S. Ahn

Protocol for systematic screening of histone H3/H4 mutants to identify histone residues critical for BCAA-dependent replicative lifespan

Sejung Park, Yan Liu, S. Ahn

Here, we present a protocol for high-throughput replicative lifespan (RLS) screening in Saccharomyces cerevisiae using micromanipulation-based aging assays with growth phenotyping. We describe steps for preparing yeast strains, isolating virgin cells, and monitoring divisions to calculate lifespan changes in histone H3/H4 mutants. …

kr (code pays fourni par la source)

Epigenetic regulation of intracellular branched-chain amino acid homeostasis maintains a normal lifespan

Sejung Park, Suji Lim, Hong‐Yeoul Ryu, S. Ahn

restored icBCAA balance and partially rescued lifespan defects in H3K4 or H3K121 mutants. Additionally, H3K4 and H3K121 mutations affected lifespan regulation through TORC1 signaling. Our findings suggest that the epigenetic control of BCAA metabolism, specifically through the modification of histone residues, contributes …

kr (code pays fourni par la source)

Tho2‐mediated escort of Nrd1 regulates the expression of aging‐related genes

Yan Liu, J.Y. Park, Suji Lim, Ruxin Duan et autres

The relationship between aging and RNA biogenesis and trafficking is attracting growing interest, yet the precise mechanisms are unknown. The THO complex is crucial for mRNA cotranscriptional maturation and export. Herein, we report that the THO complex is closely linked to the …

kr (code pays fourni par la source)

Sus1 maintains a normal lifespan through regulation of TREX-2 complex-mediated mRNA export

Suji Lim, Yan Liu, Byung‐Ho Rhie, Chun Kim et autres

Eukaryotic gene expression requires multiple cellular events, including transcription and RNA processing and transport. Sus1, a common subunit in both the Spt-Ada-Gcn5 acetyltransferase (SAGA) and transcription and export complex-2 (TREX-2) complexes, is a key factor in coupling transcription activation to mRNA nuclear …

kr (code pays fourni par la source)

Nuclear mRNA Export and Aging

Hyun-Sun Park, Jongbok Lee, Hyun‐Shik Lee, S. Ahn et autres

The relationship between transcription and aging is one that has been studied intensively and experimentally with diverse attempts. However, the impact of the nuclear mRNA export on the aging process following its transcription is still poorly understood, although the nuclear events after …

kr (code pays fourni par la source)

Sus1 maintains a normal lifespan through regulation of TREX-2 complex-mediated mRNA export

Suji Lim, Yan Liu, Byung‐Ho Rhie, Chun Kim et autres

Abstract Eukaryotic gene expression requires multiple cellular events, including transcription and RNA processing and transport. Sus1, a common subunit in both the Spt-Ada-Gcn5 acetyltransferase (SAGA) and transcription and export complex-2 (TREX-2) complexes, is a key factor in coupling transcription activation to mRNA …

kr (code pays fourni par la source)

Cotranscriptional Set2 Methylation of Histone H3 Lysine 36 Recruits a Repressive Rpd3 Complex

Sean R. Collins, Maya Schuldiner, Stephen Buratowski, Natalie Thompson et autres

SummaryThe yeast histone deacetylase Rpd3 can be recruited to promoters to repress transcription initiation. Biochemical, genetic, and gene-expression analyses show that Rpd3 exists in two distinct complexes. The smaller complex, Rpd3C(S), shares Sin3 and Ume1 with Rpd3C(L) but contains the unique subunits …

Epigenetic Targeting of Histone Deacetylases in Diagnostics and Treatment of Depression

Hyuntae Park, Jongmin Kim, S. Ahn, Hong‐Yeoul Ryu

Depression is a highly prevalent, disabling, and often chronic illness that places substantial burdens on patients, families, healthcare systems, and the economy. A substantial minority of patients are unresponsive to current therapies, so there is an urgent need to develop more broadly …

kr (code pays fourni par la source)

The Spt7 subunit of the SAGA complex is required for the regulation of lifespan in both dividing and nondividing yeast cells

Suji Lim, Hyo‐Jeong Ahn, Ruxin Duan, S. Ahn

kr (code pays fourni par la source)

SUMO and cellular adaptive mechanisms

Hong‐Yeoul Ryu, S. Ahn, Mark Hochstrasser

The ubiquitin family member SUMO is a covalent regulator of proteins that functions in response to various stresses, and defects in SUMO-protein conjugation or deconjugation have been implicated in multiple diseases. The loss of the Ulp2 SUMO protease, which reverses SUMO-protein modifications, …

kr, us (code pays fourni par la source)

Symmetric dimethylation on histone H4R3 associates with histone deacetylation to maintain properly polarized cell growth

Ruxin Duan, Hong‐Yeoul Ryu, S. Ahn

kr (code pays fourni par la source)

Multiple roles of CTDK-I throughout the cell

Rakesh Srivastava, Ruxin Duan, S. Ahn

in, kr (code pays fourni par la source)