Oliver Meikar

Engineered Bacterial Ligand From Streptococcus pneumoniae on Macroporous Resins for Selective Affinity Capture of Secretory IgA

David Scheich, Andres Männik, Oliver Meikar, Joan Teyra et autres

Secretory immunoglobulin A (sIgA) is a promising emerging biopharmaceutical candidate, but it currently lacks a standardized platform for purification. To address this, a novel affinity chromatography resin was developed by immobilizing protein variants of the Streptococcus pneumoniae surface protein, SpsA, as an …

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STAGETOOL, a Novel Automated Approach for Mouse Testis Histological Analysis

Oliver Meikar, Daniel Majoral, Olli Heikkinen, Eero Valkama et autres

Spermatogenesis is a complex differentiation process that takes place in the seminiferous tubules. A specific organization of spermatogenic cells within the seminiferous epithelium enables a synchronous progress of germ cells at certain steps of differentiation on the spermatogenic pathway. This can be …

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Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Matteo Da Ros, Tiina Lehtiniemi, Opeyemi Olotu, Oliver Meikar et autres

To characterize each step of spermatogenesis, researchers must separate different subpopulations of germ cells from testes. However, isolating discrete populations is challenging, because the adult testis contains a complex mix of germ cells from all steps of spermatogenesis along with certain populations …

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Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Matteo Da Ros, Tiina Lehtiniemi, Opeyemi Olotu, Oliver Meikar et autres

To characterize each step of spermatogenesis, researchers must separate different subpopulations of germ cells from testes. However, isolating discrete populations is challenging, because the adult testis contains a complex mix of germ cells from all steps of spermatogenesis along with certain populations …

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piRNA-directed cleavage of meiotic transcripts regulates spermatogenesis

W.S. Sho Goh, Ilaria Falciatori, Oliver H. Tam, Ralph Burgess et autres

MIWI catalytic activity is required for spermatogenesis, indicating that piRNA-guided cleavage is critical for germ cell development. To identify meiotic piRNA targets, we augmented the mouse piRNA repertoire by introducing a human meiotic piRNA cluster. This triggered a spermatogenesis defect by inappropriately …

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An atlas of chromatoid body components

Oliver Meikar, Vasily V. Vagin, Frédéric Chalmel, Karin Sõstar et autres

The genome of male germ cells is actively transcribed during spermatogenesis to produce phase-specific protein-coding mRNAs and a considerable amount of different noncoding RNAs. Ribonucleoprotein (RNP) granule-mediated RNA regulation provides a powerful means to secure the quality and correct expression of the …

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Isolation of Chromatoid Bodies from Mouse Testis as a Rich Source of Short RNAs

Oliver Meikar, Noora Kotaja

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Chromatoid body mediated RNA regulation in mouse male germline

Oliver Meikar

Male germ cell differentiation, spermatogenesis is an exceptional developmental process that produces a massive amount of genetically unique spermatozoa. The complexity of this process along with the technical limitations in the germline research has left many aspects of spermatogenesis poorly understood. Post-meiotic …

Epigenetic Regulation of Male Germ Cell Differentiation

Oliver Meikar, Matteo Da Ros, Noora Kotaja

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The RNA Binding Protein SAM68 Transiently Localizes in the Chromatoid Body of Male Germ Cells and Influences Expression of Select MicroRNAs

Valeria Messina, Oliver Meikar, Maria Paola Paronetto, Sara Calabretta et autres

The chromatoid body (CB) is a unique structure of male germ cells composed of thin filaments that condense into a perinuclear organelle after meiosis. Due to the presence of proteins involved in different steps of RNA metabolism and of different classes of …

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Dicer1 Depletion in Male Germ Cells Leads to Infertility Due to Cumulative Meiotic and Spermiogenic Defects

Yannick Romero, Oliver Meikar, Marilena D. Papaioannou, Béatrice Conne et autres

BACKGROUND: Spermatogenesis is a complex biological process that requires a highly specialized control of gene expression. In the past decade, small non-coding RNAs have emerged as critical regulators of gene expression both at the transcriptional and post-transcriptional level. DICER1, an RNAse III …

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Dicer Is Required for Haploid Male Germ Cell Differentiation in Mice

Hanna Korhonen, Oliver Meikar, Ram Prakash Yadav, Marilena D. Papaioannou et autres

BACKGROUND: The RNase III endonuclease Dicer is an important regulator of gene expression that processes microRNAs (miRNAs) and small interfering RNAs (siRNAs). The best-characterized function of miRNAs is gene repression at the post-transcriptional level through the pairing with mRNAs of protein-encoding genes. …

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