Accès ouvert
2026
article
OpenAlex
Nathaniel Howitz, Stephen N. Rondthaler, Lauren B. Andrews
Orthogonal repressor-based logic gates have enabled the predictive design of genetic circuits using automated design algorithms. However, engineering complex genetic circuits has been limited by the availability of transcriptional logic gates that are orthogonal, exhibit low cytotoxicity, and have a large dynamic …
us
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2026
article
OpenAlex
Chinmay P. Deshpande, Shradha Khanduja, Stephen N. Rondthaler, Lauren B. Andrews et autres
Many bacterial immunotherapies, because of their intracellular lifestyle, have the potential to overcome the limitations that reduce the efficacy of immune checkpoint inhibitors. One reason this approach is not completely curative is the innate immune suppression of the bacteria. After cell invasion, …
us, in, ru
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Accès ouvert
2026
article
OpenAlex
Hyerim Ban, Stephen N. Rondthaler, Matthew Lebovich, Marcos A. Lora et autres
strains, providing guidelines for diverse applications of these tools.
us, in
(code pays fourni par la source)
Accès ouvert
2026
preprint
OpenAlex
Hyerim Ban, Stephen N. Rondthaler, Matthew Lebovich, Marcos A. Lora et autres
Abstract CRISPR interference (CRISPRi) has emerged as a versatile approach for targeted gene repression in many organisms, including microbes and bacteria, due to the simple design of sequence-specific transcriptional silencing of gene expression. However, the strain-specific effects on repression efficiency and the …
us
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Accès ouvert
2024
editorial
OpenAlex
Karmella Ann Haynes, Lauren B. Andrews, Chase L. Beisel, James Chappell et autres
The Cold Spring Harbor Laboratory (CSHL) Summer Course on Synthetic Biology, established in 2013, has emerged as a premier platform for immersive education and research in this dynamic field. Rooted in CSHL's rich legacy of biological discovery, the course offers a comprehensive …
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2024
article
OpenAlex
Min Zeng, Biprodev Sarker, Stephen N. Rondthaler, Vanessa T. Vu et autres
Programmable intercellular signaling using components of naturally occurring quorum sensing can allow for coordinated functions to be engineered in microbial consortia. LuxR-type transcriptional regulators are widely used for this purpose and are activated by homoserine lactone (HSL) signals. However, they often suffer …
us
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Accès ouvert
2024
article
OpenAlex
Matthew Lebovich, Jared Gracia-David, Lauren B. Andrews
Nissle 1917 (EcN) offer a strategy to sense and modulate the concentration of metabolites or therapeutics in the gastrointestinal tract. Here, we present an approach to regulate the production of the depression-associated metabolite gamma-aminobutyric acid (GABA) in EcN using genetic circuits that …
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Accès ouvert
2023
article
OpenAlex
Min Zeng, Biprodev Sarker, Nathaniel Howitz, Ishita Shah et autres
A universal biochemical signal for bacterial cell–cell communication could facilitate programming dynamic responses in diverse bacterial consortia. However, the classical quorum sensing paradigm is that Gram-negative and Gram-positive bacteria generally communicate via homoserine lactones (HSLs) or oligopeptide molecular signals, respectively, to elicit …
us
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Accès ouvert
2023
article
OpenAlex
Stephen N. Rondthaler, Biprodev Sarker, Nathaniel Howitz, Ishita Shah et autres
Staphylococcus aureus is an important clinical bacterium prevalent in human-associated microbiomes and the cause of many diseases. However, S. aureus has been intractable to synthetic biology approaches due to limited characterized genetic parts for this nonmodel Gram-positive bacterium. Moreover, genetic manipulation of …
us
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Accès ouvert
2023
preprint
OpenAlex
Min Zeng, Biprodev Sarker, Stephen N. Rondthaler, Vanessa T. Vu et autres
ABSTRACT Programmable intercellular signaling using components of naturally-occurring quorum sensing can allow for coordinated functions to be engineered in microbial consortia. LuxR-type transcriptional regulators are widely used for this purpose and are activated by homoserine lactone (HSL) signals. However, they often suffer …
us
(code pays fourni par la source)
Accès ouvert
2023
preprint
OpenAlex
Min Zeng, Biprodev Sarker, Nathaniel Howitz, Ishita Shah et autres
Abstract A universal biochemical signal for bacterial cell-cell communication could facilitate programming dynamic responses in diverse bacterial consortia. However, the classical quorum sensing paradigm is that gram-negative and gram-positive bacteria generally communicate via homoserine lactones (HSL) or oligopeptide molecular signals, respectively, to …
us
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Matthew Lebovich, Min Zeng, Lauren B. Andrews
Through the implementation of designable genetic circuits, engineered probiotic microorganisms could be used as noninvasive diagnostic tools for the gastrointestinal tract. For these living cells to report detected biomarkers or signals after exiting the gut, the genetic circuits must be able to …
us
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