Zhaobo Lang

Efficient genome editing in tomato using Cas12i3 variants

Siqun Wu, Lei Zhu, Meili Zhou, Bo Yu et autres

Systematic comparison of Cas12i gene-editing tools in tomato identifies Cas-SF01 as the most efficient variant, achieving editing efficiencies comparable to conventional Cas9 at some genomic sites and providing a compact, highly specific, and versatile tool for studying gene function, engineering fruit metabolism, …

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DNA hypomethylation enables the transcriptional repressor SlSPL-CNR to control fruit flavor ester biosynthesis

Zhifeng Zeng, Yu Ma, Hang He, Li Li et autres

Reduction of DNA methylation has traditionally been associated with gene activation. Here, we show that DNA hypomethylation permits the binding of a transcriptional repressor, leading to gene silencing. In tomato, the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE TF SlSPL-CNR exhibits methylation-sensitive DNA binding and …

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Tandem MADS ‐Box Genes FUL2 and MADS1 Form a Regulatory Module to Repress Serotonin Biosynthesis via Direct ASMT5 Activation in Tomato Fruit

Yaping Xu, Huimin Jia, Dengguo Tang, Zhang Li et autres

The regulation of serotonin metabolism during fruit development and ripening remains poorly understood, despite its potential roles in plant defence and human nutrition. Here, we demonstrated that the MADS-box transcription factor FUL2 acts as a key repressor of serotonin accumulation in tomato …

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DML2-Mediated Activation of RIN Represses Pectin Methyltransferase Genes to Modulate Fruit Firmness during Tomato Ripening

Yunong Li, Tian Li, Zhifeng Zeng, Qingyuan Wang et autres

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Genomic insights into stepwise selection reshaping fruit traits and male-biased selection driving hermaphroditism in papayas

Changmian Ji, Ruizong Jia, Yuliang Zhang, Xiaoxi Du et autres

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Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis

Li He, Yao Yao, Xiaofei Wei, Wei Yuan et autres

Tools to edit DNA methylation in a targeted manner are vital for establishing causal relationships between DNA methylation and its function, as well as for plant breeding and gene therapy. Here, by constructing dCas9 fusions to a panel of effectors and cofactors, …

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An efficient tissue-culture-free soybean genetic transformation technology using the extremely simple cut-dip-budding strategy

Xuesong Cao, Hongtao Xie, Zhonghui Wang, Fengxue Jing et autres

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A mutually antagonistic mechanism mediated by RNA m6A modification in plant-virus interactions

Jia-Hui Liu, Yujing Lin, Yingxin Li, Zhaobo Lang et autres

RNA-based immunity plays a central role in host defense against pathogens, with both hosts and pathogens continually evolving antagonistic strategies in their ongoing arms race. Although the presence of N6-methyl-adenosine (m6A) in viruses has been recognized for decades, its functional significance in …

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Maintaining basal B‐RAF kinase activity for abscisic acid signaling via reciprocal phosphoregulation of a single serine residue

Chen Zhu, Tian Sang, Zhen Zhang, Yubei Wang et autres

ABSTRACT The phytohormone abscisic acid (ABA) regulates plant responses to environmental stresses, development, and immunity. Under unfavorable conditions, ABA forms a complex with its receptor proteins Pyrabactin Resistance 1 (PYR1)/PYR1‐likes (PYLs)/Regulatory Component of ABA Receptors (RCARs), inhibiting Clade A Protein Phosphatases Type …

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Rice B2 and B3 subgroup RAF kinases play central roles in both ABA and osmotic stress signaling

Xinyong Wang, Hongliang Wang, Zhen Zhang, Yubei Wang et autres

Protein kinases in the mitogen-activated protein kinase kinase kinase (MAPKKK) superfamily are known to be central actors in orchestrating cell differentiation, growth, and responses to extracellular stimuli in eukaryotes. However, the rice (Oryza sativa L.) MAPKKKs, especially the rapidly accelerated fibrosarcoma (RAF) …

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Reduced content of gamma‐aminobutyric acid enhances resistance to bacterial wilt disease in tomato

Achen Zhao, Qian Li, Pengfei Meng, Ping Liu et autres

Bacteria within the Ralstonia solanacearum species complex cause devastating diseases in numerous crops, causing important losses in food production and industrial supply. Despite extensive efforts to enhance plant tolerance to disease caused by Ralstonia, efficient and sustainable approaches are still missing. Before, …

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Tomato MADS ‐ RIN regulates GAME5 expression to promote non‐bitter glycoalkaloid biosynthesis in fruit

Yinhuan Xie, Yaping Xu, Huimin Jia, Ke Wang et autres

A well-known defense-associated steroidal glycoalkaloid (SGA) metabolic shift eliminates the bitterness and toxicity of ripe tomato fruits. This study was conducted to clarify the effects of MADS-RIN (RIN) and its cofactors on SGA metabolism in tomato fruits. Using a CRISPR/Cas9-based gene-editing system, …

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