Tim Bergner

Cryptosporidium sequentially remodels its single rhoptry into the host interface

Allison L. Cohen, Tim Bergner, Amandine Guérin, Maryse Lebrun et autres

Abstract The apicomplexan parasite Cryptosporidium is a leading cause of diarrheal disease in young children. Within the intestinal epithelium, Cryptosporidium establishes a unique intracellular niche in the apical brush border of enterocytes. A structurally complex interface between host and parasite acts as …

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A short linear motif, conserved from yeast to human, binds to members of the Spa2 family of cortical scaffold proteins

Lara Bareis, Aaron Siewert, Benjamin Grupp, Tim Bergner et autres

Tip growth is closely tied to fungal pathogenicity. Budding yeast Spa2 (the homolog of GIT1 and GIT2 in mammals), a multi-domain protein and member of the polarisome, orchestrates tip growth in yeasts and other fungi. We identified a conserved short linear motif …

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Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Johannes Georg Wieland, Jean Baptiste Roche, Tim Bergner, Rebecca Habisch et autres

Electron microscopy (EM) and especially three-dimensional (3D) EM techniques have become established methods in structural virology. Investigation of virus-induced alteration of the cellular ultrastructure, such as Zika virus (ZIKV)-induced replication factories or coronavirus replication organelles, demands 3D imaging. Transmission electron microscopy (TEM) …

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Weakly Supervised Virus Capsid Detection with Image-Level Annotations in Electron Microscopy Images

Hannah Kniesel, Leon Sick, Tristan Payer, Tim Bergner et autres

Current state-of-the-art methods for object detection rely on annotated bounding boxes of large data sets for training. However, obtaining such annotations is expensive and can require up to hundreds of hours of manual labor. This poses a challenge, especially since such annotations …

DeepEM Playground: Bringing deep learning to electron microscopy labs

Hannah Kniesel, Poonam Poonam, Tristan Payer, Tim Bergner et autres

Deep learning (DL) has transformed image analysis, enabling breakthroughs in segmentation, object detection, and classification. However, a gap persists between cutting-edge DL research and its practical adoption in electron microscopy (EM) labs. This is largely due to the inaccessibility of DL methods …

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A short linear motif, conserved from yeast to human, binds to members of the Spa2/GIT1 family of cortical scaffold proteins

Lara Bareis, Aaron Siewert, Tim Bergner, Clarissa Read et autres

Abstract Tip growth is closely tied to fungal pathogenicity. Spa2, a multi-domain protein and member of the polarisome, orchestrates tip growth in yeast and other fungi. We identified a conserved short linear motif in the RabGAPs Msb3 and Msb4, and the MAP …

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Distinct pathogenic mutations in ARF1 allow dissection of its dual role in cGAS-STING signalling

Johannes Lang, Tim Bergner, Julia Zinngrebe, Alice Lepelley et autres

Tight control of cGAS-STING-mediated DNA sensing is crucial to avoid auto-inflammation. The GTPase ADP-ribosylation factor 1 (ARF1) is crucial to maintain cGAS-STING homeostasis and various pathogenic ARF1 variants are associated with type I interferonopathies. Functional ARF1 inhibits STING activity by maintaining mitochondrial …

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Secondary Envelopment of Human Cytomegalovirus Is a Fast Process Utilizing the Endocytic Compartment as a Major Membrane Source

Tim Bergner, Laura Cortez Rayas, Gesa Freimann, Clarissa Read et autres

Secondary envelopment of the human cytomegalovirus (HCMV) is a critical but not well-understood process that takes place at the cytoplasmic viral assembly complex (cVAC) where nucleocapsids acquire their envelope by budding into cellular membranes containing viral glycoproteins. Previous studies presented controversial results …

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Secondary Envelopment of Human Cytomegalovirus Is a Fast Process Utilizing the Endocytic Compartment as a Major Membrane Source

Tim Bergner, Laura Cortez Rayas, Gesa Freimann, Clarissa Read et autres

Secondary envelopment of the human cytomegalovirus (HCMV) is a critical but not well-understood process that takes place at the cytoplasmic viral assembly complex (cVAC) where nucleocapsids acquire their envelope by budding into cellular membranes containing viral glycoproteins. Previous studies presented controversial results …

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In-vivo DAB cytochemistry and high-pressure freezing to determine the source of the human cytomegalovirus envelope

Tim Bergner, Laura Cortez Rayas, Jens von Einem, Clarissa Read

translocation to the cVAC and completion of the secondary envelopment process.Reducing the pulse-chase time to 30 minutes still resulted in WGA-labelled membranes that were used for secondary envelopment, suggesting that this process is even faster.STEM tomography further unambiguously identified capsid budding at …

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Secondary envelopment of the human cytomegalovirus is a fast process, utilizing the endocytic compartment

Tim Bergner, Laura Cortez Rayas, Jens von Einem, Clarissa Villinger

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ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling

Maximilian Hirschenberger, Alice Lepelley, Ulrich Rupp, Susanne Klute et autres

Type I interferon (IFN) signalling is tightly controlled. Upon recognition of DNA by cyclic GMP-AMP synthase (cGAS), stimulator of interferon genes (STING) translocates along the endoplasmic reticulum (ER)-Golgi axis to induce IFN signalling. Termination is achieved through autophagic degradation or recycling of …

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