A mutually antagonistic mechanism mediated by RNA m6A modification in plant-virus interactions
Rattachement africain : cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
RNA-based immunity plays a central role in host defense against pathogens, with both hosts and pathogens continually evolving antagonistic strategies in their ongoing arms race. Although the presence of N6-methyl-adenosine (m6A) in viruses has been recognized for decades, its functional significance in plant antiviral defenses has only recently been revealed. Moreover, viral counterstrategies targeting m6A-mediated defenses remain largely unexplored. Here, we uncover a mutually antagonistic mechanism between m6A-mediated antiviral defense and a countermeasure employed by the RNA virus Cucumber mosaic virus (CMV). The deposition of m6A modification on CMV genomic RNAs was validated through m6A antibody-mediated MeRIP and nanopore-based direct RNA sequencing (DRS). During infection, plant m6A methyltransferases are translocated to the cytoplasm through their interaction with the viral coat protein (CP), facilitating viral m6A deposition. The plant EVOLUTIONARILY CONSERVED C-TERMINAL REGION 8 (ECT8) protein acts as a reader of viral m6A, destabilizing viral RNAs and mediating antiviral activity. Conversely, the CMV-2b protein, known as a viral suppressor of RNA silencing (VSR), antagonizes this defense by inhibiting viral m6A deposition. This occurs via direct interactions between 2b and the m6A methyltransferase components MTB and HAKAI, disrupting the methyltransferase complex’s functionality. Furthermore, CMV-2b also downregulates global plant m6A levels, leading to the misexpression of defense-related transcripts. Collectively, our findings elucidate a previously unrecognized layer of host-virus interaction in which m6A modification serves as a regulatory battleground, positioning m6A dynamics as a new frontier in plant-virus coevolution. Liu et al. reveal that m6A RNA modification enhances plant antiviral defense against Cucumber mosaic virus, while the virus counters by suppressing m6A deposition, highlighting a molecular battleground in plant–virus interaction.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A mutually antagonistic mechanism mediated by RNA m6A modification in plant-virus interactions
- Date Crossref
- 24/11/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Chinese Academy of Sciences pays non établi dans la noticeOrganisme public
-
Center for Excellence in Molecular Plant Sciences pays non établi dans la noticeUniversité ou école supérieure
-
University of Chinese Academy of Sciences pays non établi dans la noticeUniversité ou école supérieure
-
Southern University of Science and Technology pays non établi dans la noticeUniversité ou école supérieure
-
South China Normal University pays non établi dans la noticeUniversité ou école supérieure
-
State Key Laboratory of Plant Trait Design pays non établi dans la noticeStructure de recherche
-
Institute of Advanced Biotechnology and School of Medicine pays non établi dans la noticeUniversité ou école supérieure
-
School of Life Science Guangdong Provincial Key Laboratory of Biotechnology for Plant Development pays non établi dans la noticeUniversité ou école supérieure
Chinese Academy of Sciences, Center for Excellence in Molecular Plant Sciences et University of Chinese Academy of Sciences, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.