Erica Lieberman

Treatment of Shiga toxin–producing E. coli infection by CRISPR-Cas–targeted cleavage of the Shiga toxin gene in animal models

Matthieu Galtier, Antonina O. Krawczyk, Fabien J. Fuche, Loïc H. Charpenay et autres

Escherichia coli is not only a ubiquitous gut commensal but also an opportunistic pathogen responsible for severe intestinal and extraintestinal infections. Shiga toxin–producing E. coli (STEC) poses a notable public health threat, particularly in children, where infections can lead to bloody diarrhea …

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Treatment of STEC infection via CRISPR-Cas targeted cleavage of the Shiga toxin gene in animal models

Matthieu Galtier, Antonina O. Krawczyk, Fabien J. Fuche, Loïc H. Charpenay et autres

Abstract Escherichia coli is a ubiquitous gut commensal but also an opportunistic pathogen responsible for severe intestinal and extra-intestinal infections. Shiga toxin-producing E. coli (STEC) pose a significant public health threat, particularly in children, where infections can lead to bloody diarrhea and …

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Phage–host coevolution in natural populations

Damien Piel, Maxime Bruto, Yannick Labreuche, François Blanquart et autres

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Phages and their satellites encode hotspots of antiviral systems

François Rousset, Florence Depardieu, Solange Miele, Anne-Laure Laval et autres

Bacteria carry diverse genetic systems to defend against viral infection, some of which are found within prophages where they inhibit competing viruses. Phage satellites pose additional pressures on phages by hijacking key viral elements to their own benefit. Here, we show that …

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Genetic determinism of phage-bacteria coevolution in natural populations

Damien Piel, Maxime Bruto, Yannick Labreuche, François Blanquart et autres

ABSTRACT Coevolution between bacteriophage (or phage) and their bacterial host is thought to be key for the coexistence of these antagonists. Recent studies have revealed the major role of mobile genetic elements in the emergence of phage resistant hosts but how phage …

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CRISPR/Cas9-Assisted Transformation-Efficient Reaction (CRATER) for Near-Perfect Selective Transformation

Lynn J. Rothschild, Daniel T. Greenberg, Jack R. Takahashi, Kirsten A. Thompson et autres

The CRISPR (Clustered, Regularly Interspaced, Short Palindromic Repeats)/Cas9 system has revolutionized genome editing by providing unprecedented DNA-targeting specificity. Here we demonstrate that this system can be also applied in vitro to fundamental cloning steps to facilitate efficient plasmid selection for transformation and …

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biOrigami: A New Approach to Reduce the Cost of Space Missions

Charles Calvet, Tyler Devlin, Daniel T. Greenberg, Tina Ju et autres

Space exploration lies at the inquisitive core of human nature, yet high costs hinder the advancement of this frontier. We are harnessing the replicative properties of biology to create biOrigamibiological, self-folding origamito reduce the mass, volume, and assembly time of materials needed …

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A fully decompressed synthetic bacteriophage øX174 genome assembled and archived in yeast

Paul R. Jaschke, Erica Lieberman, Jon Vallejo-Rodríguez, Adrian Sierra et autres

A Disposable Tear Glucose Biosensor—Part 3: Assessment of Enzymatic Specificity

Kenneth Lan, Kenyon McAferty, Pankti Shah, Erica Lieberman et autres

BACKGROUND: A concept for a tear glucose sensor based on amperometric measurement of enzymatic oxidation of glucose was previously presented, using glucose dehydrogenase flavin adenine dinucleotide (GDH-FAD) as the enzyme. Glucose dehydrogenase flavin adenine dinucleotide is further characterized in this article and …

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