Joshua J. Blakeslee

Cytosolic- and membrane-localized oxidized indole-3-acetic acid formation regulates developmental auxin transients

Mark K. Jenness, Reuben Tayengwa, Shiyun Cao, Román Ramos Báez et autres

Catabolism of the auxin indole-3-acetic acid (IAA) to terminate cellular responses primarily occurs in three steps: (i) conjugation of IAA to Asp/Glu, (ii) oxidation of the indole ring by DIOXYGENASE FOR AUXIN OXIDATION (DAO), and (iii) amidohydrolase cleavage of Asp/Glu. This study …

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A robust method for monitoring the growth and metabolism of probiotic bacteria in vitro

Jennifer L. Janovick, Justin A. North, Shiqi Zhang, Jiangjiang Zhu et autres

Lactic acid bacteria are involved in many food, nutritional, and biotechnological applications, where carbohydrates are their main source of energy. Although the study of bacterial growth and metabolism in vitro offers several advantages, protocols detailing optimum conditions for a particular bacterium are …

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Integrated Transcriptomic and Metabolomic Analyses Reveal the Importance of the Terpenoid, Fatty Acid, and Flavonoid Pathways in Rice Cell Death and Defense

Pengfei Bai, Yanfang Liu, Laisa Gomes-Dias, Rachel Combs‐Giroir et autres

Lesion mimic mutants provide unique tools to investigate plant–pathogen interactions, often exhibiting hypersensitive responses in the absence of biotic or abiotic stresses. The overexpression of the S-domain receptor-like kinase gene, SPL11 cell-death suppressor 2 (SDS2), in rice leads to constitutive programmed cell …

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Transcriptomic and Metabolomic Analyses of the Piz-t-Mediated Resistance in Rice against Magnaporthe oryzae

Naeyeoung Choi, Xiao Xu, Pengfei Bai, Yanfang Liu et autres

Magnaporthe oryzae causes devastating rice blast disease, significantly impacting rice production in many countries. Among the many known resistance (R) genes, Piz-t confers broad-spectrum resistance to M. oryzae isolates and encodes a nucleotide-binding site leucine-rich repeat receptor (NLR). Although Piz-t-interacting proteins and …

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Amino Acid Compound 2 (AAC2) Treatment Counteracts Insulin-Induced Synaptic Gene Expression and Seizure-Related Mortality in a Mouse Model of Alzheimer’s Disease

Zhijie Deng, Aejin Lee, Tao Lin, Sagarika Taneja et autres

Diabetes is a major risk factor for Alzheimer’s disease (AD). Amino acid compound 2 (AAC2) improves glycemic and cognitive functions in diabetic mouse models through mechanisms distinct from insulin. Our goal was to compare the effects of AAC2, insulin, and their nanofiber-forming …

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Proteomic and Targeted Lipidomic Analyses of Fluid and Rigid Rubber Particle Membrane Domains in Guayule

Joshua J. Blakeslee, Eun-Hyang Han, Yun Lin, Jinshan Ella Lin et autres

Rubber (cis-1,4-polyisoprene) is produced in cytosolic unilamellar vesicles called rubber particles (RPs), and the protein complex responsible for this synthesis, the rubber transferase (RTase), is embedded in, or tethered to, the membranes of these RPs. Solubilized enzyme activity is very difficult to …

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Stability of Epinephrine in a Normal Saline Solution

Caroline M. Sawicki, Daniel B. McKim, Hongrui Wang, Morgan Vasas et autres

Objective: Dilution of concentrated epinephrine prior to intravenous use during clinical emergencies can delay urgent interventions. The objective of this study was to determine whether diluted epinephrine remains stable and sterile over time in common hospital settings. Methods: Epinephrine samples were prepared …

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Identification and quantification of pigments in plant leaves using thin layer chromatography-Raman spectroscopy (TLC-Raman)

Taylor D. Payne, Lily R. Dixon, Fiona C. Schmidt, Joshua J. Blakeslee et autres

Carotenoids are yellow, orange, and red pigments commonly found in plants. In leaves, these molecules are essential for photosynthesis, but they also play a major role in plant growth and development. Efficiently monitoring concentrations of specific carotenoids in plant tissues could help …

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A Robust Method for Monitoring the Growth and Metabolism of Probiotic Bacteria in Vitro

Jennifer L. Janovick, Justin A. North, Shiqi Zhang, Jiangjiang Zhu et autres

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A Robust Method for Monitoring the Growth and Metabolism of Probiotic Bacteria in Vitro

Jennifer L. Janovick, Justin A. North, Shiqi Zhang, Jiangjiang Zhu et autres

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Extractable latex yield from Taraxacum kok-saghyz roots is enhanced by increasing rubber particle buoyancy

Kristóf Molnár, Joshua J. Blakeslee, Colleen Marie McMahan, Aswathy Sasidharan Pillai et autres

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P03-067-23 Deuterium-Labeled Spinach as a Plant Model for Studying Food Matrix Interactions on Vitamin E Bioavailability in Humans

Shahabeddin Rezaei, Sisi Cao, Min Zeng, Maria Antonius et autres