Florence Depardieu

Shuttling, swapping, and mixing: the rapid modular evolution of antiviral repertoires in temperate phages and their satellites

Jorge A. Moura de Sousa, Florence Depardieu, Marie Touchon, Raphaël Laurenceau et autres

Interactions between bacteria, bacteriophages, and their satellites are shaped by a myriad of defence and counter-defence mechanisms. Here, we identified and characterized the defence hotspots of thousands of P2-like phages and P4-like satellites to elucidate the origins and evolutionary dynamics of defence …

fr, us (code pays fourni par la source)

OLD sentinel: an abortive tRNase surveys phage replication and DNA defects in RecBCD-compromised cells

Anton Derzhaev, Jing Zhang, Alexey A. Gavrilov, Svetlana Belukhina et autres

OLD, an abortive immunity protein from prophage P2, consists of an ABC ATPase sensor and a TO-PRIM nuclease effector - a core architecture shared by a large protein family, including components of anti-phage systems Gabija, PARIS, Septu, and Lamassu. OLD was originally …

cz, cn, us, fr, ru (code pays fourni par la source)

Shuttling, swapping and mixing: the rapid modular evolution of antiviral repertoires in temperate phages and their satellites

J. De Sousa, Florence Depardieu, Marie Touchon, Raphaël Laurenceau et autres

Abstract Interactions between bacteria, bacteriophages, and their satellites are shaped by a myriad of defence and counter-defence mechanisms. Here, we identified and characterized the defence hotspots of thousands of P2-like phages and P4-like satellites to elucidate the origins and evolutionary dynamics of …

fr (code pays fourni par la source)

A CRISPR-Cas9 method for selectively eliminating plasmids in enterobacteria

Javier DelaFuente, Florence Depardieu, David Bikard, Alvaro San Millan

Specificity and mechanism of tRNA cleavage by the AriB Toprim nuclease of the PARIS bacterial immune system

Svetlana Belukhina, Baptiste Saudemont, Florence Depardieu, Tom Lorthios et autres

Transfer RNA (tRNA) molecules have been recently recognized as widespread targets of bacterial immune systems. Translation inhibition through tRNA cleavage or modification inhibits phage propagation, thereby protecting the bacterial population. To counteract this, some viruses encode their own tRNA molecules, allowing infection …

ru, fr, de (code pays fourni par la source)

Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems

Baptiste Darracq, Eloi Littner, Manon Brunie, Julia Bos et autres

Integrons are genetic systems that drive bacterial adaptation by acquiring, expressing, and shuffling gene cassettes. While mobile integrons are well known for spreading antibiotic resistance genes, the functions of the hundreds of cassettes carried by sedentary integrons remain largely unexplored. We show …

fr, us, ca (code pays fourni par la source)

Specificity and Mechanism of tRNA cleavage by the AriB Toprim nuclease of the PARIS bacterial immune system

Svetlana Belukhina, Baptiste Saudemont, Florence Depardieu, Tom Lorthios et autres

Abstract Transfer RNA molecules have been recently recognized as widespread targets of bacterial immune systems. Translation inhibition through tRNA cleavage or modification inhibits phage propagation, thereby protecting the bacterial population. To counteract this, some viruses encode their own tRNA molecules, allowing infection …

ru, fr, de (code pays fourni par la source)

Author Correction: A virally encoded tRNA neutralizes the PARIS antiviral defence system

Nathaniel Burman, Svetlana Belukhina, Florence Depardieu, Royce A. Wilkinson et autres

In the version of this article initially published, there was a mistake in the RNA blot section of the Methods, where in the text now reading “Following prehybridization … the membrane was hybridized overnight with 2 nM FAM (final concentration) (6-carboxyfluorescein)”, the …

us, ru, fr, se (code pays fourni par la source)

Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system

Dolores L. Guzmán-Herrador, Andrea Fernández-Gómez, Florence Depardieu, David Bikard et autres

CRISPR-associated (Cas) endonucleases and their derivatives are widespread tools for the targeted genetic modification of both prokaryotic and eukaryotic genomes. A critical step of all CRISPR-Cas technologies is the delivery of the Cas endonuclease to the target cell. Here, we investigate the …

es, fr (code pays fourni par la source)

A virally encoded tRNA neutralizes the PARIS antiviral defence system

Nathaniel Burman, Svetlana Belukhina, Florence Depardieu, Royce A. Wilkinson et autres

Viruses compete with each other for limited cellular resources, and some deliver defense mechanisms that protect the host from competing genetic parasites 1 . PARIS is a defense system, often encoded in viral genomes, that is composed of a 55 kDa ABC …

us, ru, fr, se (code pays fourni par la source)

Sedentary chromosomal integrons as biobanks of bacterial anti-phage defence systems

Baptiste Darracq, Eloi Littner, Manon Brunie, Julia Bos et autres

Integrons are genetic systems that accelerate bacterial adaptation by acquiring and shuffling gene cassettes. Mobile integrons spread antibiotic resistance genes among bacteria, while the sedentary chromosomal integrons contain up to hundreds of cassettes of unknown function. Here, we show that many of …

fr, ca (code pays fourni par la source)

An antiplasmid system drives antibiotic resistance gene integration in carbapenemase-producing Escherichia coli lineages

Pengdbamba Dieudonné Zongo, Nicolas Cabanel, Guilhem Royer, Florence Depardieu et autres

Abstract Plasmids carrying antibiotic resistance genes (ARG) are the main mechanism of resistance dissemination in Enterobacterales. However, the fitness-resistance trade-off may result in their elimination. Chromosomal integration of ARGs preserves resistance advantage while relieving the selective pressure for keeping costly plasmids. In …

fr (code pays fourni par la source)