Liam D. Hurley

Optimizing Oxylipin Analysis with Liquid Chromatography Mass Spectrometry through Bio-Inert Systems and Ion Funnel Adjustments

John T. Shuster, Lingshuang Wu, Jericha Mill, Michaela M. Morhaus et autres

Oxylipins are potent signaling lipids that affect inflammation, vascular tone, and metabolism, making them relevant in many diseases. Oxylipins are measured with liquid chromatography-mass spectrometry (LC-MS), but challenges in quantification arise due to low abundance and rapid degradation. In this study, we …

us (code pays fourni par la source)

Gαz Regulates β-Cell Cholecystokinin Expression by Direct and Indirect Mechanisms (Abstract ID: 190968)

Liam D. Hurley, Rodsy Modhurima, Dawn Belt Davis, Michelle E. Kimple

us (code pays fourni par la source)

Gαz-independent and -dependent Improvements With EPA Supplementation on the Early Type 1 Diabetes Phenotype of NOD Mice

Rachel J. Fenske, Haley Wienkes, Darby C. Peter, Michael D. Schaid et autres

Abstract Prostaglandin E2 (PGE2) is a key mediator of inflammation and is derived from the omega-6 polyunsaturated fatty acid, arachidonic acid (AA). In the β-cell, the PGE2 receptor, Prostaglandin EP3 receptor (EP3), is coupled to the unique heterotrimeric G protein alpha subunit, …

us (code pays fourni par la source)

The prostaglandin E2 EP3 receptor has disparate effects on islet insulin secretion and content in β-cells in a high-fat diet-induced mouse model of obesity

Joshua C. Neuman, Austin Reuter, Kathryn A. Carbajal, Michael D. Schaid et autres

The EP3 receptor is a strong inhibitor of β-cell function and replication, suggesting it as a potential therapeutic target for the disease. Yet, EP3 has protective roles in extrapancreatic tissues. To address this, we designed β-cell-specific EP3 knockout mice and subjected them …

us (code pays fourni par la source)

Small molecule branched-chain ketoacid dehydrogenase kinase (BDK) inhibitors with opposing effects on BDK protein levels

Rachel J. Roth Flach, Eliza Bollinger, Allan R. Reyes, Brigitte Laforest et autres

Branched chain amino acid (BCAA) catabolic impairments have been implicated in several diseases. Branched chain ketoacid dehydrogenase (BCKDH) controls the rate limiting step in BCAA degradation, the activity of which is inhibited by BCKDH kinase (BDK)-mediated phosphorylation. Screening efforts to discover BDK …

us (code pays fourni par la source)

Ormdl3 regulation of specific ceramides is dispensable for mouse β-cell function and glucose homeostasis under obesogenic conditions

Liam D. Hurley, Hugo Lee, Gina Wade, Judith Simcox et autres

Chronic elevation of sphingolipids contributes to β-cell failure. ORMDL3 has been identified as a key regulator of sphingolipid homeostasis, however, its function in pancreatic β-cell pathophysiology remains unclear. Here, we generated a mouse model lacking Ormdl3 within pancreatic β-cells (Ormdl3β-/-). We show …

us (code pays fourni par la source)

Ormdl3 regulation of specific ceramides is dispensable for β-cell function and glucose homeostasis under obesogenic conditions

Liam D. Hurley, Hugo Lee, Gina Wade, Judith Simcox et autres

Abstract Chronic elevation of sphingolipids contributes to β-cell failure. ORMDL3 has been identified as a key regulator of sphingolipid homeostasis, however, its function in pancreatic β-cell pathophysiology remains unclear. Here, we generated a mouse model lacking Ormdl3 within pancreatic β-cells ( Ormdl3 …

us (code pays fourni par la source)

The Anticancer Agent Elesclomol Has Direct Effects on Mitochondrial Bioenergetic Function in Isolated Mammalian Mitochondria

Josephine S. Modica-Napolitano, Leena P. Bharath, Alison Hanlon, Liam D. Hurley

-thiobenzoylhydrazide)); formerly STA-4783) is a mitochondria-targeted chemotherapeutic agent that has demonstrated efficacy in selective cancer cell killing in pre-clinical and clinical testing. The biologically active form of elesclomol is a deprotonated copper chelate (elesclomol:copper; E:C), which has been shown to enhance reactive …

us (code pays fourni par la source)