The RNA Binding Protein SAM68 Transiently Localizes in the Chromatoid Body of Male Germ Cells and Influences Expression of Select MicroRNAs
Rattachement africain : it, fi. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The chromatoid body (CB) is a unique structure of male germ cells composed of thin filaments that condense into a perinuclear organelle after meiosis. Due to the presence of proteins involved in different steps of RNA metabolism and of different classes of RNAs, including microRNAs (miRNAs), the CB has been recently suggested to function as an RNA processing centre. Herein, we show that the RNA binding protein SAM68 transiently localizes in the CB, in concomitance with the meiotic divisions of mouse spermatocytes. Precise staging of the seminiferous tubules and co-localization studies with MVH and MILI, two well recognized CB markers, documented that SAM68 transiently associates with the CB in secondary spermatocytes and early round spermatids. Furthermore, although SAM68 co-immunoprecipitated with MVH in secondary spermatocytes, its ablation did not affect the proper localization of MVH in the CB. On the other hand, ablation of the CB constitutive component MIWI did not impair association of SAM68 with the CB. Isolation of CBs from Sam68 wild type and knockout mouse testes and comparison of their protein content by mass spectrometry indicated that Sam68 ablation did not cause overall alterations in the CB proteome. Lastly, we found that SAM68 interacts with DROSHA and DICER in secondary spermatocytes and early round spermatids and that a subset of miRNAs were altered in Sam68(-/-) germ cells. These results suggest a novel role for SAM68 in the miRNA pathway during spermatogenesis.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The RNA Binding Protein SAM68 Transiently Localizes in the Chromatoid Body of Male Germ Cells and Influences Expression of Select MicroRNAs
- Date Crossref
- 22/06/2012
- Éditeur
- Public Library of Science (PLoS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Rome Tor Vergata Department of Public Health and Cell Biology pays non établi dans la noticeUniversité ou école supérieure
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Fondazione Santa Lucia pays non établi dans la noticeÉtablissement de santé
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University of Turku Department of Physiology pays non établi dans la noticeUniversité ou école supérieure
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Foro Italico University of Rome pays non établi dans la noticeUniversité ou école supérieure
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Sapienza University of Rome pays non établi dans la noticeUniversité ou école supérieure
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Laboratory of Neuroembryology pays non établi dans la noticeStructure de recherche
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University of Rome "Tor Vergata" Department of Public Health and Cell Biology pays non établi dans la noticeUniversité ou école supérieure
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University of Rome “Foro Italico” Department of Health Sciences pays non établi dans la noticeUniversité ou école supérieure
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University of Rome "Foro Italico" Department of Health Sciences pays non établi dans la noticeUniversité ou école supérieure
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University of Rome “La Sapienza” II Medical School pays non établi dans la noticeUniversité ou école supérieure
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University of Rome “Tor Vergata” Department of Public Health and Cell Biology pays non établi dans la noticeUniversité ou école supérieure
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University of Rome "La Sapienza" II Medical School pays non établi dans la noticeUniversité ou école supérieure
Department of Public Health and Cell Biology — University of Rome Tor Vergata, Fondazione Santa Lucia et Department of Physiology — University of Turku, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.