Dazhang Bai

Single-cell multiomics data analysis of potential receptors and therapeutic drugs for epilepsy patients comorbid with depression

Guiqin Bai, Xuerong Zhou, Cheng Xiong, Xi Kang et autres

Depression frequently cooccurs with epilepsy (EP) and has become a focus of clinical management, but effective pharmacological interventions remain limited. In this study, single-cell RNA sequencing (scRNA-seq) data were analyzed to identify changes in oligodendrocyte precursor cells (OPCs) in EP and major …

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Alantolactone Inhibits Double Expression Lymphoma via Dual-Targeted Glycogen Synthase Kinase 3 Beta and B-Cell Lymphoma2

Jianhua Chen, Qing Liao, Sha Yang, Jiaojiao Bian et autres

Background: Dual-expression lymphoma (DEL) is an aggressive subtype with concurrent MYC and BCL2 overexpression. This disease exhibits a poor prognosis and responds poorly to standard R-CHOP therapy,highlighting the urgent need for novel treatments. Alantolactone (ALA), a natural compound, has shown anticancer potential, …

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Tungsten-based polyoxometalate nanoclusters as ferroptosis inhibitors modulating S100A8/A9-mediated iron metabolism pathway for managing intracerebral haemorrhage

Yang Yang, Mingzhu Lv, Ruihong Liu, Paul B. Yu et autres

BACKGROUND: Intracerebral haemorrhage (ICH) is a devastating neurological disorder with high morbidity and mortality rates, largely owing to the lack of effective therapeutic strategies. Growing evidence has underscored the pivotal role of ferroptosis in intracerebral haemorrhage, and its contribution to neuronal death …

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Optimizing total RNA extraction method for human and mice samples

Yumei Zeng, Xiaoxue Tang, Jinwen Chen, Xi Kang et autres

Background Extracting high-quality total RNA is pivotal for advanced RNA molecular studies, such as Next-generation sequencing and expression microarrays where RNA is hybridized. Despite the development of numerous extraction methods in recent decades, like the cetyl-trimethyl ammonium bromide (CTAB) and the traditional …

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Low-dose diazepam improves cognitive function in APP/PS1 mouse models: Involvement of AMPA receptors

Junwen Chen, Ming Zhang, Ziyi Shen, Ming Tang et autres

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Pathogenic TDP ‐43 accelerates the generation of toxic exon1 HTT in Huntington's disease knock‐in mice

Dazhang Bai, Fuyu Deng, Qingqing Jia, Kaili Ou et autres

Huntington's disease (HD) is caused by a CAG repeat expansion in exon1 of the HTT gene that encodes a polyglutamine tract in huntingtin protein. The formation of HTT exon1 fragments with an expanded polyglutamine repeat has been implicated as a key step …

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Pathologic TDP ‐43 downregulates myelin gene expression in the monkey brain

Longhong Zhu, Dazhang Bai, Xiang Wang, Kaili Ou et autres

Growing evidence indicates that non-neuronal oligodendrocyte plays an important role in Amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases. In patient's brain, the impaired myelin structure is a pathological feature with the observation of TDP-43 in cytoplasm of oligodendrocyte. However, the mechanism …

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Loss of TDP-43 mediates severe neurotoxicity by suppressing PJA1 gene transcription in the monkey brain

Longhong Zhu, Fuyu Deng, Dazhang Bai, Junqi Hou et autres

The nuclear loss and cytoplasmic accumulation of TDP-43 (TAR DNA/RNA binding protein 43) are pathological hallmarks of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Previously, we reported that the primate-specific cleavage of TDP-43 accounts for its cytoplasmic mislocalization in patients' …

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Comparing HD knockin pigs and mice reveals the pathological role of IL-17

Qingqing Jia, Dazhang Bai, Xiao Zheng, Longhong Zhu et autres

Our previous work has established a knockin (KI) pig model of Huntington's disease (HD) that can replicate the typical pathological features of HD, including selective striatal neuronal loss, reactive gliosis, and axonal degeneration. However, HD KI mice exhibit milder neuropathological phenotypes and …

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Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington’s disease knock-in mice

Dazhang Bai, Longhong Zhu, Qingqing Jia, Xuezhi Duan et autres

TAR binding protein 43 (TDP-43) is normally present in the nucleus but mislocalized in the cytoplasm in a number of neurodegenerative diseases including Huntington's disease (HD). The nuclear loss of TDP-43 impairs gene transcription and regulation. However, it remains to be investigated …

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Ketogenic diet alleviates brain iron deposition and cognitive dysfunction via Nrf2-mediated ferroptosis pathway in APP/PS1 mouse

Yaya Qin, Dazhang Bai, Ming Tang, Ming Zhang et autres

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Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington’s disease knock-in mice

Peng Yin, Xiao‐Jiang Li, Dazhang Bai

RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.