor for signal-amplified detection of m6A in gastric cancer

Xi Cheng, Huiyan Cao, Li Huang, Yang Ww et autres

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Development of the First YTHDC1 Degrader with Improved Antileukemia Activity

Zhongpao Xie, Yuanyuan Zhang, Weicai Song, Qianqian Qi et autres

Abstract N6-Methyladenosine (m6A) represents the most prevalent internal RNA modification in eukaryotic mRNA, with YTHDC1 serving as a critical nuclear reader protein implicated in acute myeloid leukemia (AML). Although selective inhibitors of YTHDC1 have been reported, whether targeted degradation of YTHDC1 could …

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Simultaneous single-base resolution profiling of 5mC and 5hmC using BRIGHT-seq

Xiaochen Xue, Ziang Lu, Wei Yang, Shaoqing Han et autres

Cytosine modifications in DNA, particularly 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), are key epigenetic marks involved in gene regulation and chromatin organization. Simultaneous and base-resolution detection of 5mC and 5hmC is essential for understanding their interplay in gene regulation. However, existing methods often …

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Solvent-Modulated Orthogonal Release from Covalent Organic Frameworks Enables Sequential Multiomics Enrichment

Yue Wang, Xuejiao Wang, Zihan Cai, Long Yu et autres

The efficient integration of genomics, transcriptomics, and proteomics in liquid biopsy is currently impeded by the lack of materials capable of simultaneous capture and stepwise separation of nucleic acids and proteins. To bridge this gap, we designed a series of covalent organic …

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A 5-hydroxymethylcytosine-based noninvasive model for acute myocardial infarction in type 2 diabetes: implications in cardiovascular outcomes from a 5-year median follow-up study

KUN YU YANG, Shanshan Qin, Shaohua Xu, Xiuji Cui et autres

BACKGROUND: Patients with type 2 diabetes (T2D) are at an increased risk of developing acute myocardial infarction (AMI), yet the role of 5-hydroxymethylcytosines (5hmC) changes in T2D-associated cardiovascular events remains poorly understood. Despite therapeutic advances, patients with T2D who suffer an AMI …

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Molecular medicine driven by nucleic acid modifications: state of the field, challenges, and opportunities

Yan Huang, Ling Zhu, Xiaocheng Weng, Peng Chen et autres

Aberrant nucleic acid epigenetic modifications are closely linked to the initiation and progression of human diseases, positioning this field as a key strategic entry point for advances in molecular medicine. In recent years, under the support of the National Natural Science Foundation …

Nanobody-Tethered Transposition for Dual Profiling of Histone Modification and DNA Methylation

Jizhou Liu, Jindi Zhou, Ziang Lu, Guorun Tang et autres

DNA methylation and histone modifications are critical epigenetic regulators that orchestrate gene expression and modulate various physiological and pathological processes. However, existing methodologies for simultaneous profiling of these epigenetic marks often require high cell input and suffer from data loss due to …

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A gold nanostars-based electrochemical biosensor for sensitive detection of RNA pseudouridine

Li Huang, Xi Cheng, Huiyan Cao, Shouquan Dong et autres

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Targeted detection method for locus-specific m 6 A modifications in low-abundance transcripts based on chemical conversion

Ting Liu, Xin Fang, Yue Lu, Xiumin Liu et autres

In this study, we developed a method for locus-specific m 6 A detection, which employs sodium nitrite and glyoxal-based chemical conversion with probe hybridization.

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Mechanistic Insights into the Borane-Mediated Conversion of 5-Carboxylcytosine to Dihydrouracil in DNA Enable an Efficient Labeling Method Using Alkoxyamines

Cong Ding, Zipeng Wang, Bingwen Li, Jiangjuan Shao et autres

TET-assisted pyridine borane sequencing (TAPS) is a powerful method for mapping cytosine modifications, yet the underlying mechanism of conversion of 5-carboxylcytosine (5caC) to dihydrouracil (DHU) remains poorly understood. Here, through both experimental and computational studies, we show that the reaction proceeds via …

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