Simultaneous single-base resolution profiling of 5mC and 5hmC using BRIGHT-seq

Le résumé fourni par la source

Cytosine modifications in DNA, particularly 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), are key epigenetic marks involved in gene regulation and chromatin organization. Simultaneous and base-resolution detection of 5mC and 5hmC is essential for understanding their interplay in gene regulation. However, existing methods often rely on differential subtraction strategies, making direct and concurrent analysis within a single assay challenging. Here, we present the Base Replacement for Integrated Genome-wide Methylation and Hydroxymethylation Tracking Sequencing (BRIGHT-seq), a sequencing strategy that enables direct and simultaneous detection of 5mC and 5hmC at single-base resolution within the same DNA molecule. By integrating enzymatic and chemical treatments, BRIGHT-seq converts 5mC to adenine and 5hmC to thymine, enabling direct identification of both modifications without relying on differential subtraction. We demonstrate the applicability of BRIGHT-seq by mapping 5mC and 5hmC landscapes across human and mouse genomes. BRIGHT-seq provides a useful and high-resolution tool for epigenetic research, facilitating future studies on the roles of 5mC and 5hmC in gene regulation and disease.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.