Sara Billings

Epithelial-mesenchymal Wnt crosstalk directs planar cell polarity in the developing cochlea

Ippei Kishimoto, Abel P. David, Kevin Rose, Balasubramanian Narasimhan et autres

Cochlear hair cells are precisely oriented by planar cell polarity. Wnt proteins direct planar cell polarity in vertebrates, but their roles in regulating cochlear planar cell polarity remain unclear. Here, we inhibit Wnt secretion by ablating epithelial Wntless and find shortened cochlea, …

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Benchmarking cell type and gene set annotation by large language models with AnnDictionary

George Crowley, Robert C. Jones, Mark A. Krasnow, Angela Oliveira Pisco et autres

We develop an open-source package called AnnDictionary to facilitate the parallel, independent analysis of multiple anndata. AnnDictionary is built on top of LangChain and AnnData and supports all common large language model (LLM) providers. AnnDictionary only requires 1 line of code to …

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Single-cell transcriptomic atlas reveals increased regeneration in diseased human inner ear balance organs

Tian Wang, Angela H. Ling, Sara Billings, Davood K. Hosseini et autres

Mammalian inner ear hair cell loss leads to permanent hearing and balance dysfunction. In contrast to the cochlea, vestibular hair cells of the murine utricle have some regenerative capacity. Whether human utricular hair cells regenerate in vivo remains unknown. Here we procured …

cn, us (code pays fourni par la source)

Selection of viral capsids and promoters affects the efficacy of rescue of Tmprss3-deficient cochlea

Ksenia A. Aaron, Katja Pekrun, Patrick J. Atkinson, Sara Billings et autres

Adeno-associated virus (AAV)-mediated gene transfer has shown promise in rescuing mouse models of genetic hearing loss, but how viral capsid and promoter selection affects efficacy is poorly characterized. Here, we tested combinations of AAVs and promoters to deliver Tmprss3 , mutations in …

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Single-cell transcriptomic atlas reveals increased regeneration in diseased human inner ears

Tian Wang, Angela H. Ling, Sara Billings, Davood K. Hosseini et autres

Abstract Mammalian inner ear hair cell loss leads to permanent hearing and balance dysfunction. In contrast to the cochlea, vestibular hair cells of the murine utricle have some regenerative capacity. Whether human utricular hair cells regenerate remains unknown. Here we procured live, …

cn, us (code pays fourni par la source)

Gpr125 Marks Distinct Cochlear Cell Types and Is Dispensable for Cochlear Development and Hearing

Haiying Sun, Tian Wang, Patrick J. Atkinson, Sara Billings et autres

The G protein-coupled receptor (GPR) family critically regulates development and homeostasis of multiple organs. As a member of the GPR adhesion family, Gpr125 (Adgra3) modulates Wnt/PCP signaling and convergent extension in developing zebrafish, but whether it is essential for cochlear development in …

hk, cn, us (code pays fourni par la source)

Opposing effects of Wnt/β-catenin signaling on epithelial and mesenchymal cell fate in the developing cochlea

Sara Billings, Nina M. Myers, Lee A. Quiruz, Alan G. Cheng

During embryonic development, the otic epithelium and surrounding periotic mesenchymal cells originate from distinct lineages and coordinate to form the mammalian cochlea. Epithelial sensory precursors within the cochlear duct first undergo terminal mitosis before differentiating into sensory and non-sensory cells. In parallel, …

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Ablation of DHRS3 is associated with alterations in retinoic acid metabolism and signaling and with defects in developmental programming in mice (39.8)

Alexander R. Moise, Sara Billings, Keely Pierzchalski, Naomi Butler Tjaden et autres

Background: The levels of the signaling molecule all‐ trans ‐retinoic acid (ATRA) are tightly controlled at the level of enzymes involved in its synthesis and degradation. Previous studies have implicated the retinaldehyde reductase DHRS3 in establishing a negative feedback mechanism to limit …

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The retinaldehyde reductase DHRS3 is essential for preventing the formation of excess retinoic acid during embryonic development

Sara Billings, Keely Pierzchalski, Naomi E. Butler Tjaden, Xiaoyan Pang et autres

Oxidation of retinol via retinaldehyde results in the formation of the essential morphogen all‐ trans ‐retinoic acid (ATRA). Previous studies have identified critical roles in the regulation of embryonic ATRA levels for retinol, retinaldehyde, and ATRA‐oxidizing enzymes; however, the contribution of retinaldehyde …

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The interaction of ELKS with Ca2+ channel β subunits may contribute to active zone organization

Sara Billings

The cytoskeletal matrix of the active zone (CAZ) and synaptic voltage-dependent calcium channels (VDCCs) are both required for the organization and regulation of synaptic vesicle release. Here we report a novel interaction between the CAZ protein family ELKS and VDCC β subunits. …

ELKS1 and Ca2+ channel subunit β4 interact and colocalize at cerebellar synapses

Sara Billings, Gwenaëlle L. Clarke, Hiroshi Nishimune

The cytoskeletal matrix of the active zone and synaptic voltage-dependent calcium channels (VDCCs) are both necessary components for the organization and regulation of synaptic vesicle release. In this study, we report a novel interaction between the cytoskeletal matrix of the active zone …

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Calcium Channels Link the Muscle-Derived Synapse Organizer Laminin β2 to Bassoon and CAST/Erc2 to Organize Presynaptic Active Zones

Jie Chen, Sara Billings, Hiroshi Nishimune

Synapse formation requires the organization of presynaptic active zones, the synaptic vesicle release sites, in precise apposition to postsynaptic neurotransmitter receptor clusters; however, the molecular mechanisms responsible for these processes remain unclear. Here, we show that P/Q-type and N-type voltage-dependent calcium channels …

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