Matthias Haury

PP7 and MS2 Virus-Like Particles as Complementary Standards for Cryo-EM

Mykhailo Kopylov, Mallak Ali, Joshua Hutchings, Jonathan Schwartz et autres

Reliable biological standards are required to validate cryo-electron microscopy (cryo-EM) workflows across single-particle analysis (SPA), cryo-electron tomography (cryo-ET), and in situ imaging. Mouse apoferritin (ApoF) remains the dominant high-resolution SPA reference. However, its utility is largely limited to high-resolution (≤4 Å) and …

us (code pays fourni par la source)

Characterization Standard for In-situ Cryo-electron Tomography

Mallak Ali, Joshua Hutchings, Tahiti Dutta, Nikki Jean et autres

Abstract Standardized biological specimens are essential for optimizing cryoEM workflows and benchmarking instrument performance. While apoferritin fulfills this role for single-particle analysis, no equivalent exists for cryo-electron tomography. Ribosomes are frequently used but require large datasets due to C1 symmetry and structural …

us (code pays fourni par la source)

A realistic phantom dataset for benchmarking cryo-ET data annotation

Ariana Peck, Yue Yu, Jonathan Schwartz, Anchi Cheng et autres

Cryo-electron tomography (cryo-ET) is a powerful technique for imaging molecular complexes in their native cellular environments. However, identifying the vast majority of molecular species in cellular tomograms remains prohibitively difficult. Machine learning (ML) methods provide an opportunity to automate the annotation process, …

us (code pays fourni par la source)

How to build the virtual cell with artificial intelligence: Priorities and opportunities

Charlotte Bunne, Yusuf Roohani, Yanay Rosen, Ankit Gupta et autres

Cells are essential to understanding health and disease, yet traditional models fall short of modeling and simulating their function and behavior. Advances in AI and omics offer groundbreaking opportunities to create an AI virtual cell (AIVC), a multi-scale, multi-modal large-neural-network-based model that …

us, ch, se, fr, de, es, ca, ae (code pays fourni par la source)

Annotating CryoET Volumes: A Machine Learning Challenge

Ariana Peck, Yue Yu, Jonathan Schwartz, Anchi Cheng et autres

Abstract Cryo-electron tomography (cryoET) has emerged as a powerful structural biology tool for understanding protein complexes in their native cellular environments. Presently, 3D volumes of cellular environments can be acquired in the thousands in a few days where each volume provides a …

us (code pays fourni par la source)

Signaling via the MyD88 Adaptor Protein in B Cells Suppresses Protective Immunity during Salmonella typhimurium Infection

Patrícia Neves, Vicky Lampropoulou, Elisabeth Calderón‐Gómez, Toralf Roch et autres

de, pt, ru, us (code pays fourni par la source)

Introducing Modern Science into Schools

J Willingale-Theune, Alexandra Manaia, Philipp Gebhardt, Rossana De Lorenzi et autres

A multinational research institution provides advanced training to help high-school teachers bring inquiry into the classroom.

de (code pays fourni par la source)

Impact of Retroviral Vector Components Stoichiometry on Packaging Cell Lines: Effects on Productivity and Vector Quality

Manuel J.T. Carrondo, Otto‐Wilhelm Merten, Matthias Haury, Paula M. Alves et autres

The productivity of retroviral vector producer cell lines is currently low; this can be attributed partly to disruption of the viral components stoichiometry originated by the dissociation of gag-pol, env, and viral genome. This paper addresses the impact of viral components stoichiometry …

pt, fr, de (code pays fourni par la source)

Stochastic Monoallelic Expression of IL-10 in T Cells

Dinis Pedro Calado, Tiago Paixão, Dan Holmberg, Matthias Haury

IL-10 is a potent anti-inflammatory and immunomodulatory cytokine, exerting major effects in the degree and quality of the immune response. Using a newly generated IL-10 reporter mouse model, which easily allows the study of IL-10 expression from each allele in a single …

pt, se (code pays fourni par la source)

The importance of 293 cell cycle phase on adenovirus vector production

Changhe Zhang, Tiago Borges Ferreira, Pedro E. Cruz, Paula M. Alves et autres

pt (code pays fourni par la source)

Transcriptional Profiling of Arabidopsis Tissues Reveals the Unique Characteristics of the Pollen Transcriptome

Jörg D. Becker, Leonor C. Boavida, Jorge Carneiro, Matthias Haury et autres

Pollen tubes are a good model for the study of cell growth and morphogenesis because of their extreme elongation without cell division. Yet, knowledge about the genetic basis of pollen germination and tube growth is still lagging behind advances in pollen physiology …

pt (code pays fourni par la source)

Selective Gene Expression of Latent Murine Gammaherpesvirus 68 in B Lymphocytes

Sofia Marques, Stacey Efstathiou, Kenneth G. C. Smith, Matthias Haury et autres

Intranasal infection of mice with murine gammaherpesvirus 68 (MHV-68), a virus genetically related to the human pathogen Kaposi's sarcoma-associated herpesvirus, results in a persistent, latent infection in the spleen and other lymphoid organs. Here, we have determined the frequency of virus infection …

pt, gb (code pays fourni par la source)