John E. Dueber

Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield

Lauren Narcross, Michael E. Pyne, Kaspar Kevvai, Ka‐Hei Siu et autres

High Resolution Image Download MS PowerPoint Slide The benzylisoquinoline alkaloid (BIA) family of tetrahydroisoquinolines (THIQs) comprises >2,500 members, including the pharmaceuticals morphine, codeine, and papaverine, as well as the antibiotics sanguinarine and chelerythrine. Agricultural cultivation can supply the demand for the BIAs …

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Functional targeting of membrane transporters and enzymes to peroxisomes

Ka‐Hei Siu, John E. Dueber

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Nickase fidelity drives EvolvR-mediated diversification in mammalian cells

Juan E. Hurtado, Adam J. Schieferecke, Shakked O. Halperin, Dylan Aidlen et autres

In vivo genetic diversifiers have previously enabled efficient searches of genetic variant fitness landscapes for continuous directed evolution. However, existing genomic diversification modalities for mammalian genomic loci exclusively rely on deaminases to generate transition mutations within target loci, forfeiting access to most …

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Author Correction: ML-enhanced peroxisome capacity enables compartmentalization of multienzyme pathway

Jordan J. Baker, Jie Shi, Shangying Wang, Elena M. Mujica et autres

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ML-enhanced peroxisome capacity enables compartmentalization of multienzyme pathway

Jordan J. Baker, Jie Shi, Shangying Wang, Elena M. Mujica et autres

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Ten Years of the Synthetic Biology Summer Course at Cold Spring Harbor Laboratory

Karmella Ann Haynes, Lauren B. Andrews, Chase L. Beisel, James Chappell et autres

The Cold Spring Harbor Laboratory (CSHL) Summer Course on Synthetic Biology, established in 2013, has emerged as a premier platform for immersive education and research in this dynamic field. Rooted in CSHL's rich legacy of biological discovery, the course offers a comprehensive …

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Examining hydrogen peroxide-containing organelles in seaweeds

Joseph S. Ramahi, Keiko L Hokeness, John A. Gonzales, Raúl A. Márquez et autres

. Our study introduces an effective survey tool to identify promising seaweed species abundant in bromoform from diverse marine habitats.

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Engineering an Escherichia coli strain for production of long single-stranded DNA

Konlin Shen, Jake J. Flood, Zhihuizi Zhang, Alvin S. Ha et autres

Long single-stranded DNA (ssDNA) is a versatile molecular reagent with applications including RNA-guided genome engineering and DNA nanotechnology, yet its production is typically resource-intensive. We introduce a novel method utilizing an engineered Escherichia coli 'helper' strain and phagemid system that simplifies long …

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Engineering an Escherichia coli strain for production of long single-stranded DNA

Konlin Shen, Jake J. Flood, Zhihuizi Zhang, Alvin S. Ha et autres

Abstract Long single-stranded DNA (ssDNA) is a versatile molecular reagent with applications including RNA- guided genome engineering and DNA nanotechnology, yet its production is typically resource-intensive. We introduce a novel method utilizing an engineered E. coli “helper” strain and phagemid system that …

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Benzylisoquinoline alkaloid production in yeast via norlaudanosoline improves selectivity and yield

Lauren Narcross, Michael E. Pyne, Kaspar Kevvai, Ka‐Hei Siu et autres

Abstract The benzylisoquinoline alkaloid (BIA) family of tetrahydroisoquinolines (THIQs) comprises over 2,500 members, including the pharmaceuticals morphine, codeine, and papaverine as well as the antibiotics sanguinarine and chelerythrine used in animal husbandry. Agricultural cultivation can currently supply the demand for the BIAs …

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Abstract 2448: Evaluation of peroxisome size regulation through optogenetic enlargement for future use as synthetic organelle in Saccharomyces cerevisiae

Nelson Menjivar, Mark Y. Chan, Jordan Baker, John E. Dueber

In vivo hypermutation and continuous evolution

Rosana S. Molina, Gordon Rix, Amanuella A. Mengiste, Beatriz Álvarez et autres

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